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- EMDB-4370: Unique features of mammalian mitochondrial translation initiation... -

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Basic information

Entry
Database: EMDB / ID: EMD-4370
TitleUnique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit.
Map data
Sample
  • Complex: mammalian mitochondrial translation initiation complex
    • Complex: mammalian mitochondrial ribosome
      • Protein or peptide: x 52 types
      • RNA: x 2 types
    • Complex: Translation initiation factor IF-2, mitochondrial
      • Protein or peptide: x 1 types
    • Complex: tRNATransfer RNA
      • RNA: x 1 types
  • Ligand: x 8 types
Function / homology
Function and homology information


Mitochondrial translation elongation / Mitochondrial translation termination / mitochondrial translational initiation / translation factor activity, RNA binding / ribosome disassembly / mitochondrial translational termination / mitochondrial transcription / mitochondrial translational elongation / translation release factor activity, codon nonspecific / microprocessor complex ...Mitochondrial translation elongation / Mitochondrial translation termination / mitochondrial translational initiation / translation factor activity, RNA binding / ribosome disassembly / mitochondrial translational termination / mitochondrial transcription / mitochondrial translational elongation / translation release factor activity, codon nonspecific / microprocessor complex / Mitochondrial translation initiation / ribonuclease III activity / mitochondrial large ribosomal subunit / peptidyl-tRNA hydrolase / regulation of translational initiation / aminoacyl-tRNA hydrolase activity / mitochondrial ribosome / mitochondrial translation / ribosomal small subunit binding / organelle membrane / RNA processing / rescue of stalled ribosome / translation initiation factor activity / double-stranded RNA binding / large ribosomal subunit / cell junction / rRNA binding / nuclear body / ribosome / structural constituent of ribosome / cell cycle / ribonucleoprotein complex / translation / protein domain specific binding / nucleotide binding / intracellular membrane-bounded organelle / GTPase activity / synapse / GTP binding / positive regulation of DNA-templated transcription / mitochondrion / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / Ribosomal protein L37, mitochondrial / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L48 ...Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / Ribosomal protein L37, mitochondrial / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L48 / 39S ribosomal protein L40, mitochondrial / Mitochondrial ribosomal protein L55 superfamily / Mitochondrial ribosomal protein L37 / Mitochondrial ribosomal protein L55 / Mitochondrial ribosomal protein L46 NUDIX / 39S ribosomal protein L42, mitochondrial / Mitochondrial 28S ribosomal protein S32 / Ribosomal protein L28/L40, mitochondrial / Mitochondrial ribosomal protein L28 / Ribosomal protein L37/S30 / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L51, mitochondrial / Mitochondrial 28S ribosomal protein S30 (PDCD9) / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 / Mitochondrial ribosomal subunit / Mitochondrial ribosome protein 63 / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / Ribosomal protein L35, mitochondrial / 39S ribosomal protein L52, mitochondrial / Mitoribosomal protein mL52 / MRPL44, double-stranded RNA binding domain / Tim44-like domain / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Tim44-like domain / Ribosomal protein L7/L12 dimerisation domain / Tim44 / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / Ribosomal protein L49/IMG2 / Mitochondrial large subunit ribosomal protein (Img2) / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / Ribosomal subunit 39S / 39S ribosomal protein L46, mitochondrial / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein, eukaryotic / Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like / PEBP-like superfamily / Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily / TGS domain profile. / TGS / TGS-like / Peptide chain release factor class I / RF-1 domain / Ribonuclease III, endonuclease domain superfamily / Double stranded RNA-binding domain (dsRBD) profile. / Double-stranded RNA-binding domain / : / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Beta-grasp domain superfamily / NTF2-like domain superfamily / NUDIX hydrolase-like domain superfamily / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L28/L24 superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24
Similarity search - Domain/homology
Large ribosomal subunit protein mL49 / Large ribosomal subunit protein mL62 / Mitochondrial ribosomal protein L18 / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein bL28m / 39S ribosomal protein L20, mitochondrial isoform 1 / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein mL64 / 39S ribosomal protein L23, mitochondrial / Large ribosomal subunit protein bL34m ...Large ribosomal subunit protein mL49 / Large ribosomal subunit protein mL62 / Mitochondrial ribosomal protein L18 / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein bL28m / 39S ribosomal protein L20, mitochondrial isoform 1 / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein mL64 / 39S ribosomal protein L23, mitochondrial / Large ribosomal subunit protein bL34m / Ribosomal protein / Mitochondrial ribosomal protein L12 / Mitochondrial ribosomal protein L41 / Large ribosomal subunit protein uL4m / Large ribosomal subunit protein mL51 / Large ribosomal subunit protein mL37 / Mitochondrial ribosomal protein L57 / Large ribosomal subunit protein mL42 / 39S ribosomal protein L48, mitochondrial / Large ribosomal subunit protein uL24m / Large ribosomal subunit protein uL16m / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein uL22m / Mitochondrial ribosomal protein S18A / Mitochondrial ribosomal protein L2 / 39S ribosomal protein L15, mitochondrial / 39S ribosomal protein L27, mitochondrial / Large ribosomal subunit protein uL11m / Large ribosomal subunit protein mL38 / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein bL21m / Mitochondrial ribosomal protein L4 / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein mL52 / Large ribosomal subunit protein uL29m / Mitochondrial ribosomal protein L39 / Large ribosomal subunit protein mL44 / Large ribosomal subunit protein mL46 / Large ribosomal subunit protein uL30m / 39S ribosomal protein L48, mitochondrial / Large ribosomal subunit protein bL35m / Large ribosomal subunit protein mL50 / Mitochondrial ribosomal protein L55 / Large ribosomal subunit protein mL45 / Large ribosomal subunit protein mL54 / Large ribosomal subunit protein bL9m / Translation initiation factor IF-2, mitochondrial
Similarity search - Component
Biological speciesSus scrofa (pig) / Homo sapiens (human) / Pig (pig)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsKummer E / Leibundgut M / Boehringer D / Ban N
Funding support Switzerland, 3 items
OrganizationGrant numberCountry
European Molecular Biology OrganizationALTF 1196-2014 Switzerland
Swiss National Science Foundation138262 Switzerland
Swiss National Science Foundation310030B_163478 Switzerland
CitationJournal: Nature / Year: 2018
Title: Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM.
Authors: Eva Kummer / Marc Leibundgut / Oliver Rackham / Richard G Lee / Daniel Boehringer / Aleksandra Filipovska / Nenad Ban /
Abstract: Mitochondria maintain their own specialized protein synthesis machinery, which in mammals is used exclusively for the synthesis of the membrane proteins responsible for oxidative phosphorylation. The ...Mitochondria maintain their own specialized protein synthesis machinery, which in mammals is used exclusively for the synthesis of the membrane proteins responsible for oxidative phosphorylation. The initiation of protein synthesis in mitochondria differs substantially from bacterial or cytosolic translation systems. Mitochondrial translation initiation lacks initiation factor 1, which is essential in all other translation systems from bacteria to mammals. Furthermore, only one type of methionyl transfer RNA (tRNA) is used for both initiation and elongation, necessitating that the initiation factor specifically recognizes the formylated version of tRNA (fMet-tRNA). Lastly, most mitochondrial mRNAs do not possess 5' leader sequences to promote mRNA binding to the ribosome. There is currently little mechanistic insight into mammalian mitochondrial translation initiation, and it is not clear how mRNA engagement, initiator-tRNA recruitment and start-codon selection occur. Here we determine the cryo-EM structure of the complete translation initiation complex from mammalian mitochondria at 3.2 Å. We describe the function of an additional domain insertion that is present in the mammalian mitochondrial initiation factor 2 (mtIF2). By closing the decoding centre, this insertion stabilizes the binding of leaderless mRNAs and induces conformational changes in the rRNA nucleotides involved in decoding. We identify unique features of mtIF2 that are required for specific recognition of fMet-tRNA and regulation of its GTPase activity. Finally, we observe that the ribosomal tunnel in the initiating ribosome is blocked by insertion of the N-terminal portion of mitochondrial protein mL45, which becomes exposed as the ribosome switches to elongation mode and may have an additional role in targeting of mitochondrial ribosomes to the protein-conducting pore in the inner mitochondrial membrane.
History
DepositionApr 13, 2018-
Header (metadata) releaseMay 23, 2018-
Map releaseAug 8, 2018-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.12
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.12
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6gb2
  • Surface level: 0.12
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4370.png

