EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2.
ジャーナル・号・ページ	PLoS Pathog, Vol. 14, Issue 8, Page e1007236, Year 2018
掲載日	2018年8月13日
著者	Wenfei Song / Miao Gui / Xinquan Wang / Ye Xiang /
PubMed 要旨	The trimeric SARS coronavirus (SARS-CoV) surface spike (S) glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion ...The trimeric SARS coronavirus (SARS-CoV) surface spike (S) glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to postfusion conformation transition. Here, we report the structure of the SARS-CoV S glycoprotein in complex with its host cell receptor ACE2 revealed by cryo-electron microscopy (cryo-EM). The complex structure shows that only one receptor-binding domain of the trimeric S glycoprotein binds ACE2 and adopts a protruding "up" conformation. In addition, we studied the structures of the SARS-CoV S glycoprotein and its complexes with ACE2 in different in vitro conditions, which may mimic different conformational states of the S glycoprotein during virus entry. Disassociation of the S1-ACE2 complex from some of the prefusion spikes was observed and characterized. We also characterized the rosette-like structures of the clustered SARS-CoV S2 trimers in the postfusion state observed on electron micrographs. Structural comparisons suggested that the SARS-CoV S glycoprotein retains a prefusion architecture after trypsin cleavage into the S1 and S2 subunits and acidic pH treatment. However, binding to the receptor opens up the receptor-binding domain of S1, which could promote the release of the S1-ACE2 complex and S1 monomers from the prefusion spike and trigger the pre- to postfusion conformational transition.
リンク	PLoS Pathog / PubMed:30102747 / PubMed Central
手法	EM (単粒子)
解像度	3.6 - 30.5 Å
構造データ	EMDB-9583: Trypsin-cleaved SARS-CoV spike glycoprotein and ACE2 complex, ACE2-free conformation with three RBD in down position 手法: EM (単粒子) / 解像度: 8.3 Å EMDB-9584: Trypsin-cleaved SARS-CoV spike glycoprotein and ACE2 complex, ACE2-free conformation with one RBD in up position 手法: EM (単粒子) / 解像度: 9.0 Å EMDB-9585: Trypsin-cleaved SARS-CoV spike glycoprotein and ACE2 complex, ACE2-bound conformation 1 手法: EM (単粒子) / 解像度: 19.7 Å EMDB-9586: Trypsin-cleaved SARS-CoV spike glycoprotein and ACE2 complex, ACE2-bound conformation 2 手法: EM (単粒子) / 解像度: 9.0 Å EMDB-9587: Trypsin-cleaved SARS-CoV spike glycoprotein and ACE2 complex, ACE2-bound conformation 3 手法: EM (単粒子) / 解像度: 18.5 Å 9588 6acc EMDB-9588, PDB-6acc: Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, ACE2-free conformation with three RBD in down conformation 手法: EM (単粒子) / 解像度: 3.6 Å 9589 6acd EMDB-9589, PDB-6acd: Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, ACE2-free conformation with one RBD in up conformation 手法: EM (単粒子) / 解像度: 3.9 Å 9591 6acg EMDB-9591, PDB-6acg: Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, ACE2-bound conformation 1 手法: EM (単粒子) / 解像度: 5.4 Å 9593 6acj EMDB-9593, PDB-6acj: Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, ACE2-bound conformation 2 手法: EM (単粒子) / 解像度: 4.2 Å 9594 6ack EMDB-9594, PDB-6ack: Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, ACE2-bound conformation 3 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-9595: Trypsin-cleaved SARS-CoV spike glycoprotein 手法: EM (単粒子) / 解像度: 6.8 Å EMDB-9596: Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein 手法: EM (単粒子) / 解像度: 6.7 Å EMDB-9597: Postfusion state of SARS-CoV spike glycoprotein 手法: EM (単粒子) / 解像度: 30.5 Å EMDB-9598: Trypsin-cleaved and low pH-treated SARS-CoV spike glycoprotein and ACE2 complex, dissociated S1-ACE2 手法: EM (単粒子) / 解像度: 11.6 Å
由来	SARS coronavirus (SARS コロナウイルス) homo sapiens (ヒト) human sars coronavirus (ウイルス)
キーワード	VIRAL PROTEIN / SARS-CoV / spike / glycoprotein / Class I fusion protein / membrane fusion / VIRAL PROTEIN/HYDROLASE / VIRAL PROTEIN-HYDROLASE complex