EMDB-47661: 48-nm repeat of the Leishmania tarentolae doublet microtubule

Basic information

Entry

Database: EMDB / ID: EMD-47661

• Title: 48-nm repeat of the Leishmania tarentolae doublet microtubule

Sample

• Complex: 48-nm structure of the Leishmania tarentolae doublet microtubule
  • Protein or peptide: x 66 types
• Protein or peptide: x 2 types
• Ligand: x 6 types

• Keywords: flagella / parasite / axoneme / doublet microtubule / STRUCTURAL PROTEIN

Function / homology

Function:

cilium-dependent cell motility / outer dynein arm assembly / cilium movement / axonemal microtubule / ciliary tip / ciliary plasm / motile cilium / ciliary rootlet / cyclosporin A binding / mitotic cytokinesis / axoneme / cilium assembly / alpha-tubulin binding / microtubule-based process / cytoplasmic microtubule / Hsp70 protein binding / peptidyl-prolyl cis-trans isomerase activity / mitotic spindle organization / meiotic cell cycle / peptidylprolyl isomerase / Hsp90 protein binding / structural constituent of cytoskeleton / mitotic spindle / kinase activity / protein folding / microtubule / cytoskeleton / hydrolase activity / calmodulin binding / ciliary basal body / cilium / ribosome / GTPase activity / centrosome / calcium ion binding / GTP binding / metal ion binding / nucleus / membrane / cytoplasm

Domain/homology:

Cilia- and flagella-associated protein 97 / Uncharacterized 22kDa protein in aldolase locus / : / Echinoderm microtubule-associated protein first beta-propeller / Hemingway/CFA97 / : / Hemingway/CFA97 / CCDC81, HU domain 1 / Protein of unknown function DUF4586 / Outer dynein arm-docking complex subunit 3 / CCDC81, HU domain 2 / CCDC81 eukaryotic HU domain 1 / Cilia-and flagella-associated protein 96 / CCDC81 eukaryotic HU domain 2 / Sperm-tail PG-rich repeat / Sperm-tail PG-rich repeat / : / : / : / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / EF hand / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / EF-hand domain pair / : / Mir domain superfamily / Quinoprotein alcohol dehydrogenase-like superfamily / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain profile. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD / Cyclophilin-like domain superfamily / FKBP-type peptidyl-prolyl cis-trans isomerase domain profile. / FKBP-type peptidyl-prolyl cis-trans isomerase / FKBP-type peptidyl-prolyl cis-trans isomerase domain / Peptidyl-prolyl cis-trans isomerase domain superfamily / Alpha tubulin / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Armadillo-type fold / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily

Component:

Enkurin domain-containing protein / Kinesin / EF-hand domain-containing protein / Uncharacterized protein / EF-hand domain-containing family member C2 / Cilia- and flagella-associated protein 45 / Tubulin alpha chain / Uncharacterized protein / HMG box domain-containing protein / EF-hand domain-containing protein / Trichohyalin-plectin-homology domain-containing protein / Uncharacterized protein / Cyclophilin, putative / Uncharacterized protein / Axonemal dynein intermediate chain protein / Calcyphosin / Enkurin domain-containing protein / CFA20 domain-containing protein / Cilia-and flagella-associated protein 96 / Flagellar protofilament ribbon protein-like protein / Cilia- and flagella-associated protein 53 / EF-hand domain-containing protein / Enkurin domain-containing protein / peptidylprolyl isomerase / DM10 domain-containing protein / Uncharacterized protein / Cilia- and flagella-associated protein 52 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / EF-hand domain-containing family member C2 / Uncharacterized protein / Calpain-like cysteine peptidase, putative / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Calcium-binding protein, putative / Sperm-tail PG-rich repeat / Calcium-binding protein, putative / DM10 domain-containing protein / Meiosis-specific nuclear structural protein 1 / Uncharacterized protein / Nucleoside diphosphate kinase, putative / Uncharacterized protein / Uncharacterized protein / EF-hand domain-containing protein / Uncharacterized protein / CCDC81 HU domain-containing protein / Flagella associated protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Tubulin beta chain / Flagellar protofilament ribbon protein-like protein / Rib72 protein-like protein / EF-hand domain-containing protein

• Biological species: Leishmania tarentolae (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 2.9 Å
• Authors: Doran MH / Ren P / Hoog JL / Brown A

Funding support

United States, 3 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R01GM141109	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R01GM143183	United States
National Science Foundation (NSF, United States)	MCB-2434879	United States

• Citation: Journal: Science / Year: 2025; Title: Evolutionary adaptations of doublet microtubules in trypanosomatid parasites.; Authors: Matthew H Doran / Qingwei Niu / Jianwei Zeng / Tom Beneke / James Smith / Peter Ren / Sophia Fochler / Adrian Coscia / Johanna L Höög / Shimi Meleppattu / Polina V Lishko / Richard J Wheeler / Eva Gluenz / Rui Zhang / Alan Brown / ; Abstract: The movement and pathogenicity of trypanosomatid species, the causative agents of trypanosomiasis and leishmaniasis, are dependent on a flagellum that contains an axoneme of dynein-bound doublet microtubules (DMTs). In this work, we present cryo-electron microscopy structures of DMTs from two trypanosomatid species, and , at resolutions up to 2.7 angstrom. The structures revealed 27 trypanosomatid-specific microtubule inner proteins, a specialized dynein-docking complex, and the presence of paralogous proteins that enable higher-order periodicities or proximal-distal patterning. Leveraging the genetic tractability of trypanosomatid species, we quantified the location and contribution of each structure-identified protein to swimming behavior. Our study shows that proper B-tubule closure is critical for flagellar motility, exemplifying how integrating structural identification with systematic gene deletion can dissect individual protein contributions to flagellar motility.

History

Deposition	Nov 1, 2024	-
Header (metadata) release	Mar 12, 2025	-
Map release	Mar 12, 2025	-
Update	Mar 26, 2025	-
Current status	Mar 26, 2025	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_47661.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.33 Å

Density

Contour Level	By AUTHOR: 0.0435
Minimum - Maximum	-0.011680627 - 1.8413265
Average (Standard dev.)	0.009867804 (±0.05819203)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 680.96 Å α=β=γ: 90.0 °

Supplemental data

Half map: #2

• File: emd_47661_half_map_1.map

Half map: #1

• File: emd_47661_half_map_2.map

Sample components

Entire : 48-nm structure of the Leishmania tarentolae doublet microtubule

• Entire: Name: 48-nm structure of the Leishmania tarentolae doublet microtubule

Components

• Complex: 48-nm structure of the Leishmania tarentolae doublet microtubule
  • Protein or peptide: Tubulin beta chain
  • Protein or peptide: Tubulin alpha chain
  • Protein or peptide: CFAP20
  • Protein or peptide: PACRGA
  • Protein or peptide: PACRGB
  • Protein or peptide: CCDC81
  • Protein or peptide: CFAP21
  • Protein or peptide: CFAP45
  • Protein or peptide: CFAP52
  • Protein or peptide: CFAP53
  • Protein or peptide: CFAP67A
  • Protein or peptide: CFAP67B
  • Protein or peptide: CFAP106A
  • Protein or peptide: CFAP106B
  • Protein or peptide: CFAP107
  • Protein or peptide: CFAP115
  • Protein or peptide: CFAP127
  • Protein or peptide: CFAP141
  • Protein or peptide: CFAP143
  • Protein or peptide: CFAP161
  • Protein or peptide: CFAP210
  • Protein or peptide: DMIP5
  • Protein or peptide: RIB43
  • Protein or peptide: RIB72A
  • Protein or peptide: RIB72B
  • Protein or peptide: RIB72C
  • Protein or peptide: RIB72D
  • Protein or peptide: DMIP1
  • Protein or peptide: DMIP2
  • Protein or peptide: DMIP3
  • Protein or peptide: DMIP4
  • Protein or peptide: DMIP6
  • Protein or peptide: DMIP7
  • Protein or peptide: DMIP8
  • Protein or peptide: DMIP9
  • Protein or peptide: DMIP10
  • Protein or peptide: DMIP11
  • Protein or peptide: DMIP12
  • Protein or peptide: DMIP13
  • Protein or peptide: DMIP14
  • Protein or peptide: DMIP15
  • Protein or peptide: DMIP16
  • Protein or peptide: DMIP17
  • Protein or peptide: DMIP18
  • Protein or peptide: DMIP19
  • Protein or peptide: DMIP20
  • Protein or peptide: DMIP21
  • Protein or peptide: DMIP22
  • Protein or peptide: DMIP23
  • Protein or peptide: DMIP25
  • Protein or peptide: DMIP26
  • Protein or peptide: DMIP27
  • Protein or peptide: ArcMAP2
  • Protein or peptide: ArcMAP1
  • Protein or peptide: ArcMAP4
  • Protein or peptide: ArcMAP3
  • Protein or peptide: CFAP96
  • Protein or peptide: CFAP97
  • Protein or peptide: DMAP1
  • Protein or peptide: DMAP2
  • Protein or peptide: DMAP7
  • Protein or peptide: distal DC1
  • Protein or peptide: distal DC2
  • Protein or peptide: distal DC4
  • Protein or peptide: DC5
  • Protein or peptide: DC6
• Protein or peptide: DMIP24
• Protein or peptide: DMIP24
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: ZINC ION
• Ligand: CALCIUM ION
• Ligand: ADENOSINE-5'-TRIPHOSPHATE

Supramolecule #1: 48-nm structure of the Leishmania tarentolae doublet microtubule

• Supramolecule: Name: 48-nm structure of the Leishmania tarentolae doublet microtubule; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#49, #52-#68
• Source (natural): Organism: Leishmania tarentolae (eukaryote) / Strain: P10 / Organelle: Flagella

Macromolecule #1: Tubulin beta chain

• Macromolecule: Name: Tubulin beta chain / type: protein_or_peptide / ID: 1 / Number of copies: 162 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 49.830824 KDa

Sequence

String:

MREIVSCQAG QCGNQIGSKF WEVISDEHGV DPTGTYQGDS DLQLERINVY FDESSGGRYV PRAVLMDLEP GTMDSVRAGP YGQLFRPDN FIFGQSGAGN NWAKGHYTEG AELIDSVLDV CRKEAESCDC LQGFQLSHSL GGGTGSGMGT LLISKLREEY P DRIMMTFS VIPSPRVSDT VVEPYNTTLS VHQLVENSDE SMCIDNEALY DICFRTLKLT TPTFGDLNHL VAAVMSGVTC CL RFPGQLN SDLRKLAVNL VPFPRLHFFM MGFAPLTSRG SQQYRGLSVA ELTQQMFDAK NMMQAADPRH GRYLTASALF RGR MSTKEV DEQMLNVQNK NSSYFIEWIP NNIKSSICDI PPKGLKMSVT FIGNNTCIQE MFRRVGEQFT GMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATVEEEGE YDEEEEAY