Downloads & links

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Map

FileDownload / File: emd_4370.map.gz / Format: CCP4 / Size: 84.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.39 Å
Density
Contour LevelBy AUTHOR: 0.12 / Movie #1: 0.12
Minimum - Maximum-0.3282438 - 0.65797997
Average (Standard dev.)0.0017977863 (±0.0235527)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions281281281
Spacing281281281
CellA=B=C: 390.59 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.391.391.39
M x/y/z281281281
origin x/y/z0.0000.0000.000
length x/y/z390.590390.590390.590
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS281281281
D min/max/mean-0.3280.6580.002

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Supplemental data

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Mask #1

Fileemd_4370_msk_1.map
Projections & Slices
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Half map: #1

Fileemd_4370_half_map_1.map
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Half map: #2

Fileemd_4370_half_map_2.map
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Sample components

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Entire : mammalian mitochondrial translation initiation complex

EntireName: mammalian mitochondrial translation initiation complex
Components
  • Complex: mammalian mitochondrial translation initiation complex
    • Complex: mammalian mitochondrial ribosome
      • Protein or peptide: Mitochondrial ribosomal protein L12
      • Protein or peptide: Mitochondrial ribosomal protein L27
      • Protein or peptide: Mitochondrial ribosomal protein L28
      • Protein or peptide: Mitochondrial ribosomal protein L47
      • Protein or peptide: 'Mitochondrial ribosomal protein L30
      • Protein or peptide: 'Mitochondrial ribosomal protein L55
      • Protein or peptide: Mitochondrial ribosomal protein L32
      • Protein or peptide: Mitochondrial ribosomal protein L33
      • Protein or peptide: Mitochondrial ribosomal protein L34
      • Protein or peptide: Mitochondrial ribosomal protein L35
      • Protein or peptide: Ribosomal protein
      • RNA: 16S ribosomal RNA, mitochondrial
      • RNA: CP tRNAPhe, mitochondrial
      • Protein or peptide: Mitochondrial ribosomal protein L2
      • Protein or peptide: ICT1
      • Protein or peptide: Mitochondrial ribosomal protein L4
      • Protein or peptide: Mitochondrial ribosomal protein L9
      • Protein or peptide: Mitochondrial ribosomal protein L10
      • Protein or peptide: Mitochondrial ribosomal protein L11
      • Protein or peptide: Mitochondrial ribosomal protein L13
      • Protein or peptide: Mitochondrial ribosomal protein L14
      • Protein or peptide: Mitochondrial ribosomal protein L15
      • Protein or peptide: Mitochondrial ribosomal protein L16
      • Protein or peptide: Mitochondrial ribosomal protein L17
      • Protein or peptide: Mitochondrial ribosomal protein L18
      • Protein or peptide: Mitochondrial ribosomal protein L19
      • Protein or peptide: Mitochondrial ribosomal protein L20
      • Protein or peptide: Mitochondrial ribosomal protein L21
      • Protein or peptide: Mitochondrial ribosomal protein L22
      • Protein or peptide: Mitochondrial ribosomal protein L23
      • Protein or peptide: Mitochondrial ribosomal protein L24
      • Protein or peptide: Mitochondrial ribosomal protein L37
      • Protein or peptide: Mitochondrial ribosomal protein L38
      • Protein or peptide: Mitochondrial ribosomal protein L39
      • Protein or peptide: Mitochondrial ribosomal protein L40
      • Protein or peptide: Mitochondrial ribosomal protein L41
      • Protein or peptide: Mitochondrial ribosomal protein L42
      • Protein or peptide: Mitochondrial ribosomal protein L43
      • Protein or peptide: Mitochondrial ribosomal protein L44
      • Protein or peptide: Mitochondrial ribosomal protein L45
      • Protein or peptide: Mitochondrial ribosomal protein L46
      • Protein or peptide: Mitochondrial ribosomal protein L48
      • Protein or peptide: Mrpl34
      • Protein or peptide: Mitochondrial ribosomal protein L50
      • Protein or peptide: Mitochondrial ribosomal protein L51
      • Protein or peptide: Mitochondrial ribosomal protein L52
      • Protein or peptide: mL53, MRPL53
      • Protein or peptide: Uncharacterized protein
      • Protein or peptide: Mitochondrial ribosomal protein L57
      • Protein or peptide: Mitochondrial ribosomal protein L58
      • Protein or peptide: 'Mitochondrial ribosomal protein L59
      • Protein or peptide: mL65, MRPS30
      • Protein or peptide: Mitochondrial ribosomal protein S18A
      • Protein or peptide: unassigned secondary structure elements
    • Complex: Translation initiation factor IF-2, mitochondrial
      • Protein or peptide: Translation initiation factor IF-2, mitochondrial
    • Complex: tRNATransfer RNA
      • RNA: P-site fMet-tRNAMet, mitochondrial
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: GUANOSINE-5'-MONOPHOSPHATE
  • Ligand: SPERMINE
  • Ligand: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE
  • Ligand: SODIUM IONSodium
  • Ligand: N-FORMYLMETHIONINE
  • Ligand: water

