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| Title | Evolutionary adaptations of doublet microtubules in trypanosomatid parasites. |
|---|---|
| Journal, issue, pages | Science, Vol. 387, Issue 6739, Page eadr5507, Year 2025 |
| Publish date | Mar 14, 2025 |
 Authors | Matthew H Doran / Qingwei Niu / Jianwei Zeng / Tom Beneke / James Smith / Peter Ren / Sophia Fochler / Adrian Coscia / Johanna L Höög / Shimi Meleppattu / Polina V Lishko / Richard J Wheeler / Eva Gluenz / Rui Zhang / Alan Brown /     |
| PubMed Abstract | The movement and pathogenicity of trypanosomatid species, the causative agents of trypanosomiasis and leishmaniasis, are dependent on a flagellum that contains an axoneme of dynein-bound doublet ...The movement and pathogenicity of trypanosomatid species, the causative agents of trypanosomiasis and leishmaniasis, are dependent on a flagellum that contains an axoneme of dynein-bound doublet microtubules (DMTs). In this work, we present cryo-electron microscopy structures of DMTs from two trypanosomatid species, and , at resolutions up to 2.7 angstrom. The structures revealed 27 trypanosomatid-specific microtubule inner proteins, a specialized dynein-docking complex, and the presence of paralogous proteins that enable higher-order periodicities or proximal-distal patterning. Leveraging the genetic tractability of trypanosomatid species, we quantified the location and contribution of each structure-identified protein to swimming behavior. Our study shows that proper B-tubule closure is critical for flagellar motility, exemplifying how integrating structural identification with systematic gene deletion can dissect individual protein contributions to flagellar motility. |
 External links | Science / PubMed:40080577 |
| Methods | EM (single particle) |
| Resolution | 2.8 - 3.6 Å |
| Structure data |  EMDB-47607: Leishmania tarentolae 48 nm doublet microtubule consensus map  EMDB-47608: Leishmania tarentolae 48 nm doublet microtubule protofilament 1.1 focused refinement  EMDB-47609: Leishmania tarentolae 48 nm doublet microtubule protofilament 1.2 focused refinement  EMDB-47610: Leishmania tarentolae 48 nm doublet microtubule protofilament 1.2 focused refinement  EMDB-47612: Leishmania tarentolae 48 nm doublet microtubule protofilament 2.1 focused refinement  EMDB-47613: Leishmania tarentolae 48 nm doublet microtubule protofilament 2.2 focused refinement  EMDB-47614: Leishmania tarentolae 48 nm doublet microtubule protofilament 2.3 focused refinement  EMDB-47615: Leishmania tarentolae 48 nm doublet microtubule protofilament 3.1 focused refinement  EMDB-47616: Leishmania tarentolae 48 nm doublet microtubule protofilament 3.2 focused refinement  EMDB-47618: Leishmania tarentolae 48 nm doublet microtubule protofilament 3.3 focused refinement  EMDB-47619: Leishmania tarentolae 48 nm doublet microtubule protofilament 4.1 focused refinement  EMDB-47620: Leishmania tarentolae 48 nm doublet microtubule protofilament 4.2 focused refinement  EMDB-47622: Leishmania tarentolae 48 nm doublet microtubule protofilament 4.3 focused refinement  EMDB-47624: Leishmania tarentolae 48 nm doublet microtubule protofilament 5.1 focused refinement  EMDB-47627: Leishmania tarentolae 48 nm doublet microtubule protofilament 5.2 focused refinement  EMDB-47630: Leishmania tarentolae 48 nm doublet microtubule protofilament 5.3 focused refinement  EMDB-47633: Leishmania tarentolae 48 nm doublet microtubule protofilament 6.1 focused refinement  EMDB-47635: Leishmania tarentolae 48 nm doublet microtubule protofilament 6.2 focused refinement  EMDB-47636: Leishmania tarentolae 48 nm doublet microtubule protofilament 6.3 focused refinement  EMDB-47638: Leishmania tarentolae 48 nm doublet microtubule protofilament 7.1 focused refinement  EMDB-47639: Leishmania tarentolae 48 nm doublet microtubule protofilament 7.2 focused refinement  EMDB-47640: Leishmania tarentolae 48 nm doublet microtubule protofilament 8.1 focused refinement  EMDB-47641: Leishmania tarentolae 48 nm doublet microtubule protofilament 8.2 focused refinement  EMDB-47642: Leishmania tarentolae 48 nm doublet microtubule protofilament 8.3 focused refinement  EMDB-47643: Leishmania tarentolae 48 nm doublet microtubule protofilament 9.1 focused refinement  EMDB-47644: Leishmania tarentolae 48 nm doublet microtubule protofilament 9.2 focused refinement  EMDB-47645: Leishmania tarentolae 48 nm doublet microtubule protofilament 9.3 focused refinement  EMDB-47646: Leishmania tarentolae 48 nm doublet microtubule protofilament 10.1 focused refinement  EMDB-47647: Leishmania tarentolae 48 nm doublet microtubule protofilament 10.2 focused refinement  EMDB-47648: Leishmania tarentolae 48 nm doublet microtubule protofilament 10.3 focused refinement  EMDB-47649: Leishmania tarentolae 48 nm doublet microtubule protofilament ODADC1 focused refinement  EMDB-47650: Leishmania tarentolae 48 nm doublet microtubule protofilament ODADC2 focused refinement  EMDB-47651: Leishmania tarentolae 48 nm doublet microtubule protofilament 7.3 focused refinement EMDB-47661, PDB-9e78:  EMDB-47680: Crithidia fasciculata doublet microtubule - ponticulus at position 1 within 48-nm repeat length  EMDB-47681: Crithidia fasciculata doublet microtubule - ponticulus at position 2 within 48-nm repeat length  EMDB-47682: Crithidia fasciculata doublet microtubule - ponticulus at position 3 within 48-nm repeat length  EMDB-47683: Crithidia fasciculata doublet microtubule - ODA-DC  EMDB-47684: Crithidia fasciculata doublet microtubule (48-nm periodicity)  EMDB-48302: Crithidia fasciculata doublet microtubule (consensus map, 48-nm periodicity) |
| Chemicals |  ChemComp-GDP:  ChemComp-MG:  ChemComp-GTP:  ChemComp-ZN:  ChemComp-CA:  ChemComp-ATP: |
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 Keywords | STRUCTURAL PROTEIN / flagella / parasite / axoneme / doublet microtubule |
leishmania tarentolae (eukaryote)