UniProtKB: Tubulin beta chain

Macromolecule #2: Tubulin alpha chain

• Macromolecule: Name: Tubulin alpha chain / type: protein_or_peptide / ID: 2 / Number of copies: 158 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 49.811133 KDa

Sequence

String:

MREAICIHIG QAGCQVGNAC WELFCLEHGI QPDGSMPSDK CIGVEDDAFN TFFSETGAGK HVPRCLFLDL EPTVVDEVRT GTYRQLFNP EQLVSGKEDA ANNYARGHYT IGKEIVDLAL DRIRKLADNC TGLQGFMVFH AVGGGTGSGL GALLLERLSV D YGKKSKLG YTVYPSPQVS TAVVEPYNCV LSTHSLLEHT DVATMLDNEA IYDLTRRSLD IERPSYTNVN RLIGQVVSSL TA SLRFDGA LNVDLTEFQT NLVPYPRIHF VLTSYAPVVS AEKAYHEQLS VADITNSVFE PAGMLTKCDP RHGKYMSCCL MYR GDVVPK DVNAAIATIK TKRTIQFVDW CPTGFKCGIN YQPPTVVPGG DLAKVQRAVC MIANSTAIAE VFARIDHKFD LMYS KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GAESADDMGE EDVEEY

UniProtKB: Tubulin alpha chain

Macromolecule #3: CFAP20

• Macromolecule: Name: CFAP20 / type: protein_or_peptide / ID: 3 / Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 24.686338 KDa

Sequence

String:

MFHNSYQAGF LSILYSIGSK PLQIWDSQVR NGHVKRVTDE DIQSSVLEIM GSNVSTCYIV CPANPKQTLG IKLPFLVMII KNMKKYFSF EVTILDDKNM RRRFRASNYQ STTKVKPFIC TMPMRLDEGW NQIQFNLSDF TRRAYGTNYV ETLRVQVHAN C RIRRLYFS DRLYAEEELP PEFKLFLPIT QQPPAAAGEQ RDVEAADVPQ AASVAQ

UniProtKB: CFA20 domain-containing protein

Macromolecule #4: PACRGA

• Macromolecule: Name: PACRGA / type: protein_or_peptide / ID: 4 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 34.208086 KDa

Sequence

String:

MSSYEIKPIV KGTKRDPSLL KYNKAAGAGP FGAAPASLRT KKSAPDQSAA APPSPGGAAN TPVNAVRFPY TGTTHGYGGA CSSLRKGRP RDAPDAAFSE KGCGKSAPPK AGAFKKRVIP PTEFRRAYNR GDLPIAICHG SRPTVDWKVE VEKLDYHHYL P IFFDGIRE TEEPYMFLAR QGCLDLLERG GSKILPTIPQ LIIPIKTALN TRHPDIISAT LRILQHLIVS DDLIGEALVP YY RQILPVL NLFKNVHKRR SRGDAMDYGQ RNRDDVGDLV NETLQLLEQH GGDDAYINIK YMVPSYESCI YNN

UniProtKB: Uncharacterized protein

Macromolecule #5: PACRGB

• Macromolecule: Name: PACRGB / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 34.656672 KDa

Sequence

String:

MPSCKTFSQL EREKLRKDRD VLPPLSEGPF GPFPKNWHQK ERDASKATVS TSASASMAKK NSAAEKPAAA AAPKAAQASI LIGHTGTGG MAVSTFKGTG CAITSLSGGR FGDPAGPGKA GAYSKQRVPP TDFRHHYERS DLPLSIQHSA KRSLLWKVEV S KLDYHHYL PIFFDGLREL EEPFSFLAYQ GSMDLLEGGG AKILPTVPQL IMPLKTALNS RHPDVLRKVF HVIQKLVVSG DH IGEALVP YYRQLLPVFN LFMNRGKSKG DCKDHNQRNS NDLSSIINDT LYLLERYGGP DAYINIKYMV PTYESCM

UniProtKB: Uncharacterized protein

Macromolecule #6: CCDC81

• Macromolecule: Name: CCDC81 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 29.507893 KDa

Sequence

String:

MEQSSSCSGR VCRGCYGEIA EVVSHMNGVY MLQTKGQGTA HQLNAIWRVL GEQLEEMLIK KRSGIVLDFL HASIKVQRIK RFDNSIALK LKPQFVLVPD FTSKFHLKNV LEMQDAHYHA TVPNTVSYIT IASIVGTDRF VVEAAVKDSV REIGKYLQRN A ASTLTIDI GVGFVEFKDR TYRMKWSPEF LARMKASVGT DGVVTPYDPP SRTIGGPTAP CRFQKGCTSE NLLQTQVRDT ML AESRLTA AELNDGMGGS SYRRTHW

UniProtKB: CCDC81 HU domain-containing protein

Macromolecule #7: CFAP21

• Macromolecule: Name: CFAP21 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 44.077684 KDa

Sequence

String:

MSTYANSQAR LNRVAPQLRP AGIHGDWTEA TTAELLSSYN PNGVTTPDHI RSFHHRGLDV GEQRRHWGSA KDAPVDPDMR HGVKGKETG GADACLRPEM YADKMTALLD AQRETQYLSN RRKPLGHAPV PRDPVPVPFC GFGVTQKKGD STQSVMAGYR S VDVLHPVG EQLTRNYDWE SAGIDPTQYR FGKRSTSSDG TTATALCSDS ATQLTSKVAK DYGTIVAKEL GQSKNYGFDD PT EWDEEKR GTVTKFGTTG TTASYFQTEQ PTVRELLSSW AQTASGSTTN KAASGKADQQ GLSDSANATE ADKADTSVAA PVV SGLSLA GPLKDDVHAH QLLYPCHYVS LGVESKYFAG GRPVEDIRQL CHKCDFGISD ADIDTVFALV AKGGSTCSIE EFKN AARAK GYM

UniProtKB: Flagella associated protein

Macromolecule #8: CFAP45

• Macromolecule: Name: CFAP45 / type: protein_or_peptide / ID: 8 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 57.008625 KDa

Sequence

String:

MAFFFTKTCD VFPPRRRGQS DGALRKELNA RGAPRDSAII TKTELDIIRG MIDGHFPGSL GSTAATAGAP RTHTEAAEEH RRRMQEFDA DRARNGVAPR TAEEIEEAQL RQLNLEKARL MLDEDCDEAK AMNRVIMEAK CIATREAQRL EKQKRAEEEM E YNRQMDAL MAQEAETAQK VYLERERQRM EEQQRNASMI KTQLHERYVE RVRRLERHQQ EQDAMSRHIE RLQMEEKAEK LR RIDAARR LMEEAAIANA EQISLKQRER EMEIEEERKM AEYIKKKEAR DEAYAEEQAR IRREKDMEIA RLRANQQRAQ NKE AELEEL RARRVQEAYV REERRKEKEA AERESAMHAD LQKARLAQIE ERKRQKALEK VQEQEELDRL LAVQKISREQ ELER QARAR RLQEENSLAL LKQIMDVEER RRRQRQEEIE EGNQIRMAER ERQAALEVIR DRKLGELEEL GVPDQFRQAL LKVGK

UniProtKB: Cilia- and flagella-associated protein 45

Macromolecule #9: CFAP52

• Macromolecule: Name: CFAP52 / type: protein_or_peptide / ID: 9 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 68.550664 KDa

Sequence

String:

MTEHENTPRL ELETALGYSG NLPGSLVAHP DQKHLLYALG ACIVIRDAND AESSEFFYGH NDKISCLAVS VSGRYVASGQ VTHPGFQAD VCIFDFAERR LIHRMLLHKV KVQALAFSPD EQYLASVGGP DDNTVVLWDV KTGRPLCGAP AHHTETKAVA F FNNHSEKL ITGGVGSLRV WTVDLEQRKM NPVDINMGNM RRSVQTISVE KTDKYIYCGT TSGDVICAQL QQANVFKMQG PQ KKLSGGI LSTILTHTGD VLVGSGAGEV QLLSKINLTV QNSTTVKGGV TALALMGDNY YVGTKTSNLY FVNGGNFMVR LRL TCHSEA VQDIVFPHGF SSVFATCCDT DIRVWNANSC TELLRIEVAN LTCNCLQFSR DGSLIISGWS DGRIRAFGPQ SGKL VFSVA DAHKVEQGSR SHKVGGKVGV TSVCADNTGH RIVTGGSDGL VRVWAIRGAT CTLEASMKEH KAAINAIVIS HDDTE CVTA SDDGSCIVWD LTRYLRRNIM YRQTYFRALE YYVDDSQLIT CGSDKCIVYW DSVDCHPIRE VCGSRTAELN SLSLSP DGR FFVTGGNDRI VKVWDYDRGE CIAVGLAHSC SITKVRVSPD GKKIVSVGDE GAVMIWNIGE LAIEGL

UniProtKB: Cilia- and flagella-associated protein 52

Macromolecule #10: CFAP53

• Macromolecule: Name: CFAP53 / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 59.240293 KDa

Sequence

String:

MPRSNVGVSS IEARQRRERF MEKFNAEQTV QERQKRTVEW ELRGNERHAQ QEVLVYMDRI QAQHNDVLVA RRRRLAELLT RENELHTSM MTSLPETDAQ RRERLIRKAQ ELRAKREEAK RLDISARHDR LFCAKIDCLR QAESRLKVMQ VADARYEQMD A AAERRRQE AAEDLLYAQQ NAEAQRVATE RVQRDLEEQY NRKKRMIADL EVQVEGNRRR KEIEKENARR DQEEFYRLLH EE QAEEARK RLQRQEKNRQ LAQEMIEMNE ELKRARQQEY EQLRREDKEA LDAILASLAA EKQEQLAEKK RRMAEERQHM LEL QKMAKS KANTDAIEKL WAEENEKQWR KREAQWAADQ AKRDALLRNI LIARRQQILD KRQKDKEDAM LRKLEDEKFL ESLA KERDV DAAERQRRMA LLKETQQYLE WQIRQRIAEK EAAKLAKRTE LTDEQALEKQ YEDRIAREMA NLEAAKPERY RDVPL LPKK HRNQLF

UniProtKB: Cilia- and flagella-associated protein 53

Macromolecule #11: CFAP67A

• Macromolecule: Name: CFAP67A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 37.086184 KDa

Sequence

String:

MVGEQRDTFV VEYFDPQASL SRTYQFCYFT EDKTIEMYDL KTKRLFLKRC AYPSLSPNDL YVGATINVFS RPLRIVDYGD DATRKRLTG NSGECMIAVD MQHHSAAAGS VIEALTTQGL RITFIRLVEL SQSLATRVAS KSQRCLVLLA SGAEAREKVA S VAASFSAA VTQISSESAV QELKEVIMGP GESTATLKNC AVCVIKPHAI TSGHQGPILH RLVEEGFYIS ALGSYQLTVA DA EDFLEVY SGVLPEYKKL VEQMSSGPCW AIEVCAENAV PALRAVCGPH DPEVCHVLFP HSLRSMYGVD PIRNAVHCTD LEE DGPLES EFFFSLLQNK Q

