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- EMDB-46669: Mycobacteriophage Bxb1 portal and connector assembly - Composite ... -

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Basic information

Entry
Database: EMDB / ID: EMD-46669
TitleMycobacteriophage Bxb1 portal and connector assembly - Composite map and model
Map dataMycobacteriophage Bxb1 portal and connector assembly - Composite map and model
Sample
  • Virus: Mycobacterium phage Bxb1 (virus)
    • Protein or peptide: Portal protein
    • Protein or peptide: Head-to-tail adaptor
    • Protein or peptide: Head-to-tail stopper
    • Protein or peptide: Tail terminator
    • Protein or peptide: Major tail protein
KeywordsBacteriophage / portal / VIRAL PROTEIN
Function / homologyMycobacteriophage D29, Gp19 / Phage protein Gp19/Gp15/Gp42 / Portal protein / Phage portal protein, SPP1 Gp6-like / Tail terminator / Major tail protein / Head-to-tail stopper / Head-to-tail adaptor / Portal protein
Function and homology information
Biological speciesMycobacterium phage Bxb1 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsFreeman KG / White SJ / Huet A / Conway JF
Funding support United States, Taiwan, 8 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)K99AI173544 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM116884 United States
Howard Hughes Medical Institute (HHMI)GT12053 United States
National Institutes of Health/Office of the DirectorS10 OD025009 United States
National Institutes of Health/Office of the DirectorS10 OD019995 United States
National Institutes of Health/Office of the DirectorS10OD032467 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)U24GM129547 United States
Academia Sinica (Taiwan)AS-IDR-110-06 Taiwan
CitationJournal: To Be Published
Title: Structure and infection dynamics of mycobacteriophage Bxb1
Authors: Freeman KG / Mondal S / Macale LS / Podgorski J / White SJ / Silva B / Ortiz V / Huet A / Narsico JT / Ho MC / Jacobs-Sera D / Lowary TL / Conway JF / Park D / Hatfull GF
History
DepositionAug 20, 2024-
Header (metadata) releaseSep 25, 2024-
Map releaseSep 25, 2024-
UpdateSep 25, 2024-
Current statusSep 25, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_46669.png

Downloads & links

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Map

FileDownload / File: emd_46669.map.gz / Format: CCP4 / Size: 427.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMycobacteriophage Bxb1 portal and connector assembly - Composite map and model
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.12 Å/pix.
x 482 pix.
= 539.84 Å		1.12 Å/pix.
x 482 pix.
= 539.84 Å		1.12 Å/pix.
x 482 pix.
= 539.84 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.12 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 6.0
Minimum - Maximum-20.836480999999999 - 50.460650000000001
Average (Standard dev.)0.0013719201 (±0.9962859)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions482482482
Spacing482482482
CellA=B=C: 539.84 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Mycobacterium phage Bxb1

EntireName: Mycobacterium phage Bxb1 (virus)
Components
  • Virus: Mycobacterium phage Bxb1 (virus)
    • Protein or peptide: Portal protein
    • Protein or peptide: Head-to-tail adaptor
    • Protein or peptide: Head-to-tail stopper
    • Protein or peptide: Tail terminator
    • Protein or peptide: Major tail protein

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Supramolecule #1: Mycobacterium phage Bxb1

SupramoleculeName: Mycobacterium phage Bxb1 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all
Details: Portal and connector complex of Bxb1, containing five protein subunit types. This is a composite map, and related entries for consensus and locally refined maps are noted.
NCBI-ID: 2902907 / Sci species name: Mycobacterium phage Bxb1 / Sci species strain: Mycobacterium phage Bxb1 / Virus type: VIRION / Virus isolate: SPECIES / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)

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Macromolecule #1: Portal protein

