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Structure paper

タイトル	High-resolution electron cryomicroscopy of V-ATPase in native synaptic vesicles.
ジャーナル・号・ページ	Science, Vol. 385, Issue 6705, Page 168-174, Year 2024
掲載日	2024年7月12日
著者	Claire E Coupland / Ryan Karimi / Stephanie A Bueler / Yingke Liang / Gautier M Courbon / Justin M Di Trani / Cassandra J Wong / Rayan Saghian / Ji-Young Youn / Lu-Yang Wang / John L Rubinstein /
PubMed 要旨	Intercellular communication in the nervous system occurs through the release of neurotransmitters into the synaptic cleft between neurons. In the presynaptic neuron, the proton pumping vesicular- or ...Intercellular communication in the nervous system occurs through the release of neurotransmitters into the synaptic cleft between neurons. In the presynaptic neuron, the proton pumping vesicular- or vacuolar-type ATPase (V-ATPase) powers neurotransmitter loading into synaptic vesicles (SVs), with the V complex dissociating from the membrane region of the enzyme before exocytosis. We isolated SVs from rat brain using SidK, a V-ATPase-binding bacterial effector protein. Single-particle electron cryomicroscopy allowed high-resolution structure determination of V-ATPase within the native SV membrane. In the structure, regularly spaced cholesterol molecules decorate the enzyme's rotor and the abundant SV protein synaptophysin binds the complex stoichiometrically. ATP hydrolysis during vesicle loading results in a loss of the V region of V-ATPase from the SV membrane, suggesting that loading is sufficient to induce dissociation of the enzyme.
リンク	Science / PubMed:38900912
手法	EM (単粒子)
解像度	3.2 - 3.9 Å
構造データ	44350 9b8o EMDB-44350, PDB-9b8o: Synaptic Vesicle V-ATPase with synaptophysin and SidK, State 3, Vo 手法: EM (単粒子) / 解像度: 3.2 Å 44351 9b8p EMDB-44351, PDB-9b8p: Synaptic Vesicle V-ATPase with synaptophysin and SidK, State 3, V1 手法: EM (単粒子) / 解像度: 3.2 Å 44352 9b8q EMDB-44352, PDB-9b8q: Synaptic Vesicle V-ATPase with synaptophysin and SidK, State 3, peripheral stalks 手法: EM (単粒子) / 解像度: 3.8 Å 44353 9brd EMDB-44353, PDB-9brd: Synaptic Vesicle V-ATPase with synaptophysin and SidK, State 3 手法: EM (単粒子) / 解像度: 3.5 Å 44354 9brc EMDB-44354, PDB-9brc: Synaptic Vesicle V-ATPase with synaptophysin and SidK, State 2 手法: EM (単粒子) / 解像度: 3.9 Å 44355 9brb EMDB-44355, PDB-9brb: Synaptic Vesicle V-ATPase with synaptophysin and SidK, State 1 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-WJP: methyl (3R,6Z,10E,14E)-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraen-1-yl dihydrogen diphosphate ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-LP3: (7R)-4,7-DIHYDROXY-N,N,N-TRIMETHYL-10-OXO-3,5,9-TRIOXA-4-PHOSPHAHEPTACOSAN-1-AMINIUM 4-OXIDE / O-(1-O-ステアロイル-L-グリセロ-3-ホスホ)コリン ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM
由来	rattus norvegicus (ドブネズミ) legionella pneumophila subsp. pneumophila str. philadelphia 1 (バクテリア) legionella pneumophila subsp. pneumophila (バクテリア)
キーワード	PROTON TRANSPORT / Membrane / Synaptic / Complex / Mmebrane / Native / Vesicle