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Supramolecule #1: mammalian mitochondrial translation initiation complex

SupramoleculeName: mammalian mitochondrial translation initiation complex
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#56
Molecular weightTheoretical: 2.85 MDa

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Supramolecule #2: mammalian mitochondrial ribosome

SupramoleculeName: mammalian mitochondrial ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#13, #15-#55
Source (natural)Organism: Sus scrofa (pig)

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Supramolecule #3: Translation initiation factor IF-2, mitochondrial

SupramoleculeName: Translation initiation factor IF-2, mitochondrial / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #14
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #4: tRNA

SupramoleculeName: tRNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #56
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: synthetic construct (others)

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Macromolecule #1: Mitochondrial ribosomal protein L12

MacromoleculeName: Mitochondrial ribosomal protein L12 / type: protein_or_peptide / ID: 1
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 21.268975 KDa
SequenceString: MLPAAASSLW GPCFGLRAAA LRVARHQGPR LCGVRLMRCS SHRKGEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKT LKIQDVGFMP MGAVAPGAPP AAAAPEAAEE DLPKRKEQTH FTVRLTEAKP VDKVKLIKEI KSHIQGINLV Q AKKLVESL ...String:
MLPAAASSLW GPCFGLRAAA LRVARHQGPR LCGVRLMRCS SHRKGEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKT LKIQDVGFMP MGAVAPGAPP AAAAPEAAEE DLPKRKEQTH FTVRLTEAKP VDKVKLIKEI KSHIQGINLV Q AKKLVESL PQEIKANVPK AEAEKIKAAL EAVGGTVVLE

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Macromolecule #2: Mitochondrial ribosomal protein L27

MacromoleculeName: Mitochondrial ribosomal protein L27 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 15.943688 KDa
SequenceString:
MALAVLALRT RAAVTALLSP PQAAALAVRY ASKKTGGSSK NLGGKSPGKR FGIKKMEGHY VHAGNILATQ RHFRWHPGAH VGLGKNKCL YALEEGVVRY TKEVYVPNPS NSEAVDLVTR LPQGAVLYKT FVHVVPAKPE GTFKLVAML

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Macromolecule #3: Mitochondrial ribosomal protein L28

MacromoleculeName: Mitochondrial ribosomal protein L28 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 30.148953 KDa
SequenceString: MPLHKVPVGL WKQLRLWEGI YSRLPRHYLR SLEEARTPTP VHYRPHGAKF KINPKNWQRE RVEDVPIPVH YPPESQLGLW GGEGWVLGH RYVNNDKLSK RVRKVWKPQL FQRELYSEIL DKRFTVTVTM RTLDLIDQAC GFDFYILKTP KEDLCSKFGM D LKRGMLLR ...String:
MPLHKVPVGL WKQLRLWEGI YSRLPRHYLR SLEEARTPTP VHYRPHGAKF KINPKNWQRE RVEDVPIPVH YPPESQLGLW GGEGWVLGH RYVNNDKLSK RVRKVWKPQL FQRELYSEIL DKRFTVTVTM RTLDLIDQAC GFDFYILKTP KEDLCSKFGM D LKRGMLLR LARQDPQLHP DDPARRAAIY DRYKAFVIPE AEAEWVGLTL DEAVEKQRLL EEKDPIPLFK IFVEELLGQL QQ QALSEPA VVQTRASRK

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Macromolecule #4: Mitochondrial ribosomal protein L47

MacromoleculeName: Mitochondrial ribosomal protein L47 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 30.085088 KDa
SequenceString: MAAAGLAVFC RRVSAALKAC RLLIRPQAPP STSCRFSPSL LPKNTPNVTS FHQFRIFHTT FSRRGLEEFF DDPKNWGEEK VKSGASWTC QQLRNKSNED LHKLWYVLLK ERNMLLTLEQ EAKRQRLPMP SPERLEKVVD SMDALDKVVQ EREDALRLLQ T GQEKARPG ...String:
MAAAGLAVFC RRVSAALKAC RLLIRPQAPP STSCRFSPSL LPKNTPNVTS FHQFRIFHTT FSRRGLEEFF DDPKNWGEEK VKSGASWTC QQLRNKSNED LHKLWYVLLK ERNMLLTLEQ EAKRQRLPMP SPERLEKVVD SMDALDKVVQ EREDALRLLQ T GQEKARPG AWRRDIFGRI IWHKFKQWPI PWYLNKKYNR KRFFAMPYVE RFVRMRIEKQ ARIKARKRSL ERKKEKFLQE KF PHLSETQ KSSHV

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Macromolecule #5: 'Mitochondrial ribosomal protein L30

MacromoleculeName: 'Mitochondrial ribosomal protein L30 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 18.58584 KDa
SequenceString:
MAGILRSVVQ RPPGRLQTVT KGMESLICTD WIRHKFTRSR IPDKVFQPSP EDHEKYGGDP QYPHKLHIVT RIKSTKRRPY WEKDIIKML GLEKAHTPQV HKNIPSVNAK LKVVKHLIRI KPLKLPQGLP TEEDMANTCL KSNGELVVRW LLNPANQEAR K S

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Macromolecule #6: 'Mitochondrial ribosomal protein L55

MacromoleculeName: 'Mitochondrial ribosomal protein L55 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 15.156537 KDa
SequenceString:
MAAKGSLLRL LWQRVVTGAA PESCRHLYTS SWRADCNRAL LTRLHRQTYA RLYPVLLVKQ DGSTIHIRYR EPRRMLTMPV DLDSLSPEE RRARFRKREA KFKEKKEEPE LSDDFDVEQY KRFWTKK

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Macromolecule #7: Mitochondrial ribosomal protein L32

MacromoleculeName: Mitochondrial ribosomal protein L32 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 21.53793 KDa
SequenceString:
MASAMLVLVV PPWPAARGLL RNWWEQLQRK LQHNRLGLPL HPWGPALAVQ GPAICTEPAN DTNGSKAISS LLDSVFWMAA PKNRRSIEV NRCRRRNPQK LIKVKNNIDV CPECGHLKQK HILCGYCYEK VRKETAEIRR QMGKQEGGPF RAPTTETVVL Y SGETPSEQ DQGKRIIERE RKRPSWFTQN

