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Structure paper

TitleStructural and functional studies of LRP6 ectodomain reveal a platform for Wnt signaling.
Journal, issue, pagesDev Cell, Vol. 21, Issue 5, Page 848-861, Year 2011
Publish dateNov 15, 2011
AuthorsShuo Chen / Doryen Bubeck / Bryan T MacDonald / Wen-Xue Liang / Jian-Hua Mao / Tomas Malinauskas / Oscar Llorca / A Radu Aricescu / Christian Siebold / Xi He / E Yvonne Jones /
PubMed AbstractLDL-receptor-related protein 6 (LRP6), alongside Frizzled receptors, transduces Wnt signaling across the plasma membrane. The LRP6 ectodomain comprises four tandem β-propeller-EGF-like domain (PE) ...LDL-receptor-related protein 6 (LRP6), alongside Frizzled receptors, transduces Wnt signaling across the plasma membrane. The LRP6 ectodomain comprises four tandem β-propeller-EGF-like domain (PE) pairs that harbor binding sites for Wnt morphogens and their antagonists including Dickkopf 1 (Dkk1). To understand how these multiple interactions are integrated, we combined crystallographic analysis of the third and fourth PE pairs with electron microscopy (EM) to determine the complete ectodomain structure. An extensive inter-pair interface, conserved for the first-to-second and third-to-fourth PE interactions, contributes to a compact platform-like architecture, which is disrupted by mutations implicated in developmental diseases. EM reconstruction of the LRP6 platform bound to chaperone Mesd exemplifies a binding mode spanning PE pairs. Cellular and binding assays identify overlapping Wnt3a- and Dkk1-binding surfaces on the third PE pair, consistent with steric competition, but also suggest a model in which the platform structure supports an interplay of ligands through multiple interaction sites.
External linksDev Cell / PubMed:22000855 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.9 - 26.0 Å
Structure data

EMDB-1964:
Structural and Functional Studies of LRP6 Ectodomain Reveal a Platform for Wnt Signaling
Method: EM (single particle) / Resolution: 25.0 Å

EMDB-1965:
Structural and Functional Studies of LRP6 Ectodomain Reveal a Platform for Wnt Signaling
Method: EM (single particle) / Resolution: 26.0 Å

PDB-4a0p:
Crystal structure of LRP6P3E3P4E4
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-CA:
Unknown entry

ChemComp-CL:
Unknown entry / Chloride

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-HOH:
WATER / Water

Source
  • homo sapiens (human)
KeywordsSIGNALING / LRP6 / WNT SIGNALLING / WNT3A / DKK1 / MESD

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