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Yorodumi- PDB-8ozk: In situ cryoEM structure of the Prototype Foamy Virus capsid, ico... -
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-Basic information
Entry | Database: PDB / ID: 8ozk | ||||||
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Title | In situ cryoEM structure of the Prototype Foamy Virus capsid, icosahedral map | ||||||
Components | Gag polyprotein | ||||||
Keywords | VIRAL PROTEIN / capsid / Gag / foamy virus | ||||||
Function / homology | Function and homology information host cytoskeleton / microtubule-dependent intracellular transport of viral material towards nucleus / viral release from host cell / host cell / viral nucleocapsid / host cell cytoplasm / symbiont entry into host cell / host cell nucleus / DNA binding / RNA binding / cytoplasm Similarity search - Function | ||||||
Biological species | Eastern chimpanzee simian foamy virus | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.89 Å | ||||||
Authors | Calcraft, T. / Nans, A. / Rosenthal, P.B. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Cell / Year: 2024 Title: Integrated cryoEM structure of a spumaretrovirus reveals cross-kingdom evolutionary relationships and the molecular basis for assembly and virus entry. Authors: Thomas Calcraft / Nicole Stanke-Scheffler / Andrea Nans / Dirk Lindemann / Ian A Taylor / Peter B Rosenthal / Abstract: Foamy viruses (FVs) are an ancient lineage of retroviruses, with an evolutionary history spanning over 450 million years. Vector systems based on Prototype Foamy Virus (PFV) are promising candidates ...Foamy viruses (FVs) are an ancient lineage of retroviruses, with an evolutionary history spanning over 450 million years. Vector systems based on Prototype Foamy Virus (PFV) are promising candidates for gene and oncolytic therapies. Structural studies of PFV contribute to the understanding of the mechanisms of FV replication, cell entry and infection, and retroviral evolution. Here we combine cryoEM and cryoET to determine high-resolution in situ structures of the PFV icosahedral capsid (CA) and envelope glycoprotein (Env), including its type III transmembrane anchor and membrane-proximal external region (MPER), and show how they are organized in an integrated structure of assembled PFV particles. The atomic models reveal an ancient retroviral capsid architecture and an unexpected relationship between Env and other class 1 fusion proteins of the Mononegavirales. Our results represent the de novo structure determination of an assembled retrovirus particle. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8ozk.cif.gz | 507.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8ozk.ent.gz | 384.2 KB | Display | PDB format |
PDBx/mmJSON format | 8ozk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8ozk_validation.pdf.gz | 1.8 MB | Display | wwPDB validaton report |
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Full document | 8ozk_full_validation.pdf.gz | 1.9 MB | Display | |
Data in XML | 8ozk_validation.xml.gz | 88 KB | Display | |
Data in CIF | 8ozk_validation.cif.gz | 131 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oz/8ozk ftp://data.pdbj.org/pub/pdb/validation_reports/oz/8ozk | HTTPS FTP |
-Related structure data
Related structure data | 17312MC 8ozjC 8ozlC 8ozmC 8oznC 8ozpC 8ozqC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
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Noncrystallographic symmetry (NCS) | NCS oper:
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