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Macromolecule #8: Mitochondrial ribosomal protein L33

MacromoleculeName: Mitochondrial ribosomal protein L33 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 7.508097 KDa
SequenceString:
MFLSAVTFAK SKSKTILVKM MSQAGTGFSF NTKRSRLREK LTLLHYDPVV KKKVLFVEQK KIRSL

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Macromolecule #9: Mitochondrial ribosomal protein L34

MacromoleculeName: Mitochondrial ribosomal protein L34 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 10.871729 KDa
SequenceString:
MAFLARSVGR LLDPVSRSAA LVGGRWLQPQ AWLGFPDTWG LPAMQQTRGK ARGNEYQPSN IKRKHKHGWV RRLRTPTGVQ VILRRMHKG RKSLSH

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Macromolecule #10: Mitochondrial ribosomal protein L35

MacromoleculeName: Mitochondrial ribosomal protein L35 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 21.548543 KDa
SequenceString:
MAASAFAGAV RAATGILRPL HILASSAYQN CAKNACLSSV LSSRHFSHIQ TSALLSAPRL ITSVRNLMCG PTAPVLNRVA PLLPHVLKP PVRTVTYFSS RKGKRKTVKA VIYRFLRLHS GLWLRRKAGY KKKLWKKTAA RKRRLREFVF CNKTQSKLLD K MTTSFWKR RNWYADDPYQ KYQDRTNLKV

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Macromolecule #11: Ribosomal protein

MacromoleculeName: Ribosomal protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 10.841016 KDa
SequenceString:
MATAFLRTVL SAVGPLLHLG GRPLSTFAAG PPRAALAVGA QPSPAAALLS ARPLLGPQPA LGFKTKGVLK KRCRDCYLVK RRGRWFIYC KTNPKHKQRQ M

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Macromolecule #14: Translation initiation factor IF-2, mitochondrial

MacromoleculeName: Translation initiation factor IF-2, mitochondrial / type: protein_or_peptide / ID: 14
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 72.811219 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: SGGSGSGWKS QLSSTKSKKV VEVWIGMTIE ELARAMEKNT DYVYEALLNT DIDIDSLEAD SHLDEVWIKE VITKAGMKLK WSKLKQDKV RKNKDAVRRP QADPALLTPR SPVVTIMGHV DHGKTTLLDK FRKTQVAAVE TGGITQHIGA FLVSLPSGEK I TFLDTPGH ...String:
SGGSGSGWKS QLSSTKSKKV VEVWIGMTIE ELARAMEKNT DYVYEALLNT DIDIDSLEAD SHLDEVWIKE VITKAGMKLK WSKLKQDKV RKNKDAVRRP QADPALLTPR SPVVTIMGHV DHGKTTLLDK FRKTQVAAVE TGGITQHIGA FLVSLPSGEK I TFLDTPGH AAFSAMRARG AQVTDIVVLV VAADDGVMKQ TVESIQHAKD AQVPIILAVN KCDKAEADPE KVKKELLAYD VV CEDYGGD VQAVPVSALT GDNLMALAEA TVALAEMLEL KADPNGPVEG TVIESFTDKG RGLVTTAIIQ RGTLRKGSVL VAG KCWAKV RLMFDENGKT IDEAYPSMPV GITGWRDLPS AGEEILEVES EPRAREVVDW RKYEQEQEKG QEDLKIIEEK RKEH KEAHQ KAREKYGHLL WKKRSILRFL ERKEQIPLKP KEKRERDSNV LSVIIKGDVD GSVEAILNII DTYDASHECE LELVH FGVG DVSANDVNLA ETFDGVIYGF NVNAGNVIQQ SAAKKGVKIK LHKIIYRLVE DLQEELSSRL PCAVEEHPVG EASILA TFS VTEGKKKVPV AGCRVQKGQL EKQKKFKLTR NGHVIWKGSL TSLKHHKDDI SIVKTGMDCG LSLDEDNMEF QVGDRIV CY EEKQIQAKTS WDPGF

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Macromolecule #15: Mitochondrial ribosomal protein L2

MacromoleculeName: Mitochondrial ribosomal protein L2 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 33.270934 KDa
SequenceString: MALRVLTRAL SSLSLTPRIA VAPGLNLLPA VQVTNNVLLT LPSGLMSLPC RPILTSVALS ATSVSWKSRT KYTVMPVKMR KSGGRNHTG QIQVHGIGGG HKQRYRMIDF LRFRPEHESK PGPFEEKVIA VRYDPCRSAD IALVAGGNRK RWIIATENMK A GDTVLNSD ...String:
MALRVLTRAL SSLSLTPRIA VAPGLNLLPA VQVTNNVLLT LPSGLMSLPC RPILTSVALS ATSVSWKSRT KYTVMPVKMR KSGGRNHTG QIQVHGIGGG HKQRYRMIDF LRFRPEHESK PGPFEEKVIA VRYDPCRSAD IALVAGGNRK RWIIATENMK A GDTVLNSD HIGRMAVAAR EGDAHPLGAL PVGTLINNVE SEPGRGAQYI RAAGTCGVLL RKVNGTAIIQ LPSKRQMQVL ET CIATVGR VSNVDHNKRV IGKAGRNRWL GKRPNSGLWQ RKGGWAGRKI RPLPPMKSYV KLPSAAAQS

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Macromolecule #16: ICT1

MacromoleculeName: ICT1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 38.427199 KDa
SequenceString: MPGWGLLGRA GARVLGCGAD GLGASRGLGN RTDICLLVRS LHGKSVTWWD EHLSEENVPF VKQLVSDENK AQLASKLCPL KDEPWPIHP WEPGSSRVGL IALKLGMMPL WTKDGQKHVV TLLQVQDCHV LKYTPKENHN GRMAALTVGG KTVSHFHKSA S ILEFYQEL ...String:
MPGWGLLGRA GARVLGCGAD GLGASRGLGN RTDICLLVRS LHGKSVTWWD EHLSEENVPF VKQLVSDENK AQLASKLCPL KDEPWPIHP WEPGSSRVGL IALKLGMMPL WTKDGQKHVV TLLQVQDCHV LKYTPKENHN GRMAALTVGG KTVSHFHKSA S ILEFYQEL GLPPKQKVKI FNVTENAVIK PGTPLYAAHF RPGQYVDVTA KTIGKGFQGV MRRWGFKGQP ATHGQTKTHR RP GAISTGD VARVWPGTKM PGQLGNIDRT AFGLKVWRIN TKHNIIYVNG SVPGHKNCLV KIKDSKLPAY KDFCKNLPFP TYF PDGDEE ALPEDLYDEN VCQPGAPSIT FT

