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Structure paper

タイトル	Integrated cryoEM structure of a spumaretrovirus reveals cross-kingdom evolutionary relationships and the molecular basis for assembly and virus entry.
ジャーナル・号・ページ	Cell, Year 2024
掲載日	2024年7月6日
著者	Thomas Calcraft / Nicole Stanke-Scheffler / Andrea Nans / Dirk Lindemann / Ian A Taylor / Peter B Rosenthal /
PubMed 要旨	Foamy viruses (FVs) are an ancient lineage of retroviruses, with an evolutionary history spanning over 450 million years. Vector systems based on Prototype Foamy Virus (PFV) are promising candidates ...Foamy viruses (FVs) are an ancient lineage of retroviruses, with an evolutionary history spanning over 450 million years. Vector systems based on Prototype Foamy Virus (PFV) are promising candidates for gene and oncolytic therapies. Structural studies of PFV contribute to the understanding of the mechanisms of FV replication, cell entry and infection, and retroviral evolution. Here we combine cryoEM and cryoET to determine high-resolution in situ structures of the PFV icosahedral capsid (CA) and envelope glycoprotein (Env), including its type III transmembrane anchor and membrane-proximal external region (MPER), and show how they are organized in an integrated structure of assembled PFV particles. The atomic models reveal an ancient retroviral capsid architecture and an unexpected relationship between Env and other class 1 fusion proteins of the Mononegavirales. Our results represent the de novo structure determination of an assembled retrovirus particle.
リンク	Cell / PubMed:39013471
手法	EM (単粒子) / EM (サブトモグラム平均) / EM (トモグラフィー)
解像度	3.4 - 13.5 Å
構造データ	17311 8ozj EMDB-17311, PDB-8ozj: In situ cryoEM structure of Prototype Foamy Virus Env dimer of trimers 手法: EM (単粒子) / 解像度: 3.7 Å 17312 8ozk EMDB-17312, PDB-8ozk: In situ cryoEM structure of the Prototype Foamy Virus capsid, icosahedral map 手法: EM (単粒子) / 解像度: 3.89 Å 17313 8ozl EMDB-17313, PDB-8ozl: In situ cryoEM structure of the Prototype Foamy Virus capsid, pentamer localised reconstruction 手法: EM (単粒子) / 解像度: 3.4 Å 17314 8ozm EMDB-17314, PDB-8ozm: In situ cryoEM structure of the Prototype Foamy Virus capsid, hexamer 1 localised reconstruction 手法: EM (単粒子) / 解像度: 3.4 Å 17315 8ozn EMDB-17315, PDB-8ozn: In situ cryoEM structure of the Prototype Foamy Virus capsid, hexamer 2 localised reconstruction 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-17316: In situ subtomogram average of Prototype Foamy Virus Env trimer 手法: EM (サブトモグラム平均) / 解像度: 4.9 Å 17317 8ozp EMDB-17317, PDB-8ozp: In situ subtomogram average of Prototype Foamy Virus Env pentamer of trimers 手法: EM (サブトモグラム平均) / 解像度: 11.9 Å 17318 8ozq EMDB-17318, PDB-8ozq: In situ subtomogram average of Prototype Foamy Virus Env hexamer of trimers 手法: EM (サブトモグラム平均) / 解像度: 10.0 Å EMDB-17319: In situ subtomogram average of the Prototype Foamy Virus capsid, wild-type Gag 手法: EM (サブトモグラム平均) / 解像度: 10.2 Å EMDB-17320: In situ subtomogram average of the Prototype Foamy Virus capsid, p68 Gag 手法: EM (サブトモグラム平均) / 解像度: 13.5 Å EMDB-17321: Cryotomogram of Prototype Foamy Virus particles, wild-type Gag 手法: EM (トモグラフィー) EMDB-17322: Cryotomogram of Prototype Foamy Virus particles, p68 Gag 手法: EM (トモグラフィー)
化合物	ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM ChemComp-NAG*: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	eastern chimpanzee simian foamy virus (ウイルス)
キーワード	VIRAL PROTEIN / membrane fusion / envelope glycoprotein / foamy virus / MPER / transmembrane / MEMBRANE PROTEIN / capsid / Gag / pentamer / hexamer