UniProtKB: Nucleoside diphosphate kinase, putative

Macromolecule #12: CFAP67B

• Macromolecule: Name: CFAP67B / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 37.469871 KDa

Sequence

String:

MATSTLDSRM AFHAQQQDPG APQPRQLILR YFYESGTVEL MEVPSGRLYL KRTAVDIPAS SFTVGSTVML FGKATTITAF ADEVTRQLC AQCSESTTVV ITEEAFPSLG RYLAMLTEEC CFTITDVEMV WVQRETISSF NLPEKLADTR IVVVLCTRKK A VEKGFAFV ERTTGTCTAK DAEQAALWGY LAQLAKAKPL AVFNEVNSSV VVLKPHVVSS GCGGSICQKL LDVGLEPTAL TT VTMTSAA ALEFMEPYRG VLPNLEGTVS SFVGTNWVLQ LVSLDETVDV VDTVRKICGP YDTVIARKLY PMSIRACYGD SET NNAVHC CDLPSDGPVY TKFFFQG

UniProtKB: DM10 domain-containing protein

Macromolecule #13: CFAP106A

• Macromolecule: Name: CFAP106A / type: protein_or_peptide / ID: 13 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 30.917178 KDa

Sequence

String:

MSGECVYDLV PANDSVGVGG RVVTAKPAAA VKKSRRAPPP TASTFGIHGT SAVVANAGGE YTDASVHPSR KPIGTFGREV GSTVTPEIF LRKNEGPLTA SRGAPTVNDS MFKKSEYHRE KTKPDVPLRN EHPVMGLRTE KNFVVANAVE NTMAVPTKVI P AAQPRATD REDFGKVPKY IKEIKEDINV RKQMLKDNET AGRVAQELWS EISPSELAQL IDGLQRRWEA LNKDYQKKGF GK LETPSQR AHQELVEKQL VAVEFAMQKL RRHHVFVFDD QK

UniProtKB: Enkurin domain-containing protein

Macromolecule #14: CFAP106B

• Macromolecule: Name: CFAP106B / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 41.609734 KDa

Sequence

String:

MFSEMQEIVA TAHQLEFKMS GESIFGLYAK SAAEQKLLTK SESPYTGKYD KHMAEPGKPS YSTFFEKAKE YDGTNVRFFK QREAVIGKN VGDTVDPQKY LKKGDGIRYI VPATHEEKVY RKPPVDNSTP FSQGLKNGDA KSTPAGIQYD GDGNPISNGN G FLGAGSTG QDQSGAEDSG NDGAAHGYGI GDYRADDRSR DTFSQDATKN FVASNIVEIS NMVPKRRKMQ APLPTSRKSF GE TPAYIPR VKREISEEKA FLESLQEAKV ERQKQVHAKY IYLLPREEQD KLVQSMRKRN DECICELQRM PFSKDTAVMR KRK TELEKT VADIEVALRK LDKDALFIYK DDPVNGQWCK EAALKEAQRY AAHS

UniProtKB: Enkurin domain-containing protein

Macromolecule #15: CFAP107

• Macromolecule: Name: CFAP107 / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 30.415637 KDa

Sequence

String:

MATLATGEQS VKSAYHCATL LDNWAEDRHQ FGQPVPTTSH ADGFDPNTTY SASYKSLTEA QIAEAKPPGC FAAEAPRMLL FHHGDIGNI ETYCYATSEL AHTDRKKQVY TNDEVLDMPG VSTRRNKTAA LQRSTMGFST SGSGHAGTST NGLRADEVSA A VQREAAYL QSMASTMRST QHWANGTQDS TRDPGSETTE TKTGSGYAPP RLLPMGRVAE RERLLTTKNV SIDASGVYLF DN LEAYPLT SSQCVGKMTK WCDNPMHKTN LRIHYMEDDY

UniProtKB: Uncharacterized protein

Macromolecule #16: CFAP115

• Macromolecule: Name: CFAP115 / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 105.84607 KDa

Sequence

String:

MSSSVPSFDA AMRKARAQVG KRRIFLKSFF ADFDRLRCGR ITAAQFARVL TNNDVHLSPE EMRALSRRFA PAVAAGTSAA AARAAANAS GKDDAEVLYE DFLAALEAEK GSSASVRRST PSVALTPAEE ARLKPFQTML RQAIQAHGTS LTAPLRDLDP L RTGRVTVA QFRRCLPFSS SLTEDAMDLL AKQYSDGHGG VYYMAWCRAM DPSLNGQGES ESALGAADSA RQRAIDKDQS NQ WKTGSVS SLVCPDSFTN PRLSAEELIV VFRQQCALYR LRYEDAFADF DKMKTGKVTV AQFESVLGRM PLVHFALRPE NID TLARAY ISTSSSSSNG VPVNGTPSGP VVEYRAFLHD INPAKALETT ADGSAVATNF FATTHAADTY LTSSDEQRKA EALL SHLRA LVQSNRICLS PVLRDFDRVR KGIYEHRTCT RTRFARGLAT QNIMLPPEQL QLLIRKYTVP NPDGSPSSEV NYYLF VQDV DPSQASGCKD SNSDGMRRTT GVVVPPFTGA ASAPATAVAS VTSLTKENVL ANVALQVVER RLHVAAFFAD ADPLHS GTI PKERLGVALG QAGLQLLPEA LAVLQSAFAS TRIRDGVDAQ KLATEVEEAV AVLRARRTAE KTGGTGDEST AQDAERA AQ VAAILSRVRH NVSVHNALLM PFFADFDRHH RGVITSSQFA QACVRHRLPL TETEMHTLAS WYSAGDAVTP GTDGVRYL S FVRDVGCEEE SVQYALAQKA SATGGTVDTS LSASARKAIE AAADVDEVLT DICVFLQERR PCVSEFFPDG DELRHHHVT PSRFRHCITM LGLTDMTEAQ LSALEGAFAS AKCPGDIDYP AFVYTVRAML ADGAGAAAVS QRRLQGSTSS GRGNGDVTGD SAPTQMTAA QGFAAATLQH IQRTLKARRT ATIAAFREYD RARKGYVTEG QFFACLQALG VPLKPDEAAA LLQLYAVGNG Q VHYIAFAH KVDDDSFITV S

UniProtKB: EF-hand domain-containing protein

Macromolecule #17: CFAP127

• Macromolecule: Name: CFAP127 / type: protein_or_peptide / ID: 17 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 53.012078 KDa

Sequence

String:

MSSSEARAER LRRVNRYKAI QAELARQKEE SDFAAMRAAK VKAAATDEAL AAALAEQQRL EVKDAKMLQL VSDYPEVRSL ESQLKDAYL RKSRAEQVTD RNAAKALEVE EQRKYMEYLA GQDRLAKEEE EKKHKEEMER FQRHQAAQLE IIRQHRCAAM E EATRREQE RIAVDAVVAR VQEQDFLENL ARRDRQRRIM EEQDEFCRLR AAIKKAEQDR EAKEEAAIRA YLDEQARRKE ID DKTARER DIVKARILEE QGRRIAEEEA KKRELEALLQ EYYEEERLTK EQMLIAADKA SRDRMAAAVN RENQKLIEQR RLA KEEQLA EELRFRQEAM EQMAAEAQLQ RLNRQKQAEI KRQHIAEAQK RLEERRRLKE QEKELERKVE EKDRKQEEQI QEYI RRARA QLLAQHLPKL GSYAPVFAMR DEEKTQFLPH LKNSNATLPK H

UniProtKB: Meiosis-specific nuclear structural protein 1

Macromolecule #18: CFAP141

• Macromolecule: Name: CFAP141 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 34.252605 KDa

Sequence

String:

MVRPRTGAHP SVCLAPFRAL CVRACVCVCV CVCVRQKGEQ RRLCILWRPH THAHGRLCAA SSHAPTLPLL STSCRRSSLS LLCPLSLFA APLGFERAVP VDWDAPSSPP PSLVRRRAGT PPLPRRRRRV LVGPPSHTIL RELHSAPPPA AAPLFVSVNS G TVLRKPMR YYPSGDSRES YITGIRPGGR YGAMHDVEKR RDNLPDNVFH YMKDAVQREE LLRLNEEEER ARIRLEMFAK KP GRRWGNA STDAAGTSGS AEQSVAISKL EYETVTRERR TALQGLYQRE LREWEVQLRA RGLATQRT

UniProtKB: Enkurin domain-containing protein

Macromolecule #19: CFAP143

• Macromolecule: Name: CFAP143 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 34.200926 KDa

Sequence

String:

MMDPYYGKAR KPYVPIDTCS PGGTWVEERR YNRVRPTVTG AAAASMEADA SGASSPNADT LTAADRRPRR VLCGNWQEED ALERDMLAI QEAQLQQTQS EAGNEPPASS SAPVQHMQRF LQSGWNPTAT MEVITAKPNG FYSGGHTSTP YLTCDSRHPT M RNLDSTYR VDFNHTSSNV ASLRRPELGV RSQRLMEQVR VAAKAMQDAN LRTRQEQQQL REGSMKGMSR SYGPNVSEYR AT MCDENEV LPVVEELSQD YLSDEPITLY TGNPNTGKTM TVHGKTPVDP MSSSRFGKHT HFSEFKYAY

UniProtKB: Kinesin

Macromolecule #20: CFAP161

• Macromolecule: Name: CFAP161 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 32.782809 KDa

Sequence

String:

MPRKALLGNW FEEEAYERDR QRLMQGRNGG IVEASAEVAH MMAKIRHHNA PHSIAPIAED GYLRFYVPLM LQNAHTCGFL SVDLDDRKA TPTGWQVECS TAPAEEATSR CTVVLTPAAM PQTDSFPIPE DEADIVHYGQ PFYLMTVREL CEDPLFLMSE F ITPGCASP VTQKLQHTYF SPDGGSAEAM WCIEDANPAF QEDMRDHPVK ADDVIRIRHN MTAAPLASLR EVFYNDFGAQ FE LGCGRLT TLATKRRGGP PALENLWMFI HDGQGRQQEP DEGAVHEGAA TQEASAAP

UniProtKB: Calpain-like cysteine peptidase, putative

Macromolecule #21: CFAP210

• Macromolecule: Name: CFAP210 / type: protein_or_peptide / ID: 21 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 62.177062 KDa

Sequence

String:

MPKPRGTVAV EATATATSMS STRARKDPLR RIGNGNAVAG GLDMTRVGIL SDRELAFFQR LAYTGSQAAE QQQRDARAQE EERRRALSK ARAAGWTDTI EARHDQFLQA QQDAKEAAEA RQKVLDELYA KQLEEQHNAV VARRDLEQLK DDPRGRHLHS M QMLHNALT ARKEQVAYKQ MLKREEEAQN ANDQREFQLQ LWGDQAEELH KKLRARQRNV EEKNANLETV LYQIDRRQRE RE DQKQDRK HVEQEAAEER AEQQEEEAQR RARELENGAY NKAHSRPSLT KSQKLQTRVA ESVKDEAALR AEEEKVDSIK RWV MERQKK KQAAFDERKE VGLQRYSEEG KQENLPKYRT QDVFEQKGQS FLQKLYDSNA RQEEKNREYR LEMEQQRREM EEQK AAEAR GDDVGSGDGA NESSRRRTAA PSAAGFLTKA EEKAYVEEMR RYPEQLRAKE AAEAAARRAE ALRIEHIQKL QAAEK REKE RRAVEARREE FQQQQAQQEA DDMRYRAYID SVVPADMDPV LYRKAIQLH

UniProtKB: Trichohyalin-plectin-homology domain-containing protein

Macromolecule #22: DMIP5

• Macromolecule: Name: DMIP5 / type: protein_or_peptide / ID: 22 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 31.664545 KDa

Sequence

String:

MSLTASTLPP VKGTSSKKSL QKGRPADSKG NGTAPAANPH CYHKCRCYGH DDTLPNYKLL TIPRTNNYYA ATGGCPCVPR VAAHPGSWR LKEGQLRSST YRCGTGCIKK SAALTCRCCH HSGDQYGWRR GPHTVLDKNT AATGKCDTFG NLAFKSTSRA Y CAVDGDDL SSVSSASPEK LEDLEALILE EHQQRMRVEE DVDELAAIQR DGTADWYKKS YNDAARRRFL SQISDAELQD LL RDVKYIS DRPLNQTNMD LLRRILHRQE HLQRQREYYA AQEQE

UniProtKB: Uncharacterized protein

Macromolecule #23: RIB43

• Macromolecule: Name: RIB43 / type: protein_or_peptide / ID: 23 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 47.05159 KDa

Sequence

String:

MDSVAEEQDV LTATNSRGVA AASTADNSAS ASNTMNTKTK KQLFMLQRAE RLKDPKMRKM GIDREALDAQ VREKEALRRL EKERNDYYD EQALLMDRHA CALQQEVNSI RAAREKELQD YRQTFQKKEM AREWDLNDPE ARRKELPARV GDDDPRNGPS S LQKFEGED LDYAARKAAQ QRQQRQWAQQ QVNEKLAKKW MEQERDRAFD DRNEEVNYRL YEVEQKVAEQ RRLMEKNGAD FN RALAEQQ RREAVRAKEV DTLLSLQEMA YQMDSDFLNE RETVVSELGA SVKAERYKGM SEKQKALLRA GQDEQLRELR RRR LLEVEE KKQWSLQENM QLRMANALDR QRERERRAER EQLAETQKMQ AEAAAERKAQ LDELYKNAVD EDYFKYWDRC L

UniProtKB: Flagellar protofilament ribbon protein-like protein

Macromolecule #24: RIB72A

• Macromolecule: Name: RIB72A / type: protein_or_peptide / ID: 24 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 85.152797 KDa

Sequence

String:

MAYAQSQKMQ KEFRLPMVPG LSCGEEMMRR NYDRSQIHGV RLDCATVIAG VPADRSLAGG SSAYPGSMLK DAGTAVVAAA GADGESAED LTGRVLRYYG YCIEPVSDSA VETERVRKVT LHFYLEDGTM SVTEAKQDNS GFAFPANLKR HIVPNPDGTP I TAAHLKVG QSVSFYGRTY ELYDVDPFTR ALLKEAGEEV PPPLVPPTDV YTTMRSRPVA KAHDVPSIAA SSPLNTMLSS AQ VRATRQF LEYDRKVLRC DCTWDDTTNL YGTKHFLTLY YFLSDGSIAF VEKDVQNSGR DPFPKFLSRQ RIAKPTSASG KFD SSSLGS VTFKEDANTV YYTAEDIRIG NVLNLYGRQV KIHDYNQYTR DYMAEKFGIT AYAPIPGATP PPFMPPCSTR REIS EDTVR ERHEEKYKEI RRHRFADSVV KFLARLDNGK EEDKVRRFVV AVYLADNSVS IFEPVIRNSG IVGGKFLQRQ KVRRA DGEY FRADDFYVGA RVELNSFPFL ILNSDEHSLN YMEHNPEEFG HSDINKIVRK MQAMLQSSTT GLAEAFRLAD ENPCGG LEM DVFLSIMKEL NLDLTEQEIL TVLRYFDKNN ESYVSYEEVA SRIMPEGGAV ASDNRPWEAI YQEGAEHATS TFLDDPK EA EEKERMRHEN AAAARGAAEF LQLYNQRRQL FMKEFHAITD YAKDSLIGSD EFKMCVRRKL VLSSISDEEM NALAKHLF P AEAPRVPYEE FMRLLNGTST HSHTLVAITS HAASQ

UniProtKB: DM10 domain-containing protein

Macromolecule #25: RIB72B

• Macromolecule: Name: RIB72B / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 88.754805 KDa

Sequence

String:

MKNSVARTQP KKYFAHAPQS ENLPLAIGAD FHDDPINRST LRKSHNLLLE RKTDHAPHTK YCYNGDPFEK SRIAAEAVDA EDEAAVWDG WFSEDFTKED LDDFVVRILG YFTEDVSESS TEKERVRKVL ISFFVKDDSV SITEIGQRNS GLGESKILSR R QVPKDMRN PNDILLLSDF RIGDSVKIYG QTFHIVDMDK RSRRYFRDVL KKDVPDGQSI PQDSFSRAAA ALAERSVKRV IT SDDMDRK RAVEQQLTGI YTKHSTEDIA ITKEFLKNKI NEHLTYLALW DDRGNLSGDL HYCVIRVFLE NNSIEIAECR PEN SGRWGG PILIHRQRIP HPSADLTKSR YQQHTYGKLM KNDYLCPEDI QVGETYMIYG KPFYVYDSDS FTRNYVREKY QVVI RDAID ITPFETPKKH TSVFYPPPPN GFGSERETRS NWLSLVGKPA KMDQEMAQRE SGRVMRFSAK LAKPMVAGDE SREFM VSFH CETKELEIFE KPKRNSGMLG GRFLAKGAHR KDMPNGTTVP FTPDDFQVGR VVEIYRRPFL LTGLDAGTQR ILDGTD KVV TEDRIKYLLV LLKQQLNLQF SRANEAFLAL SSQGTLGYAQ VKEFLRACSC SITDEEALLV VQNLSPGTNG VITYDDF LQ IVDVSSESMD EASLTTRSIR NVDMTKNDQL KLASTASEEK QRRNHLRDVL QFKLIQRKGS VQDQFRLLSD HSANSRLN R NTFRTSLNTV LHFNMDKKDE DTLVSLLFDG IEDENGDITY KQFQEFVDSV DNN

UniProtKB: EF-hand domain-containing family member C2

Macromolecule #26: RIB72C

• Macromolecule: Name: RIB72C / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 84.034211 KDa

Sequence

String:

MSIREKTVYN ALPKLPGFSF AELQHPATHG KRQYCTIDSE GTRTVKDAAA LDGSVVAAIS APEGCGIPVR IILQRFPSWR TMDDKALRF FAYYTEDVVG SRIETWRVRK VKLHYFLADG SLSIVETPAV ANSGLQKGTI VSRFRPDGVD VFRLCIGASI T CRGLEYNL VDCDAFTREF YEVMGMPQPE PLEYPCDEFE TSVMRRPGRV DEEHLQMRRV IEVQAATRSG THASLLTPAE RT KARDFYE YDRNVLRFYA VWEKRKFRIL FYLADGSIAV LFDKAENDGR DPYPIFVSRR RIPKEARAVL LSPETLNQPQ GPP PACVRA EDLRTGTSVD ILTRPFFIYD CDEHTRAYME ARGIPQPAVA VPLCEEDEIT VGKRRPRLTQ AARNAVSDSG KRFG ASTMV FSDSAGEKDA LKLTRYAQDV FRFGAELVNP SEENEGRQFL VCYYLADDTV SVFELQVSNS GHVGGKIFAR RAVEN LQDP ALLKVGNIVR LDGVDYLLKE MDERTKRYLE MGMPNMEESY FRTQEILGVV ASYLQQQFSR ITDAFRHYAS SSTQGL TRE NVREVLSDAG VHATDSELTN VMERVDGDKD GWVSLADFTE QFMHQLFISN FKPRESQKRW GDTRNGVVVQ RGPVQSA MM LAKKADAEAQ AEASLQRFLT LIEARRTLAI RSFRSASESA YDGNLGISEF QDCLKNRLKA PMTDDEIGAL LYRFYHTP G LNDWTARRLP VQEIRRILLL

UniProtKB: Rib72 protein-like protein

Macromolecule #27: RIB72D

• Macromolecule: Name: RIB72D / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 96.057531 KDa

Sequence

String:

MSSDPQLRLS MANQSHVPKE YVRAVDPQLL PPRVGHNWND QAFRSKQGKP QQLEAEFRGT RVFPATRNSN FPKPAPDMCR TAQQLVDAL MSSSLNRNTA TRKARAAEDL CNVTLEDKVL RFYAFFNEPA PEAGAAVFWH RKVVISFFPA DDTILIEEPR I INSGLDGG VFLKRQKVVA DPRQREQFPG EEYVSLNFLN VGGSVRLNAT EFFLYDCDKF TRDFLTVLGV EVGEPVECPD DF FMSEYGR YQERLKSGKL GLLSQDYSAD EVEHSVRFIR DGGKMLRFYG ILDERDSFPG GMVRKLEVML FVEDNSMAIL QRQ SSAESC QGLYLSRCWL PRSGSVAKAN ELTFAHRVNG QREPDMGIPD AYYRDVDLDV GVTLNVFGRS VLLYDCDDYT REFF MERYG VSLRQPIDVS SLFEGDSHRT VLGTEAPTKN FLGDSGGSVG RQPATAADAG DADGGIAVSS ANAAAAALGT LPDTL CFRA VFARPVNHDD EQRRFTVTYF TNSEEFVVHE SAYLKAGIKG GCVWKKNKVM KIPPKPGPRN APKPPAVPGE VPHYTL SDL GEGKEVVING IPLRLYRMDA HTATFYARCA GTLPEDTAGG PAMVTDATGA MVTVDYLISE LRSYLHSRYG TGVASFL SL DRDRDGIVSL PEFTMAMKAF QITEDRGAAA AIFAHIAPAR DTGYFTSVDL MKWMDAVGDG HRESSVRISS MPSGGKEA E LKEIERRALR SKVLRELKSR LDARCWKGAD MFRLASTMPR AYRDRRDDLY SFTNPDRDTV ITPVQLRRCI VEILTGTPT AEEMACLLEF FFPHLPAEAY TQTRDNGTTY AVDLNEFQKR YYVMTKETML PDDDAVAASG S