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Macromolecule #17: Mitochondrial ribosomal protein L4

MacromoleculeName: Mitochondrial ribosomal protein L4 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 33.251469 KDa
SequenceString: MLQLVRAGAR TWFRPSGCRG LNTLAEEAVQ QAEKPESVAS LGLQPPVLRK CELPVPAHRR PVQAWIESLR GYEQERVGLT ELHPDVFST APRLDILHQV AIWQKNFKRI SYAKTKTRAE VRGGGRKPWV QKGSGRARHG SIRSPIWRGG GVAHGPRGPT S YYYMLPMK ...String:
MLQLVRAGAR TWFRPSGCRG LNTLAEEAVQ QAEKPESVAS LGLQPPVLRK CELPVPAHRR PVQAWIESLR GYEQERVGLT ELHPDVFST APRLDILHQV AIWQKNFKRI SYAKTKTRAE VRGGGRKPWV QKGSGRARHG SIRSPIWRGG GVAHGPRGPT S YYYMLPMK VRVQGLKVAL TVKLAQDDLH IVDSLELPTA DPQYLIELAR YRRWGDSVLL VDLEHEDMPQ NVVAATSGLK TF NLVPAVG LNVHSMLKHQ TLVLTLPTVA FLEEKLLWHN SRYTPLYPFR LPYCDFP

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Macromolecule #18: Mitochondrial ribosomal protein L9

MacromoleculeName: Mitochondrial ribosomal protein L9 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 30.373449 KDa
SequenceString: MAAAACTAAG GGLLRAGLER LLRGGVRALL RPRLEGVTPR SERAFSLSLS RSTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKDNLELVLT QSVEELGVRG DLVSVKKSVG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKLQTKA G EATVKFLR ...String:
MAAAACTAAG GGLLRAGLER LLRGGVRALL RPRLEGVTPR SERAFSLSLS RSTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKDNLELVLT QSVEELGVRG DLVSVKKSVG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKLQTKA G EATVKFLR RCHLEVGMKN NVKWELNPEI VARHFLRNLG VVVAPHALKL PEEPITQRGE YWCEVTVNGL DTVRVPMSVV NF ERPKTKR YKYWLAQQAA KGDVPTSSQM I

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Macromolecule #19: Mitochondrial ribosomal protein L10

MacromoleculeName: Mitochondrial ribosomal protein L10 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 29.449689 KDa
SequenceString: MAAVVAGMLR GGLLPQAGRL PTFQTVRYGS KAVTRHRRVM HFERQKLMAV TEYIPPKPTI NPRCLPPPPT PPQEETGLVR LLRREIAAV FRDNRMIAVC QHVALSAEDK LLLRHQLRKH KILMKIFPNQ VLKPFLEESK YQNLLPLFVG HNLLLVSEEP K VKEMVRIL ...String:
MAAVVAGMLR GGLLPQAGRL PTFQTVRYGS KAVTRHRRVM HFERQKLMAV TEYIPPKPTI NPRCLPPPPT PPQEETGLVR LLRREIAAV FRDNRMIAVC QHVALSAEDK LLLRHQLRKH KILMKIFPNQ VLKPFLEESK YQNLLPLFVG HNLLLVSEEP K VKEMVRIL KGIPFLPLLG GCIDDTILSR QGFINYSKLP SLALVQGELV GGLTFLTAQT YSMLQHQPRQ LTALLDQYVK QQ QEGHPAV PASGQPEPPD PVPDP

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Macromolecule #20: Mitochondrial ribosomal protein L11

MacromoleculeName: Mitochondrial ribosomal protein L11 / type: protein_or_peptide / ID: 20
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 20.809271 KDa
SequenceString: MSKLSRATRA LKKPEASGMI RAIVRAGQAR PGPPLGPILG QRGVSINQFC KEFNEKTKDI KEGIPLPTKI FVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARHTGKEVA GLVTLKHVYE IARVKAQDDA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ ...String:
MSKLSRATRA LKKPEASGMI RAIVRAGQAR PGPPLGPILG QRGVSINQFC KEFNEKTKDI KEGIPLPTKI FVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARHTGKEVA GLVTLKHVYE IARVKAQDDA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ KERALFLAAQ READLAAQAE AAKK

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Macromolecule #21: Mitochondrial ribosomal protein L13

MacromoleculeName: Mitochondrial ribosomal protein L13 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 20.633801 KDa
SequenceString:
MSSFSKAPQQ WATFARVWYL LDGKMQPPGK LAAMASVKLQ GLHKPVYHQL SDCGDHVVIM NTRHIAFSGN KWEQKVYSSH TGYPGGFRQ VTAAQLHQKD PVAIVKLAIY GMLPKNLHRR TMMQRLHLFP DEDIPEDILK NLVEELPQPR KVPRRLDEYT Q EEIEAFPR VWSPPEDYRL

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Macromolecule #22: Mitochondrial ribosomal protein L14

MacromoleculeName: Mitochondrial ribosomal protein L14 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 15.955632 KDa
SequenceString:
MAFCTGLRGP FAQVSRALSQ RCFSTTGSLS AIQKMTRVRV VDNSALGTTP YHRPPRCIHV YNKTGVGKVG DRILLAIRGQ KKKALIVGH RMPGPRMTPR FDSNNVVLLE DNGNPVGTRI KTPIPSSLRQ REGEFSKVLA IAQNFV

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Macromolecule #23: Mitochondrial ribosomal protein L15

MacromoleculeName: Mitochondrial ribosomal protein L15 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 33.481723 KDa
SequenceString: MGGPVQGGGP RALDLLRALP RVSLANLKPN PGSRKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYLRIPK YGFNEGHSF RHQYQPLSLN RLQYLIDLGR VDPTQPIDLT QLVNGRGVTI QPSKRDYGVQ LVEEGADTFK AKVNIEVQMA S ELAIAAIE ...String:
MGGPVQGGGP RALDLLRALP RVSLANLKPN PGSRKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYLRIPK YGFNEGHSF RHQYQPLSLN RLQYLIDLGR VDPTQPIDLT QLVNGRGVTI QPSKRDYGVQ LVEEGADTFK AKVNIEVQMA S ELAIAAIE KNGGVVTTAF YDPRSLEILC KPVPFFLRGQ PIPKRMLPPE ALVPYYTDAK NRGYLADPAR FPEARLELAR KY GYVLPDI TKDELFKMLS TRKDPRQIFF GLAPGWVVNM ADKKILKPTD ENLLKYYSS

