+
検索条件

キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

-
Structure paper

タイトル	Structure and Function of Stator Units of the Bacterial Flagellar Motor.
ジャーナル・号・ページ	Cell, Vol. 183, Issue 1, Page 244-257.e16, Year 2020
掲載日	2020年10月1日
著者	Mònica Santiveri / Aritz Roa-Eguiara / Caroline Kühne / Navish Wadhwa / Haidai Hu / Howard C Berg / Marc Erhardt / Nicholas M I Taylor /
PubMed 要旨	Many bacteria use the flagellum for locomotion and chemotaxis. Its bidirectional rotation is driven by a membrane-embedded motor, which uses energy from the transmembrane ion gradient to generate ...Many bacteria use the flagellum for locomotion and chemotaxis. Its bidirectional rotation is driven by a membrane-embedded motor, which uses energy from the transmembrane ion gradient to generate torque at the interface between stator units and rotor. The structural organization of the stator unit (MotAB), its conformational changes upon ion transport, and how these changes power rotation of the flagellum remain unknown. Here, we present ~3 Å-resolution cryoelectron microscopy reconstructions of the stator unit in different functional states. We show that the stator unit consists of a dimer of MotB surrounded by a pentamer of MotA. Combining structural data with mutagenesis and functional studies, we identify key residues involved in torque generation and present a detailed mechanistic model for motor function and switching of rotational direction.
リンク	Cell / PubMed:32931735
手法	EM (単粒子)
解像度	2.98 - 6.45 Å
構造データ	10828 6ykm EMDB-10828, PDB-6ykm: Structure of C. jejuni MotAB 手法: EM (単粒子) / 解像度: 3.1 Å 10829 6ykp EMDB-10829, PDB-6ykp: Structure of unplugged C. jejuni MotAB 手法: EM (単粒子) / 解像度: 2.98 Å 10830 6ykr EMDB-10830, PDB-6ykr: Structure of a protonation mimic of unplugged C. jejuni MotAB 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-10831: Structure of S. oneidensis MotAB 手法: EM (単粒子) / 解像度: 3.48 Å EMDB-10832: Structure of V. alginolyticus PomAB 手法: EM (単粒子) / 解像度: 6.45 Å
化合物	ChemComp-HOH: WATER / 水
由来	Campylobacter jejuni subsp. jejuni 81-176 (カンピロバクター) campylobacter jejuni subsp. jejuni serotype o:23/36 (strain 81-176) (カンピロバクター) Shewanella oneidensis (バクテリア) Vibrio alginolyticus (バクテリア)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Bacterial flagellar motor / stator unit / locomotion / proton transport (プロトンポンプ) / ion transport