UniProtKB: EF-hand domain-containing family member C2

Macromolecule #28: DMIP1

• Macromolecule: Name: DMIP1 / type: protein_or_peptide / ID: 28 / Number of copies: 13 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 29.929906 KDa

Sequence

String:

MTQLHSVASL DYGDGRTCVC RICECGKHQC PGLRVPFVGE TTYRDEYVGK KLERERPRKA RQTVFPSKAE PGHFKTTKQE AAELLQGKD LRPAESYKPR EAAAAPLPFE GTTTNRADFP GWMPDYNRAK RREDPLPKLR GTYVTTKGAM EEPVRDLAEQ G RLPKPPKS FKTDDTLQNS LPFDGITNYT AEYPPKEVPM TRSAARDTKP IALPENRDFQ TASSSAYQVP PVHMRHDCPA TF VPIRPAS RDGHIKLSVY NIPSGDTMY

Macromolecule #29: DMIP2

• Macromolecule: Name: DMIP2 / type: protein_or_peptide / ID: 29 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 24.484893 KDa

Sequence

String:

MSKKYKSFYE NDGSGRDTFI NYAGAYWMNP LPAGTFLKSR EHPSQQRTPT ENGTSLVMDE SIPFPGRTLF GKSRYGTTIL AADQAAALD ATADSKSGHC AGMGSTSSST ANRMAGMDAD GQGFDGEATA LEHISFDVSQ PERRLWQPGH ATTGLEGTGG G STYNGVKD PWTAPNAVDS TLLRLAPAYE STCHYQLRTG RFYTEDAAPP HEMTLPDKGV TWGKSRYY

UniProtKB: Uncharacterized protein

Macromolecule #30: DMIP3

• Macromolecule: Name: DMIP3 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 25.872684 KDa

Sequence

String:

MPPKKKAFMY SIDPELYARS HAPASTIAMG MKSSQASSLC WVNQVRKEEQ IRELWDAAYD PQHIREKKAL QAVQQTRARD AARHEAYVN PESNPELYRI LYMKEPPSSV NNAAAAATAS SSSPQPNTTT EAAPAGVVKG TGDPSNNAFS LSARSVTQEY L QARCRNHT LQQRYPSGPF TAAQAVGWAA GSSDPASASS GEASSSLYVQ VRRRQIGAYR KPEDEDHALL FGYDYTPK

UniProtKB: HMG box domain-containing protein

Macromolecule #31: DMIP4

• Macromolecule: Name: DMIP4 / type: protein_or_peptide / ID: 31 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 30.468156 KDa

Sequence

String:

MSWAPTCQHG SYHQCECYGH DTQLPNYRRD ISKPRTLQGY YAPNGGCPCP PTCSAEKSGL YCSVRGADTS AMPKVTFRAA DGPHTVHVA ADVDGNGRDP ATSIRTAFPN SRNYGGTVAQ RGPTTLSTRA QLVASRKACA EAKREERAQD RKNFLRSVLR D EMLAHMDA EGKRAALEAD MGLMGTHHGE EYEASQRAAG KLRSGLDSTS RDPSTVLTPG EQYREIMDEL EYVAGQPVGS KY NIIRLHY LVEEENRLNQ LRLQKRVQKT AEAMQSKK

UniProtKB: Uncharacterized protein

Macromolecule #32: DMIP6

• Macromolecule: Name: DMIP6 / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 32.116299 KDa

Sequence

String:

MTTLEKQLQA STLSRQCRVD LLSHAQELER SSWGLSKKMI TLPDIKLPPA TTMSTDNSVK RSVRSSVAGA NKERAQEYTA RKQEKEDMI SSRITSDTAL SGTYFKDCSH KFVKGARGFS DPFRTSRSRT ALSGRDMDFV YRYAEASTGS VIERPQPADD A SVAATAAY RKAFQQYKGR LPTTEEMKEA GRLVYAFDEN GCIGVIQKPH SCYPHMHISA VKKPIRACPY QVYSPEVPPV RA ARSEFPG PIPTWSSTKR TTTMATNGTY QPGHHQSTSL KREAANTVVG SR

UniProtKB: Uncharacterized protein

Macromolecule #33: DMIP7

• Macromolecule: Name: DMIP7 / type: protein_or_peptide / ID: 33 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 34.757172 KDa

Sequence

String:

MNSTYKEPSS AAVPTSYAVL SLPSKATMRR KGYNPDEVNY NGGLATHPLA SWKTFSLPVG CTYKDAVTAV QTANAKPWGP IKIRLNFSD GRYEQFERVA PSVMDSLQST TTYSPNGVFK EETLSLSTTR REAQKPRLRP LVDERGHHLS SKPIPRTFAP E ELYKNCPP PVLCQPGYDF TPISYNTFLL NPQDPPHGVR SVQSNFMHSK CDYRPRSYLR PEEVTGTSHA SRHCHCNEVF QL GDHTMDF ACEGTMVDHR NRLVKKDYSP IGTLKANSSI VGRRHARKPR FGCSSMAESQ PVAAAVAPAA DEETV

UniProtKB: Uncharacterized protein

Macromolecule #34: DMIP8

• Macromolecule: Name: DMIP8 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 35.479879 KDa

Sequence

String:

MNTRRKMVQS KPNVPSTHWH LMSEDFYPIF EADKRQHVEY WYNMTATGLQ RDIFRRICNA IYQQDPTTPD PTFNVAFTET VELEQVRLL LKNYGVVLSD NVGKPKTRVW LLHCGKSSRD RNEFRSVFTG CQTTYERRSV MNTDYVAPDP DSYPDDRTHF V RSVDWTSL KTREEMAKLE AQRRANGGVM GSQLEKLPSI IAEERRRMNN GGKPLQRTTK PKNQFDGVNV LSSLGFDGVP AE EALAQLK ANRDAHREAE WYYVEKDGST VYKARITKGA TVGAAASSSQ RGEGGILSSV TSEPMTRWVN KTCS

UniProtKB: Uncharacterized protein

Macromolecule #35: DMIP9

• Macromolecule: Name: DMIP9 / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 35.540793 KDa

Sequence

String:

MTEGRLETIH NLRPKNWDNR RHWTNWHHLY DCEQDHITRE SAPFHDLRAG GQFQYEYAGG GPYAAPIEPQ HLNKRSDGDR MDFALGRGT DMGGMGYLPL DTDPGRPVKP NNVPRQTAMF TRKDPLKRSL FSEYPYMPEG DGDGTGPSPR TGVMKAGGPC D YLGGAAEH IPDPYNDKPN RGRIWHFRTG PVKHDYPKWM PQGEPERKPR KTYGPFRAGK PDHLHIGGDV EWIPDPYDKG PI RNNRHPF RTWHTRTKWS MPVHAPWSTG LATSEARKGA GLDLTENNLP SLKTYRKANL TQTIGAEAAL SSMPSGM

UniProtKB: Uncharacterized protein

Macromolecule #36: DMIP10

• Macromolecule: Name: DMIP10 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 37.695625 KDa

Sequence

String:

MLNDSEHHLV DVAFQSMDTN NTGMVSLSEV KKRFFAHAHP RVKEGSMAPS AARDTLDYHF GLCAADHEGS ITFDEFLKYH EKLADEAYD EHVGDVAAFT EKTIMELWRL GDVLLPTGVR PAFPVTQKPA GLYAVALMTL VWVERRQVAA SPDSGHQSFG S GGAAHNDL FVLRGIKDVV RPIFARGDLP EELQGYFAYP EELAGMAIDY VAPRLSIQRW YDFTWEYSEG KYCGVEGIIS TR VDLESLP AGLRQFVCEH VTAVARGTTW MPTTLTTNPM YKKTSSAYGC GVNEECRKIH HWKVKTFEGK QYGNQYHGLC GKY FSKLGA PLKSSTATGI NM

UniProtKB: EF-hand domain-containing protein

Macromolecule #37: DMIP11

• Macromolecule: Name: DMIP11 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 38.623461 KDa

Sequence

String:

MNHLSTRRPG ATVTPGSALS GTLYVSHNTR PARTMCWMKL AVFTQFPVVP SLAGTHVQSR QDLLSSTAGA GTPEETSLFL QIELFDDEC PQLCANFRRL CNGQSSTRQE QVYCYQGITP SYCGTYFHKI IPSYCAQGGD ITMRVKPGGT NSYSSAGRIW L PDEFKKRR HNEIGLVSMA NNGPDSNGSQ FFITTSAANE RAFNGRHCCI GHVVHGLDAF MALVAPFGNI EGHPSKYVVI VD CGEGKAP TMRLASPSSA AVPQAWMAIS ETATPLSAAF EEAPTEVVVP DCEQPPELQL QHDHAADDGS VMAAVSPSRA VAD MATPST SFGQPLKSSL KGKTTAAWEA AKRHDKHISY QL

UniProtKB: Cyclophilin, putative

Macromolecule #38: DMIP12

• Macromolecule: Name: DMIP12 / type: protein_or_peptide / ID: 38 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 39.016898 KDa

Sequence

String:

MSDVLCKTAV NTRASLASRL PKKREEVPTA SASLHRKIET TYDTAYVSHP ARCFTKVHGA DGATECHLAA DGTALASGEQ TYTLHRHPT SAWESTSHAA TRNVQAQCPL DFYASARKIE REDDDQRGGL VGRMQRSLDI RTKRAEDARL RPPTPTYNPI D PRSIPSAM SEEKFGARSN YLDIVECTQL KPETEEQIGY DSAPLSRLPC GTLSQDLKVQ SKVITQMGTS NELFCGTPKY LA DTSVTYM GYVPIAKHNV PCINHGNDIR RLFAKSTMTM SEHGGGVDIA VIGSSLVTRH RGGKNARAPR ALEPKTPDVI NQT VEGRML QQTLYGTLER ERQINIREDA QGHRYF

UniProtKB: Uncharacterized protein

Macromolecule #39: DMIP13

• Macromolecule: Name: DMIP13 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 39.095578 KDa

Sequence

String:

MGYGGPRVAF YTANQYNNFA VAIDHENAAR VRSILHRRNW PAEQEAQRQR EEAFRSYERA QADEAARERA EEAEALEWRR IKDERTQRS LANKTRRRSL TAAEVTARRA RHARLCRLAE PRKFPEAMYG IGMIGGTVPH QSGRDGKLPA MGRNGAVYCA G VDRDRIVP SRPALDGTVP PWMGSYSTHH GSNQLQAQMA KEAAAYYCTH PAPQRSASEQ EMRQKRLQSI AAEHSVAPQP EG SDLGASE ASDENRGFSV ADEVQQHGMG SDYVDDEVRS IFSHGSSNGP IEATGNMHIS TCSTKPSVQL HLSRYGGAMT APR PARPFK ATLESFTLPE LADRLVQMQI PKPVAR