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Macromolecule #24: Mitochondrial ribosomal protein L16

MacromoleculeName: Mitochondrial ribosomal protein L16 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 28.680531 KDa
SequenceString: MWRLLARACA PLLRAPLSDS WAAPSATAGF KTLLPVPTFE DVAIPEKPKL RFVERVPLVP KVRRERKNLS DIRGPSTEAT EFTEGRFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF ALWRVPAPFK PITRKGMGQR MGGGKGAIDH YVTPVKAGRL I VEMGGRCE ...String:
MWRLLARACA PLLRAPLSDS WAAPSATAGF KTLLPVPTFE DVAIPEKPKL RFVERVPLVP KVRRERKNLS DIRGPSTEAT EFTEGRFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF ALWRVPAPFK PITRKGMGQR MGGGKGAIDH YVTPVKAGRL I VEMGGRCE FKEVQGFLDL VAHKLPFPAK AVSRETLEKM RKDQEERERN NQNPWTFERI ATANMLGIRK VLSPYDLTHK GR YWGKFYT PERV

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Macromolecule #25: Mitochondrial ribosomal protein L17

MacromoleculeName: Mitochondrial ribosomal protein L17 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 19.268178 KDa
SequenceString:
MRLSVAAAIS HGRVFRRLGL GPESRIHLLQ NLLTGLVRHE RIEASWARVD ELRGYAEKLI DYGKLGDTNE RAMRMADFWL TEKDLIPKL FQVLAPRFQG QNGGYTRMLQ IPNRNEQDRA KMAVIEYKGN CLPPLPLPRR DSNLTLLNQL LQGLRQDQEA S SHTAQTPA V

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Macromolecule #26: Mitochondrial ribosomal protein L18

MacromoleculeName: Mitochondrial ribosomal protein L18 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 20.582568 KDa
SequenceString:
MALRSRFWAL LSICRNPGCR AAALSTSCKP ATNPETDPVE NEAVAPEFTN RNPRNLELLA VARKERGWGT VWPSREFWHR LRVIRTQHH IEALVEHRNG QVVVSASTRE WAIKKHLYST RNVVACESVG RVLAERCLEA GINFMVYHPT PWEAASDSIK R LQHAMTEG GVVLREPRRI YE

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Macromolecule #27: Mitochondrial ribosomal protein L19

MacromoleculeName: Mitochondrial ribosomal protein L19 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 33.441539 KDa
SequenceString: MAASITRGSW AAVGLGQSFP PTRALLPAPA SVICRTLAGS GRQQVTGSSE PGAFQPPPKP VIVDKRRPQR PETRFLSPEF IPPRGRTNP LKFQIERKDM LERRKILHIP EFYVGSILRV TTADPYASAK TSQFLGICIQ RSGSGLGATF ILRNTIEGQG V EICFELYN ...String:
MAASITRGSW AAVGLGQSFP PTRALLPAPA SVICRTLAGS GRQQVTGSSE PGAFQPPPKP VIVDKRRPQR PETRFLSPEF IPPRGRTNP LKFQIERKDM LERRKILHIP EFYVGSILRV TTADPYASAK TSQFLGICIQ RSGSGLGATF ILRNTIEGQG V EICFELYN PRIQEIQVVK LEKRLDNSLL YLRDALPEYS TFDVNMKPIT QEFNQEVPVN QLKVKMKPKP WSKRWERPKF NI KGIRFDL CLTEEQMREA QKWSQPWLEF DMMREYDTSK IEAAIWDEIE ASKNS

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Macromolecule #28: Mitochondrial ribosomal protein L20

MacromoleculeName: Mitochondrial ribosomal protein L20 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 17.554793 KDa
SequenceString:
MVFLTVPLWL RSRVTDRYWR VQEVLKHARH FRGRKNRCYR LAVRAVTRAF VKCTRARRLK KRSLRTLWIN RITAASQEHG LKYPAFIIN LIKCQVELNR KVLADLAIYE PKTFKSLAAL AKRRREEGFA AALGDGKEPD GIFSRVVQHR

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Macromolecule #29: Mitochondrial ribosomal protein L21

MacromoleculeName: Mitochondrial ribosomal protein L21 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 23.154861 KDa
SequenceString: MAAAVAASVL QGTFGRLVSA YSRSVLRTSR PGTAPPWSAP RRLSSQHTSS LRGCVPKTSL GSPPWPDIVL PDPVEETRHH AEVVGQVNE LIAAGRYGRL FAVVHFAGHQ WKVTAEDLIL IENELDVACG ERIRLEKVLL VGADNFTLLG KPLLGKELVR V EATVIEKT ...String:
MAAAVAASVL QGTFGRLVSA YSRSVLRTSR PGTAPPWSAP RRLSSQHTSS LRGCVPKTSL GSPPWPDIVL PDPVEETRHH AEVVGQVNE LIAAGRYGRL FAVVHFAGHQ WKVTAEDLIL IENELDVACG ERIRLEKVLL VGADNFTLLG KPLLGKELVR V EATVIEKT ESWPKINMKF QKRKNYQRKR ITVNPQTVLR INSIEIAPCL C

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Macromolecule #30: Mitochondrial ribosomal protein L22

MacromoleculeName: Mitochondrial ribosomal protein L22 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 24.313357 KDa
SequenceString: MAAAVLERLG GLWMQNLRGK LALRLLSSHS ILPQSHIHTS ASVEISRKWE KKNKIVYPPQ LPGEPRRPAE IYHCRRQIKY SKDKMWYLA KLIRGMSIDQ ALAQLEFSDK KGAQIIKEVL LEAQDMAVRD HNVEFRSNLY VAESTSGRGQ YLKRIRYHGR G RFGIMEKV ...String:
MAAAVLERLG GLWMQNLRGK LALRLLSSHS ILPQSHIHTS ASVEISRKWE KKNKIVYPPQ LPGEPRRPAE IYHCRRQIKY SKDKMWYLA KLIRGMSIDQ ALAQLEFSDK KGAQIIKEVL LEAQDMAVRD HNVEFRSNLY VAESTSGRGQ YLKRIRYHGR G RFGIMEKV FCHYFVKLVE GPPPPREAPK TAVAHAREYI QELRNRTITH AL