MacromoleculeName: Portal protein / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium phage Bxb1 (virus)
Molecular weightTheoretical: 53.822113 KDa
SequenceString: MAETESIDPE KLRDQLLDAF ENKQNELKSS KAYYDAERRP DAIGLAVPLD MRKYLAHVGY PRTYVDAIAE RQELEGFRIP SANGEEPES GGENDPASEL WDWWQANNLD IEATLGHTDA LIYGTAYITI SMPDPEVDFD VDPEVPLIRV EPPTALYAEV D PRTRKVLY ...String:
MAETESIDPE KLRDQLLDAF ENKQNELKSS KAYYDAERRP DAIGLAVPLD MRKYLAHVGY PRTYVDAIAE RQELEGFRIP SANGEEPES GGENDPASEL WDWWQANNLD IEATLGHTDA LIYGTAYITI SMPDPEVDFD VDPEVPLIRV EPPTALYAEV D PRTRKVLY AIRAIYGADG NEIVSATLYL PDTTMTWLRA EGEWEAPTST PHGLEMVPVI PISNRTRLSD LYGTSEISPE LR SVTDAAA QILMNMQGTA NLMAIPQRLI FGAKPEELGI NAETGQRMFD AYMARILAFE GGEGAHAEQF SAAELRNFVD ALD ALDRKA ASYSGLPPQY LSSSSDNPAS AEAIKAAESR LVKKVERKNK IFGGAWEQAM RLAYKMVKGG DIPTEYYRME TVWR DPSTP TYAAKADAAA KLFANGAGLI PRERGWVDMG YTIVEREQMR QWLEQDQKQG LGLIGSLYGA STPEGKPGEA PVGEP PAPE PDAA

UniProtKB: Portal protein

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Macromolecule #2: Head-to-tail adaptor

MacromoleculeName: Head-to-tail adaptor / type: protein_or_peptide / ID: 2 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium phage Bxb1 (virus)
Molecular weightTheoretical: 14.064146 KDa
SequenceString:
MATLATHEDV TAFWARTPTA EEIVLINRRL AQAERMLLRA IPELLIKASS DPVFRAEVID IEAEAVLRLV RNHEGYLSET DGNYTYMLQ AQDPNRKLEI LPEEWEVLGI VRSGLGILVP TVVLPS

UniProtKB: Head-to-tail adaptor

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Macromolecule #3: Head-to-tail stopper

MacromoleculeName: Head-to-tail stopper / type: protein_or_peptide / ID: 3 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium phage Bxb1 (virus)
Molecular weightTheoretical: 14.302012 KDa
SequenceString:
MSLLDRGGTY GSPEDGFDPV TVYPEVTRKD RLGNTLVGPS LTGIETVARF QVQGQSGTSA RRAEMDDIGD MTEQVYTMRL PRSFTTELK SGSEVVWRGE RWGVYGEPRR YKGSRRIAHL EYTVRRF

UniProtKB: Head-to-tail stopper

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Macromolecule #4: Tail terminator

MacromoleculeName: Tail terminator / type: protein_or_peptide / ID: 4 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium phage Bxb1 (virus)
Molecular weightTheoretical: 16.230531 KDa
SequenceString:
MAGKLPIVGE VVLPILRGHE DLSNPISTVP SLAGVHVGTW VEDIDSRTFP LITVRRVGGT RSPEHPTLFT QPVVEMTAYS AADLPTTEQ MYEDALEVLY RAARLQTKTP AGYLHSVTET LGASHGPSPF DRTWRVFGLI RLGIRPPKN

UniProtKB: Tail terminator

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Macromolecule #5: Major tail protein

MacromoleculeName: Major tail protein / type: protein_or_peptide / ID: 5 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium phage Bxb1 (virus)
Molecular weightTheoretical: 30.170271 KDa
SequenceString: MALKDDAVLI AARGYVYTAA VGTAAPTPSQ LKLIDLEHPE AWDRTGWDLV GHTSEDDLPE FGFDGGDSEV RGSWQKKKLR EVETEEIAD YVVINLTQFD ETALELYFGP NQSATPGIFG VKSGSVVNER ALLIVIVDND VRLGFHARKA SLKREDAISL A TDEFGALP ...String:
MALKDDAVLI AARGYVYTAA VGTAAPTPSQ LKLIDLEHPE AWDRTGWDLV GHTSEDDLPE FGFDGGDSEV RGSWQKKKLR EVETEEIAD YVVINLTQFD ETALELYFGP NQSATPGIFG VKSGSVVNER ALLIVIVDND VRLGFHARKA SLKREDAISL A TDEFGALP VRATFLDYQS YNLYEWIEED WFNAVDAPVV YLLDLGGATG GDYTLLVGGK STGDIAYNAN ASAIKTAIGA VD DGVAESA WTVTADGSDF EISGPLAVAL GVDSTTGGSG VTVDVV

UniProtKB: Major tail protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration10 mg/mL
BufferpH: 7.5
Component:
ConcentrationFormulaName
10.0 mMC4H11NO3Tris
1.0 mMMgSO4Magnesium sulfate
68.44 mMNaClSodium chloride
1.0 mMCaCl2Calcium chloride
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 283.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 30.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionApplied symmetry - Point group: C3 (3 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: OTHER / Number images used: 22590
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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