UniProtKB: Uncharacterized protein

Macromolecule #40: DMIP14

• Macromolecule: Name: DMIP14 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 40.517352 KDa

Sequence

String:

MSCLNNNTIR EDVVFKRRVK NVLLRHGGTG GLVKVLIDFN ARQAVAVRQQ RQATDTNAGS SCTDVGLSYA QFVRFLQDYG VELTPFEAA YLCRAFDDHG SGFISDETFT RHLTGLNERR LRVIKKAWHS LEKRSCADGK VSRELLLSTF EAVAAERARA A AGDLGSRA DSTAHDAAAM TSLSSPVGSA LQATFSAGDE TCEPLKQAYM ASMMSCNSGC MAKGSTAAGC ADDAPGEAAG RT SYAEYLA FYAGVSPQFS TDEAFVTHVL QSWAADDATR PALDETERKW GPDGDPLALD GPRYVKDALH LELGISSKSY NYG HMQREH PYVEPLPPLK RSDIMTSTIQ RTYVPFNNAE QMLADPLVTR RGQLR

UniProtKB: EF-hand domain-containing protein

Macromolecule #41: DMIP15

• Macromolecule: Name: DMIP15 / type: protein_or_peptide / ID: 41 / Number of copies: 11 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 42.884211 KDa

Sequence

String:

MPLPPVESGP GAMTRRNFLV ETNRLRVSQY EPLRKQLEEE DLRIATIRQE EKRARHAEWT ASRRKAADSY NADGTNSGRP LPGSVARAM RASGKTLPEK SAYVLQKEEE AAKKREYNRL YEQDAKEQLA VRAATLKQMR DDEARQMEAL RKLNEEQNCK V AEAHAKAM EEERQYMERL KQSNKRELAA KKAQQQAREA SDRQLQELVN ENNRHRSEMD ERRQKNVTRM LQLQNEEFHR EA MKNKKAS REEHLEEIAA MEERNRRLTK EEQEAAQRKK EQFRQDFEDC IARDKEFRRK HNYDEPAEVT RERNELAARS YRL VLQEER LRDAERRQQY RKDLMDQIMA KETYRMTHLG EAGI

UniProtKB: Flagellar protofilament ribbon protein-like protein

Macromolecule #42: DMIP16

• Macromolecule: Name: DMIP16 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 44.827879 KDa

Sequence

String:

MPIRRALLTA VEVAVAREAC EPVLSSVTHR LLRGGFPEYV KFRQAYAKEC ERAVASDKST SAALPSIGAF PPPPPQTRRV AAVGHRADF SSSFVSVPQI SRRRINPEGL KAVCAESGVL LTSENYAAIF LAYSDPVGFV LADDLLEALH PCRQTPPALL K FVSEVMLS TLFSTLPLSC VALTVDSVRD AFSAIFAASL SREEERAVDA QTAADQLSAL VVAQADVQAT FTPIVYTEGS AV PRDDVTT FIGLILQQHP CLSALIQARC NSVASFLQLL PALASPSQGE ASPLAASMAT STNDSALFSI HHVGSTTKRK FER YEENKD RRDEWIRGRE EAGARPMYMR HTAGYGGHLP EYQYHFGRTF HVIEEDLPQL TKPKPPLEPV PADWHGPGVV LNDS RMNLH HYRLA

UniProtKB: Uncharacterized protein

Macromolecule #43: DMIP17

• Macromolecule: Name: DMIP17 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 38.751098 KDa

Sequence

String:

MLLLPEMVDT PPYPLLPSCS LLPLQGIASL LPCHCSDTHI HTHTHIYIYI YIFTGFDMKH STASPSSSLE LKKKPFLADI LNKAAERLG HKQLWRVASQ LERLPSPASA DVARDALRDI CVFLSDEDYA HMCETYATDT ADGQQSSRMI DTAALIESLS T APLTPRRR HVVNLAVHKL DPNATGIISY ITLSDHYDVL RHPEVRNGAR SEDDVLLAFF DNFCAVDGAP MVQLTTAEMR MY FVGISMR MKDDTDFELW CTRAFCLDRP KLDYSQEMEK LTGSRSHSRL SRLLGEGRQH PLYTTTNEEY GKESTSSDYK LPQ HGRPQQ FTRNLKLRTG GATSMNM

UniProtKB: EF-hand domain-containing protein

Macromolecule #44: DMIP18

• Macromolecule: Name: DMIP18 / type: protein_or_peptide / ID: 44 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 46.871492 KDa

Sequence

String:

MSVRMNASLY SDVVRIERGD YLRFHCEQLS ADGRDTQRYF FGCYYPRWHG FYLEEVRSII GNMGYCELKH FPAYPFDVYL KPADVTAAA DSAKNCDADN ANATTYITDD FQVDNILVLG PPQNQRDDAV KRFKIVSVDT SHLKSKTFSL APVANLNSSS V QNPDTMLS TLRAPGGERV PVQLNSSVLD VLTQLRDAYI DHAGGGIPEI GIKAMGRPFR KVSDDGRRWM TRDGVRQLVR GS RAFGAHA DCLSDTRHAL QTIEDMTDTI FNAFPHEEAT YPVAPGEEAC EERIDYDVFM DYIRGHMNST RKKAVFEVFQ QLD YDSDSN ITIKDIQATF NAQEHPVVVS DAIFTAEKLL KGFLSIWDEN QRYFGLVPYT EFMDYYNGLS AIIEDDAVFL GILK TTWKV PNWTIKFV

UniProtKB: Calcium-binding protein, putative

Macromolecule #45: DMIP19

• Macromolecule: Name: DMIP19 / type: protein_or_peptide / ID: 45 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 48.416961 KDa

Sequence

String:

MQLPQLARNT NGALAYSQRS ADSVPADEDA LKEFLKSRGR EGFAASAYWG ATGEVPPRAP DDVVSKRPYP FQAEINRKLV PLPKDQTSH PRTLDYTTVG FHLILDDGNS NTGGFNASQL QKLREGGDDP DGTQGQGKNG SRKPRFLTLQ TTNQAIPRTF D DGVLAALR DGQAKEDALK GELDNLGATP YANGAAPDTF RLTSDDYCDF SKMDPSAYRQ EDWKDDGDGV FVYKSRYNNV ER EGPRRRE HTFQTLQRGR TADHTKLRST LEDSHKKALP EGYEPYSAKD WMSTTYREHA AYDVAEARHM NDRDATVPLR NTH YALSQA QEMALTQRDA RFQTRHDGKW ATTYSTGYQD RSAEADVCHK YGAKAVFDIQ DGIYTINHEY HHPREEVRTG ETYT PAEMV PGQYTTMYNE PLQAPNNVIG STRRC

UniProtKB: Uncharacterized protein

Macromolecule #46: DMIP20

• Macromolecule: Name: DMIP20 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 49.477602 KDa

Sequence

String:

MTRACARAHT RTEDVLSMNR DPQNALLLEH TAQKTKRLLL QRSPSAVNSI RSFSRSLGIA DDLGGTQVTA EKLRHALQEN NVFLEEHEI QNVFTVLDRG GQGTIDPTDF ISVMYNSISP LRKVWLRRVW RLFIKDPEDG SVQVSELQRQ FMAQGHPSVV R LEATAEEV RRDFQSAFSE STNPDGKISA QEFAQYYAGV SASCNKDECF VAILRGVWPL PGVSRDFSTS LAKGDAQYQG FY HTEQSLP EKTAVSSREA ARSALMRMIR CEHAPTVLSS SAAARALCLS LAQADEARSG FVSEAVFMGA LRAHRLYVPN TSV LECLDT NGDGSVDIKY YEELLLPSPS AARLMLLERL WSRCFENKDT AYRVPVQDLH RKYHASSPED KDGFLTAWDV RTAL GGKVE LEELIQWYVP QSVAVQRDKD FEQLLRRQWG TYDE

UniProtKB: Calcium-binding protein, putative

Macromolecule #47: DMIP21

• Macromolecule: Name: DMIP21 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 50.008688 KDa

Sequence

String:

MIGGEYKKER FSERLTRAQN QPKNRGYLPD THLKTGGYGT GTLMGNWSEE RSDAGYYDGK AVVASTLRPV WSTTYREMVQ NVAAPVDFG ASASGADSSR VGASCTSAPT RCDRTQFSQQ TFMVIEDRTG RSYPGHQPHL DPEWQVSIQS AHYSTSHSSY I HPDVQLQE AKAYVPPVLG GKSSSQSTGV LLRLRRQLEL AQESMGRSIG ASTVVSAFPG NVIRSVRNAL AEACTDSKGN IN TNELQEG FAAAGVTAVP AECVALLRNF DCEGHLTAPY VVIVDALRGE MNCRRADLVE GVYDLLRSFS TDGVVRLDKL VEW VDVEQL PAVKSGDVSA DAARTAFAEQ WDARSATAHI SKERFADFFA DVSFEIPQDN TFELLLRNIW HLSGGRGTCE NTSC RRVEV VHTNGRVTKE EIKNDLLIKD NGDDAAVESL LHANLAKQGI KDVKSVRVVT PV

UniProtKB: Calcyphosin

Macromolecule #48: DMIP22

• Macromolecule: Name: DMIP22 / type: protein_or_peptide / ID: 48 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 52.058332 KDa

Sequence

String:

MIRDQCHAYC RCSYCAQEPQ KSCNTGASMW ATMEALDRPG MSKDELYMKS LRQSLRAKAF LDSSKNAGKF IPDEQLLSSI KRRDAKDLA LTAELVPVRR RDRLQELVLL ENKHRLREEA ALRAERRALG LPEEEEEEEP VSTAHSDSPA STRPSSKESM Q QRLSSGAA TPASRCSAEH DLHAMLKGIK NIVDEDPAPS SHPLTREQVY KLRQLMHTQK KKTREMVDNC ENQPHVCGHC LD VDVQARH LSALPNCSVA YGLTGKVINP EEGIKFGTHT QREPNGMFNT STADAAEVSG RRSRLTSYNA HPYGTGVVFL PVS SLNGKS QNAVGSQSTR FRASFNNNIG KGEPVEEELQ FPGSTYNVYD SGFAKATTPQ TETKANKAER TVKIDTASSN ESSQ HGVNE LKVAAIAAPE QPTYNSSAAM WMTTNQDREL DMKRFLEFQK KLKTNSKRVY EETASRSAKA LPA

UniProtKB: Uncharacterized protein

Macromolecule #49: DMIP23

• Macromolecule: Name: DMIP23 / type: protein_or_peptide / ID: 49 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 64.778984 KDa

