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Structure paper

TitleStructure and Function of Stator Units of the Bacterial Flagellar Motor.
Journal, issue, pagesCell, Vol. 183, Issue 1, Page 244-257.e16, Year 2020
Publish dateOct 1, 2020
AuthorsMònica Santiveri / Aritz Roa-Eguiara / Caroline Kühne / Navish Wadhwa / Haidai Hu / Howard C Berg / Marc Erhardt / Nicholas M I Taylor /
PubMed AbstractMany bacteria use the flagellum for locomotion and chemotaxis. Its bidirectional rotation is driven by a membrane-embedded motor, which uses energy from the transmembrane ion gradient to generate ...Many bacteria use the flagellum for locomotion and chemotaxis. Its bidirectional rotation is driven by a membrane-embedded motor, which uses energy from the transmembrane ion gradient to generate torque at the interface between stator units and rotor. The structural organization of the stator unit (MotAB), its conformational changes upon ion transport, and how these changes power rotation of the flagellum remain unknown. Here, we present ~3 Å-resolution cryoelectron microscopy reconstructions of the stator unit in different functional states. We show that the stator unit consists of a dimer of MotB surrounded by a pentamer of MotA. Combining structural data with mutagenesis and functional studies, we identify key residues involved in torque generation and present a detailed mechanistic model for motor function and switching of rotational direction.
External linksCell / PubMed:32931735
MethodsEM (single particle)
Resolution2.98 - 6.45 Å
Structure data

10828

6ykm

EMDB-10828, PDB-6ykm:
Structure of C. jejuni MotAB
Method: EM (single particle) / Resolution: 3.1 Å

10829

6ykp

EMDB-10829, PDB-6ykp:
Structure of unplugged C. jejuni MotAB
Method: EM (single particle) / Resolution: 2.98 Å

10830

6ykr

EMDB-10830, PDB-6ykr:
Structure of a protonation mimic of unplugged C. jejuni MotAB
Method: EM (single particle) / Resolution: 3 Å

EMDB-10831:
Structure of S. oneidensis MotAB
Method: EM (single particle) / Resolution: 3.48 Å

EMDB-10832:
Structure of V. alginolyticus PomAB
Method: EM (single particle) / Resolution: 6.45 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • Campylobacter jejuni subsp. jejuni 81-176 (Campylobacter)
  • campylobacter jejuni subsp. jejuni serotype o:23/36 (strain 81-176) (Campylobacter)
  • Shewanella oneidensis (bacteria)
  • Vibrio alginolyticus (bacteria)
KeywordsMEMBRANE PROTEIN / Bacterial flagellar motor / stator unit / locomotion / proton transport / ion transport

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