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Macromolecule #31: Mitochondrial ribosomal protein L23

MacromoleculeName: Mitochondrial ribosomal protein L23 / type: protein_or_peptide / ID: 31
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 17.643205 KDa
SequenceString:
MARNVLYPLY QLGNPQLRVF RTNFFIQLVR PGTAQPEDTV QFRIPMEMTR VDLRNYLERI YNVPVAAVRT RVQYGSNRRR DHRNIRIKK PDYKVAYVQL ALGQTFTFPD LFPERKGASV DVDVRDQVLE DQRQKHSPDP RRGGVPGWFG L

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Macromolecule #32: Mitochondrial ribosomal protein L24

MacromoleculeName: Mitochondrial ribosomal protein L24 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 24.893529 KDa
SequenceString: MRLSALLALA SKVTLPPNYR YGMSRPGSLS DKRKNPPGTR RRRVPVEPIS DEDWHLFCGD KVEILEGKDA GKQGKVVQVI RQRNWVVVE GLNTHYRYVG KTVDYRGTMI PSEAPLLHNQ VKLVDPTDRK PTDVEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE ...String:
MRLSALLALA SKVTLPPNYR YGMSRPGSLS DKRKNPPGTR RRRVPVEPIS DEDWHLFCGD KVEILEGKDA GKQGKVVQVI RQRNWVVVE GLNTHYRYVG KTVDYRGTMI PSEAPLLHNQ VKLVDPTDRK PTDVEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE TWIDGPKDTS VEDALEKTYV PRLKTLEEEV MEAMGIQETR RHKKVYWY

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Macromolecule #33: Mitochondrial ribosomal protein L37

MacromoleculeName: Mitochondrial ribosomal protein L37 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 47.917988 KDa
SequenceString: MALASGPARR ALARLGRLGL GGCGAPRRGA YEWGVRSTRK PEPPPLDRVY EIPGLEPITY AGKMHFMPGL ARPVFPPWDP GWTHPKFRR LPPLHEHPLY KDQACYVFHQ RCRLLEGVKQ ALWLTKTQLI EGLPEKVLRL ADDPRNHIEN QDERVLNAIS H ARLWHSTE ...String:
MALASGPARR ALARLGRLGL GGCGAPRRGA YEWGVRSTRK PEPPPLDRVY EIPGLEPITY AGKMHFMPGL ARPVFPPWDP GWTHPKFRR LPPLHEHPLY KDQACYVFHQ RCRLLEGVKQ ALWLTKTQLI EGLPEKVLRL ADDPRNHIEN QDERVLNAIS H ARLWHSTE DIPKRETYCP VIVDSLIQLC KSQILKHPSL ARRICAQNNT LSATWNRESI LLQVHGSSGA RLNAKDPLPP VA SQEEVEA TKNHVLETFY PISPTMGLQE CNVYDVNDDT GFQEGYPYPC PHTLYFLESA NLRPRRFQPD QLRAKMILFA FGS ALAQAR LLYGNDSKVL EQPVVVQSVG TDGRLFQFLV LQLNTTDLAS DEGVKNLAWV DSDQLLYQHF WCLPVIKKKV VVEP VGPIG FQPETFRKFL ALYLHGAV

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Macromolecule #34: Mitochondrial ribosomal protein L38

MacromoleculeName: Mitochondrial ribosomal protein L38 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 44.656387 KDa
SequenceString: MAAPWWRAAL CASRRWRGFS TSAALSRRAA PLGPMPNEDI DVSDLERLKK YRSFDRYRRR AEQEARKPHW WRTYREHFGE ESGPKDRVD IGLPPPKVSR TQQLLERKQA LRELRANVEE ERAARLQTAR IPLEAVRAEW ERTCGPYHKQ RLAEYCGLYR D LFHGATFV ...String:
MAAPWWRAAL CASRRWRGFS TSAALSRRAA PLGPMPNEDI DVSDLERLKK YRSFDRYRRR AEQEARKPHW WRTYREHFGE ESGPKDRVD IGLPPPKVSR TQQLLERKQA LRELRANVEE ERAARLQTAR IPLEAVRAEW ERTCGPYHKQ RLAEYCGLYR D LFHGATFV PRVPLHVAYA VGEDDLMPVY HGNEVTPTEA AQAPEVTYEA DEGSLWTLLL TNLDGHLLEP DAEYVHWLVT NI PGNRVTE GQETCPYLPP FPARGSGFHR FAFLLFKQDK RIDFSGDTRP SPCYQLAQRT FHTFDFYKKH QDAMTPAGLA FFQ CRWDDS VTRVFHQLLD MREPVFEFVR PPPYHPKQKR FPHRQPLRYL DRYRDSHEPT YGIY

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Macromolecule #35: Mitochondrial ribosomal protein L39

MacromoleculeName: Mitochondrial ribosomal protein L39 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 38.523422 KDa
SequenceString: MAMGAWGLRL WRAVPRGEAG WRLIATSPAS QLSPTELIEM QNDLFNKEKN RQLSLTPRTE KIEVKHVGKT DPGTVFVMNK NISTPYSCA MHLSEWYCRK SILALVDGQP WDMYKPLTKS CEIKFLTFKD DDPGEVNKAY WRSCAMMMGC VIERAFKDEY V VSLVRAPE ...String:
MAMGAWGLRL WRAVPRGEAG WRLIATSPAS QLSPTELIEM QNDLFNKEKN RQLSLTPRTE KIEVKHVGKT DPGTVFVMNK NISTPYSCA MHLSEWYCRK SILALVDGQP WDMYKPLTKS CEIKFLTFKD DDPGEVNKAY WRSCAMMMGC VIERAFKDEY V VSLVRAPE VPVIAGAFCY DVVLDKRLDE WMPTKENLHS FTKDARALIY KDLPFETLEV EAKVALEIFQ HNKYKLDFIE EK ASQNPER IVKLHRFGDF IDVSEGPLIP RTSICFQYEV SAVHNLQTQS SLVRRFQGLS LPVHLRAHFT IWNKLLERSR KMV TEDKTK PTEESAST

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Macromolecule #36: Mitochondrial ribosomal protein L40