Sequence

String:

MAYSNVQQYS PGVLIGNWFE DIALREDQLK LYKNQRHVAM ENPEKAATNA VARSILASQL EEPTTVVAVK GEDVRIGQPY SLVNDKTQS VLALDLMTDG GTSSQSQQYT LSGALVSGPQ VRSTWTLLRC EDEHNHSYNR SSLDVLHYGQ RVRIANENVS P EGFLYVQS SLSSGLHSSQ AQYAVAALNT CADNVFVVSR AGTVRDDVHF GFPVKVGDGV VFLHSLTNLP LACNGERVAT SY GMEYAIT CGYTTDYCSR SRGAVVVKPE NIFYFSGGDG SDPALMKSRM SIKHLSRSST ALKMTAGTGV TVLLERIRQG ALA IGGRIG FRSLSIALGV ACNEQRQRRF LDSNGLRKAI ARLGVLLSPI EVDVLMKRFD TTGNNVVCAQ DFLAELRGTM PLVR MQAVI YAYQQLSIEG RGSVEFKDMR SLFCLNAAII PDVVDGVIQR EEAVLDFESC WPGRVGCKIG TVTLDEFVEY YTDLS PAEE SDERFCELLQ KSWAVPATST YLSGEPHRLL TVTYDDKPTE TVSLPDTLVL DTQDRNAVKR LLIQRGLRGV KDFTVS TTM SSVAREKEDT EDERHGRGGE EKHVS

UniProtKB: EF-hand domain-containing protein

Macromolecule #50: DMIP24

• Macromolecule: Name: DMIP24 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 19.253312 KDa

Sequence

String:

MYVCVCVCAR MAPAFQRGHM TASLLGFTSF SLPLKRKNRF WIGTRYCVAD ALALLSNLTL SSAPRPARLQ LLIPTPIQPA THSPHPQRA QSQTHIRVHP PTHPPTHPPP THKDFPATTM QSTYPKQNCG CDFCCSRRSV NAKKLAASQA AFQRSGIMQR N GCNCCCHS GGPDAVSS

UniProtKB: Uncharacterized protein

Macromolecule #51: DMIP24

• Macromolecule: Name: DMIP24 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 32.014104 KDa

Sequence

String:

FDPEYPVPVN QTALNASRGR NGVPEGDQYT IRNNSTREGG PGASQTADRN GLVKETAEQR ETREAAELDA LERLLMLEYK ARTQATMEA NRLKRNATSA TNPGPKPLPE GYTSGSIDGR MGYSPYGTEA LGGTAAGEGS FGSGACTIHE AYAMAHDCPA E PPAQCHAS HRLQDTMDDV RKVTADPVHT GNRRALQKLL HQHGMAESPA SDNGAAAHKT TSGTELNAFG TSLAQIRTSY AH PRGAGVV WVPTQVSAAA SPSATKDSDA LVVEHSAAAG SGNSKCPPGL SPGAAAALAT GEKQGSYAAC H

UniProtKB: Uncharacterized protein

Macromolecule #52: DMIP25

• Macromolecule: Name: DMIP25 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 43.062922 KDa

Sequence

String:

MATSARHTPT PCLLISSFLP SSPPTPSSLP PNACARVLRK ISTASPLSAP VRAGGKGRGP PHTHTHTHTH TLISQVRAPM LSDRDPSGD GFNNPYENAT LVGNWLEDRL QERIVHEGRG RVGTTWCAKG IFEDPAVTRG RSPAQVQAAL LHSTYEVDYT H PLGMTQHT GGSADMGDCA AASTLMRGSK HKADMVDPAS VSLTAKGGID RALLFSVDPV ADPAAPLPLS TYADTYGAFY YA NDDRSID GNGTRHAGSA ATGTTASSPA GQSNGGATAP PTGADRNLYD SVSRAVVAAA APASSFRTTM AQTQRSADAE AAL SRLPAP RHNMLSRGVA TTSAFGHTGW TLASLSATSA VGHDGATQWQ PLQRHRPPKK ASMSDERWCS SKQAADTPVE HFTL QQRLP PVRM

Macromolecule #53: DMIP26

• Macromolecule: Name: DMIP26 / type: protein_or_peptide / ID: 53 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 42.725043 KDa

Sequence

String:

MSCEPNRPVV DATAAVAAPK LMTIKEAIDR DGVPATFSSK QRRLMDVHIT AYPGIVNQHM LPKHRTAAND LSSIDPLQYR KAHTVRNNN VTSYTEKEEN VVDMITTNQH ANRMTRLGIR LREERAKNRE GMPSSSSLAP RGYHSRFLSP SFDERSTYRW D YCQQEPDG FHDTVRRKLL AAYRDDWTED EKADVAISMK IPYLTMAVSS DRLKDKATVY RKDFMKDATQ AEYRSLTYKT NL EDLNPSY AYSAYSVDDP RRKVFFDSSI AALKTPHSVL EKTFSPRNSF LEKSSSGKQQ SSAREELERL RAMQRELMRL SAA KRSAMA SIYHDEETKT VVIPGIKGSG YRRATNGNNS YVCLPTDHFC YQQVDDF

UniProtKB: Uncharacterized protein

Macromolecule #54: DMIP27

• Macromolecule: Name: DMIP27 / type: protein_or_peptide / ID: 54 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 47.948129 KDa

Sequence

String:

MTSPRHDALG MECVLPPHGP ANMMSTYMTR YDYDYNRDPG LTGSDDGNGN GFEQSISGEV RNRPIFAYGV DTGDRDDRTI YQVDYTPKD RSVKNMAGLA AALTGPAAST AAQGIGDMDN STAYQHHWYR PAVSSHIVLA DGSHPHTVYQ DDYQAPRHVA G GTMKDPVC YAGAAAAAYN ADLLKPHGVI EDDGADLAPK ITSTESLLPR KRASGNAAWT PIFENTADHR HTWNDCVPGD GV VGTYTCT SWDYGDTSKA HPSQLPVSLA RSLTAQNLDA TANPDKAALL RRLAGKHYFS TTLRGPSRGA PLDTNDRTNP QFL SRMEQK QCSVSRRLEK EHYVTMSIYK SDFIDQGLLP ELPGNPGGPA DPHDTHTSPL ATAGEMMAGQ LTNRTRELGL LRNA HGTLK AGEAPAASRV GTGVVSAMRS MEKTASIDKA DPHRHKLR

UniProtKB: Uncharacterized protein

Macromolecule #55: ArcMAP2

• Macromolecule: Name: ArcMAP2 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 20.891818 KDa

Sequence

String:

MACSPEGRIV MAPGSVYLTP EQEERLVERL YTQSLQHKEA TLAELDARYY PVAPLQTISE ETLQKSVQRQ VDVEMERRQQ RRREMDAMA VGEATGPAAG RRSAASKKKF SPEETDTSVR RLYDETLAQK KLKMAESARL YEFHPEDIKS TKMSKAALQE S VNRMSKPK KTEFTIAEVN KIYGL

UniProtKB: Uncharacterized protein

Macromolecule #56: ArcMAP1

• Macromolecule: Name: ArcMAP1 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 30.210736 KDa

Sequence

String:

MLCFLLYPLY MALVSRIVSH VRLNAPLSPR FSKTRKKTPS PISFSTRLKS ICSSVVTVRG ISLALIDIMV ENNDSHVAGK RLTQEELDQ HIRRLTMPRR EAEVHDPFEV CPTKRVPAET LSKMTDRLYT KSLQQKQERL AAADQAAYGD HKRSALLRAE P LSPEDQET SVKRLFNDAL ERKQANMEQL RRQHQYQRRT DETKVPLKTF VQHMYYDRLE AKKKTEKRLY DTYLAPTEIH TG TISRQQA DEASNRLCTT KSGV

UniProtKB: Uncharacterized protein

Macromolecule #57: ArcMAP4

• Macromolecule: Name: ArcMAP4 / type: protein_or_peptide / ID: 57 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 23.592498 KDa

Sequence

String:

MTATENYKGV AENRLSPEEE ENLVQRLYYR QMKLMEQREE ERQAALERAR AQTKKPISKD EEGRLVSRMY DQQVERFANS KAERDRKVE EEKHRNDKKM DSSEIDDQVR RMYEDELQRS QARREELNSR YMPTAAPKKI GKKELKGCVE RLSHVDWEKR D EELFKKYV YPYDPKTTRI SRDDEKAMAD RLSTTKGAG

UniProtKB: Uncharacterized protein

Macromolecule #58: ArcMAP3

• Macromolecule: Name: ArcMAP3 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 23.019268 KDa

Sequence

String:

MSTERDPCLH TKVMTEEELQ RSVDRLSRSN QIKVELKPLA PRRIITREAL EKRIHHLYDE SLQRRQRERE EMALQVDAAI KKGATMPAR TISSEEEEAL VRHLYDETLA IQKRNFDELY RQETSHYERN TKKLTAQKQL AAMDRLYREG MERERKKHIA L YEKYVVAR RQPPPQRTAA ELAESADKMT KGEGLTS

UniProtKB: Uncharacterized protein

Macromolecule #59: CFAP96

• Macromolecule: Name: CFAP96 / type: protein_or_peptide / ID: 59 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 33.732098 KDa

Sequence

String:

MFSPPVYLSV GDEYDKREVH AKTTGQPPFV VSAKHAPHIT DATFSPFVSL STGDPYTSVG FAGPFRMNSA ARDKAEHPNH NAVAPPPFR PVGASKKSSG KGDYYGTFSE KAPIRHETEF PPIKTAADCK AEMAAAKCNF IVNPPKKGTY GYVGLTIGKA G EVQYVADP YEGHIRREAL QKKYEATKRV GSPFRSAVRS GGYFDETKHG VSRVYGLTKP LPPKRSPSSQ QRLSSGPAGR KP WVPGGAL VPDLMKQPEY QEDPYDVKEQ KARAMRQRES AYKPWVPAGN DPYRYIYTKP INYDPPAVV

UniProtKB: Cilia-and flagella-associated protein 96

Macromolecule #60: CFAP97

• Macromolecule: Name: CFAP97 / type: protein_or_peptide / ID: 60 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 18.835117 KDa

Sequence

String:

MQPQRIRNMS SLEREQERNY WKHIERLNNQ KCYIDNRTPE SCAYVRPIGS TRGNPARAQQ ISRDNQKLVE KMVHIMNTRG GVDTSEPWR DHNKAINSQR LRDQRLATIA QENAKLLERL EHAQPTYRAD KFEADRRRNE EFAARASRYP YQPMDRTSC

UniProtKB: Uncharacterized protein

Macromolecule #61: DMAP1

• Macromolecule: Name: DMAP1 / type: protein_or_peptide / ID: 61 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 32.976953 KDa

Sequence

String:

MVFSIPSYLG VGDRYKKPID ARAQGATFLV SRPKTGTNPD ALFDKELRHF WNGERGGDPD AIRRREQLES AKRNLTPNGF VYSSPTPAP AGLGSYYGCF QSTPYIHIPE FPEKHDEAVT KRELPPRGVY TSPAQKGSGY GYAHVALSDV GSNYIATIYD Q QRINERCE REMWKKKMPA EPFRPAGREG FTFDESLATG ASRCYIMTVP FQEKRPEPAF AHFCIDQPWR PAGYVDDKPT TL EYWEDPY RGYDPRKSPK EYVRKPSDAV FLPSSHNDNF WYTQSIVFKR I

UniProtKB: Uncharacterized protein

Macromolecule #62: DMAP2

• Macromolecule: Name: DMAP2 / type: protein_or_peptide / ID: 62 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 52.924051 KDa

Sequence

String:

MICCRSAAAG RGTPKARGCN IGAPVSKPGS SILCTAFSCL TRMNKEAAWL CGRGFSPCSL SLGLLPLCRA QERTHDPCQT KRVGDAHMP ESCTSISFIP PHERVPLLSH LYCLLQFSFP LHPPPPRCQA SSLSSPAVSN MTDKSAYGGL FQDPPYLSSD L DPKKTDPY DKRDAIPSRY LGKSMTIGGR VPPGQHPDAY FEKRFLTLAS SEQNGGKPVG YDEGKGLTAA SKKNTKHPIS DK EFLYSSY PEKSTGPGSY YGCFQNRPHE YMTEPWDDGK SKKKRKAKKP KEEEPTHLPN VKTNPAKKGT YGYPGLLLSN PSY DDNWKK NYAAAEADRA RRERKQGPPP KALGPPLHIP GVSHPYIDEM PNTGVSAVYA AYEPPADSAA AKRKAKREAA AAPT LHQTR AFYGSANRSG EQGCLNPFPN LWVHPNETQP SKIAQRKQAQ KEKEEAARKP KLTEVWKPNS FEKTSVISSC LRRFY

UniProtKB: Uncharacterized protein

Macromolecule #63: DMAP7

• Macromolecule: Name: DMAP7 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 63.279562 KDa

Sequence

String:

MRFAPLSSSL SCSSAFPHLA SCEMLGGFTN YAHLRLRDHR PGNGDYELPP EFGPNSSSSK RSILARHYTP TGGESCSVGP GAYALPTTI GTGRSTRFSP MLSAGNGSTV KVSAGQCASD RPLAAAATEP KQYSMALCPD TPSYSLGSRL KSEWDQQSAA A SPGPAAYD VPGYFDTLDP RQQQQHPCSG PVPGVHLGIR TVLPESRERA GVPGPGAYHL VRFGDELPRT REPLITGSRR GC GTVAGTP GPGAYDDPTT IGYRAGQVQR RRFFTQCSTF GGRWRRREFH TTGPGPAAYN TLASTKRLEK NQRHAPKFVR RQP PLDRLS VAEDAAARTK LSGALTQQAF PTLPSDFDYD CRKGKTIGGK LHKPLARTAP SHLTQAAADK PSGSRAFWES PPPP GGRFA ATPFDSQAQS LAAEKAVADA ARLSEGGSCK PGPGSYTVEV KLTTRRVPAS LFGRMLSAKS VSVENGVPGP GHYRV IDEM GNRGTIFYKG DFHPRGCGGQ SGPAAESVGV GPGAHYNDDT MYSSSINGDR VNNKGFTMGT RYPARATYQH CAPYDE TTN INCVYDDELN WMAQTIQHLP QIQRGKL

UniProtKB: Sperm-tail PG-rich repeat

Macromolecule #64: distal DC1

• Macromolecule: Name: distal DC1 / type: protein_or_peptide / ID: 64 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 75.528688 KDa

Sequence

String:

MNSTATESIA KKRQSVLDVG QRNQLEAIQA EVRRNNEQLT SLRRENKELR LVLQQTSRGQ RNISVEDHYA KEEELLCNKM CVLKRSLNA VKGKNTELLK EIDRANEENR FIQQEGNGVL EEDSAVAHKI RALENRLDKC LVKHNEVQTI RRTYETLLER L QLEQAGFN TQVSSTEQAL QCADKELGEL VAVWKSAAKA RDAAKSEVSD LKAQLVAERH KQRKDLEERR AFIESKRQQL AK THDALLQ KITQQEERHA RAIQSMNSTA GGSVRRRGHR SRASSTSSLS PEDVEQLQKQ KTAYLRLRDV TMGTNVVDVL EKL RERSDH HLQLKTTAAE LETSMEQLEK EKSALQSLWE DVNHRVGHAL VSARVMRAAR DMHSGSDKEN GTLHGTDGRD GEGD SHRNA AGVVGGADTI DYGLLADRRH ERRLVLEEFR QHLRQRQDEL EEAEAKHNGL SQILREADLG VLYLAEKLSI AGAQP VSVN TAADTPATSQ QQKQRTSTQT SLTIDLLRNC SAKLQLMLEE LSPEELEAVV RSLKRAKFAI PDTNVRVPEV LEAQRA HSQ QRAQQITSAS RLRGSPPHTG GASTSQAPMG APGGGPSSKV RRAGSTDSDT SAAVSGAAAA ASATAVLTED YPENEIH DR QELKMMSLAT VEREQQKARK RQMQLRRSKE EMV

UniProtKB: Axonemal dynein intermediate chain protein

Macromolecule #65: distal DC2

• Macromolecule: Name: distal DC2 / type: protein_or_peptide / ID: 65 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 70.576641 KDa

Sequence

String:

MSVVAAKKKG AVSDTLRRSR IEDDIVGAQD QCARQQEQIL HMTEENDQIK KEINALSGTH YDYVKADKLA SLQSEVDGLE RRYQFEKMR KNDLTKRYQL ARIDLLHSRK MKGGVNVEKE HAAAVQRQVD ILESRLDQTL GQFNDALSYN KELRDSIDVI R EERRVFLR VHKRMEDDLK TKKRLMAEHI EKSNRDMDDR DAQLREVERL RAALAEQRQA YAEQLRELDT AMLDIKAMRD EQ TEMQLEL EAREYEFDER MGDSKSARAQ EEADTFRRMI GASVSGTSEE RVSTTGGDDD DDTHSLLYVE KESATITSIL SQI KDALQT DDIDELRAKY LHTGDLNFSL YKYVNELSAK KEALRDSIRD LQRLLNEEED SERQHRALIK GLEEELAETE SKLD EMSSS TALLREAVQR TAATTEDVYA HVGCQQMADS AVAGEERCTE ANMKQFLGTI EERAAHILIA FQRHHQFPVH LRTSP VSSS RAIRDPQSPK GTVQSLEEED NALTANTTDS PHGETLQMAE DWEAEASPIM PIAPQTAHSA VSARNLVRMM ELPSAS LAG GATDRDNNLY ADQDDDHIVS HEDIRKQMEA RLAMMREREE RTQRKKKDTA QKAK

Macromolecule #66: distal DC4

• Macromolecule: Name: distal DC4 / type: protein_or_peptide / ID: 66 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 56.663172 KDa

Sequence

String:

MSRTQQGHTW MKNHPLEGDD IVAGLYNILA KRNPKTMKAC RGIRIPDTVV FEHNFPRGWY TTDMKAKEVV RKQGKDLDAN TIEQGFKQN LYDGSPIAAT YLCTMEKTTD NGETEVNTLV EVFNRDTLAA FLARKVKPDG ILQKFIFPKG YQNSVIRVVW S PRICMVQR RTNKYRIFDR KRAECDPFSI TVTYDGPTFL SDEGSVSGNI AIELKELCGN IVQHFYYTEH KYITRMVLYF KG DKHDRLW LLWCGSLRVS DRKTPSEMPV NLITNFAEPS AAGNTFDEEE LLRKADEAYL RVTNDEMFYE TYMKRMRGGG EAY GEAAAQ GDKRPSSSTE RQQQGHETTE AAADTFDWGG LPPLVLETRD RLFHEKNSMC CLFEDLFYHA RNHFLTRRKE PYYM EIPTE AVQTLTQNGV VDLMRELRLS VKVQDSTASS PMCYVIDAVP HQPLTQLSDK AQEWIQTYFD AKMRVLKQAV QNYTG DESA LCKDVMEL

Macromolecule #67: DC5

• Macromolecule: Name: DC5 / type: protein_or_peptide / ID: 67 / Number of copies: 2 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 11.93878 KDa

Sequence

String:

MPSDAVVMDK IIEGDGKTIP RFGSVVTLDY IGYLQDGSKF DSTLVRGKPF VFRVGCGEVI KGWDAGIVQM SKGERSKLTM PPTLAYGGT GFPGLIPPNE TIVFEVTLLD VS

UniProtKB: peptidylprolyl isomerase

Macromolecule #68: DC6

• Macromolecule: Name: DC6 / type: protein_or_peptide / ID: 68 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Leishmania tarentolae (eukaryote)
• Molecular weight: Theoretical: 19.663977 KDa

Sequence

String:

MACDLTYTDL CAFSNATPKT PPPPVTRTTA VPPLNTTSLI GPPSIVWVLT KTVIALATVV VFSMAQKTQE ALTQEELNLR VRQGLQRRK KCALCTQIFY VNELPGAITH KSILELRQKW GMNVCRGDHM PPPSQLYKRE EICIFCMQFF DTTGSMQTQQ K LLASTRDG ISPSLTLR

UniProtKB: Uncharacterized protein

Macromolecule #69: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 69 / Number of copies: 161 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Macromolecule #70: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 70 / Number of copies: 158 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #71: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 71 / Number of copies: 158 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #72: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 72 / Number of copies: 29 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #73: CALCIUM ION

• Macromolecule: Name: CALCIUM ION / type: ligand / ID: 73 / Number of copies: 3 / Formula: CA
• Molecular weight: Theoretical: 40.078 Da

Macromolecule #74: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 74 / Number of copies: 2 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: filament

Sample preparation

• Concentration: 6.5 mg/mL
• Buffer: pH: 7.2
• Grid: Model: Quantifoil R2/1 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK IV
• Details: This sample consisted of freshly splayed Leishmania tarentolae axonemes.

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 4 / Number real images: 37665 / Average electron dose: 62.6 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.5 µm
• Sample stage: Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

Startup model

Type of model: EMDB MAP
EMDB ID:

20631

Details: The map used for initial angular assignment is the C. reinhardtii DMT.

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 533420
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION

Atomic model buiding 1

• Initial model: Chain - Source name: Other / Chain - Initial model type: integrative model; Details: The initial model consisted of rigid body fit AlphaFold models and models build with ModelAngelo.

Output model

PDB-9e78:
48-nm repeat of the Leishmania tarentolae doublet microtubule