MacromoleculeName: Mitochondrial ribosomal protein L40 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 24.075658 KDa
SequenceString: MAAAALGAAS RTLRPASRLV GAWPTQTRDA HERGSLFSFW GLVPMRAEPL RKKKKVDPKK DQAAKDRLKK RIRRLEKASQ ELIPIEDFI TPVKFLNKER QRPPVELPFE ESERRALLLK RWSLYKQREH EMERSAIRSL LEAQEEALQE LRLSSPELHA E ATKRDPSL ...String:
MAAAALGAAS RTLRPASRLV GAWPTQTRDA HERGSLFSFW GLVPMRAEPL RKKKKVDPKK DQAAKDRLKK RIRRLEKASQ ELIPIEDFI TPVKFLNKER QRPPVELPFE ESERRALLLK RWSLYKQREH EMERSAIRSL LEAQEEALQE LRLSSPELHA E ATKRDPSL FPFERQGPDY TPPISDYQPP EGRYQDITKV YTQVEFKK

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Macromolecule #37: Mitochondrial ribosomal protein L41

MacromoleculeName: Mitochondrial ribosomal protein L41 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 15.073404 KDa
SequenceString:
MGLLSGAARA LVRGADRMSK WTSKRGPRTF CKGRGAKGTG FHGRDGKFVQ IKEMIPELVV PELAGFKLKP YVNYRAPEGT DTPLTAKQL FLETAAPAIE KDFKAGTFDP EHLEKYGFEP TQEGKLFQLY PKNFPR

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Macromolecule #38: Mitochondrial ribosomal protein L42

MacromoleculeName: Mitochondrial ribosomal protein L42 / type: protein_or_peptide / ID: 38
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 16.680178 KDa
SequenceString:
MALAAVKWVI SSRTILKHLF PIQNSASYCV CHKSTYSSLP DDYNCKVELA LTSDGRTIVC YHPSVDIPYE HTKPIPRPDP VHNIEETHD LVLKTRLEEK GEHLEQGPMI EQLSKMFFTT KHRWYPRGQY HRRRRKPNPP KDR

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Macromolecule #39: Mitochondrial ribosomal protein L43

MacromoleculeName: Mitochondrial ribosomal protein L43 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 17.728109 KDa
SequenceString:
MTARGTASRF LTSVLHNGLG RYVQQLQRLS FSLSRDAPSS RGAREFVERE VTDFARRNPG VVIYVNPRPC CVPRVVAEYL NGAVREESI HCKSVEEIAA LVQKLADQSG LDVIRIRKPF HTDSPSIQGQ WHPFTNKPTT LGGLRPREVQ DPAPAQVQAQ

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Macromolecule #40: Mitochondrial ribosomal protein L44

MacromoleculeName: Mitochondrial ribosomal protein L44 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 37.527195 KDa
SequenceString: MASGLVRLLQ WGPRRLLAPA APTLAPPVRG AKKGFRAAYR FQKELERWRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ETFSLDLLK TAFVNSCYIK SEEAKRQKLG IDKEAALLNL KDNQELSEQG ISFSQTCLTQ FFEDAFPDLP TEGVTSLVDF L TSEEVVCH ...String:
MASGLVRLLQ WGPRRLLAPA APTLAPPVRG AKKGFRAAYR FQKELERWRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ETFSLDLLK TAFVNSCYIK SEEAKRQKLG IDKEAALLNL KDNQELSEQG ISFSQTCLTQ FFEDAFPDLP TEGVTSLVDF L TSEEVVCH VARNLAVEQL ALSAEFPVPP PVLRQTFFAV IGALLQSSGP ERTALFIRDF LITQMTGKEL FEMWTITNPM GL LVEELKK RKISAPESRL TRQSGSTTAL PVYFVGLYCD RKLIAEGPGE TVLVAEEEAA RVALRKLFGF TENRRPWDYS KPK EHVRAE KTITAS

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Macromolecule #41: Mitochondrial ribosomal protein L45

MacromoleculeName: Mitochondrial ribosomal protein L45 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 35.331805 KDa
SequenceString: MAAPVTRGLS CLPRVLGWWS RQPVLVTQST AVVPVRTKKR FTPPTYQPKY KSEKEFVEHA RKAGLVIPHE RLERPIHLAC TAGIFDAYV PPEGDARISS LSKEGLAQRA ERLKKNVASQ LSIRKIRESD PNFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTENC ...String:
MAAPVTRGLS CLPRVLGWWS RQPVLVTQST AVVPVRTKKR FTPPTYQPKY KSEKEFVEHA RKAGLVIPHE RLERPIHLAC TAGIFDAYV PPEGDARISS LSKEGLAQRA ERLKKNVASQ LSIRKIRESD PNFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTENC FPDMVWDIRY KTVRWSFVES LEPPQVVQVR CSSLMNQGNI YGQVTVRMHT RQTLAIYDRF GRLMYGQEDV PR DVLEYVV FEKHLVDPYG SWRMHGKIIP PWAPPKQPIL KTVMIPGPQL KPWEEFEEPQ GEVHKPQPA

+
Macromolecule #42: Mitochondrial ribosomal protein L46

MacromoleculeName: Mitochondrial ribosomal protein L46 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 32.215998 KDa
SequenceString: MAAPVRRTML RVVRGWRRFE GPWAHSLGSR NLALAVAPSS SSSPWRLLGA LCLQRPPLVT KPLTPLQEEM ADLLQQIEIE RSLYSDHEL RALDEAQQLA KKKSDLYEEE DEQNILLAQD LEDMWEQKFL HFKLGARLTE ADKKDDRTSL HRKLDRNLIL L VREKLGDQ ...String:
MAAPVRRTML RVVRGWRRFE GPWAHSLGSR NLALAVAPSS SSSPWRLLGA LCLQRPPLVT KPLTPLQEEM ADLLQQIEIE RSLYSDHEL RALDEAQQLA KKKSDLYEEE DEQNILLAQD LEDMWEQKFL HFKLGARLTE ADKKDDRTSL HRKLDRNLIL L VREKLGDQ DIWMLPQSDW QPGETLRQTA ERTLATLSEN NMEAKFLGNA PCGHYKFKFP QAMRTETSLG AKVFFFKALL LT GDFSQAG KKGHHVWVSK EELGDYLKPK YLAQVRRFLL DL

+
Macromolecule #43: Mitochondrial ribosomal protein L48

MacromoleculeName: Mitochondrial ribosomal protein L48 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 23.749512 KDa
SequenceString: MNGALGKALC LRNDTVLKQA LSLIRVRASG ESPICSAGGI LLSTSRHYRS KPTHGIGRYK HLVKAQEPKK KKGKVEVRPI NLGTDYEYG VLNIHLIAYD MALAESYAQY VHNLCNHLAI KVEESYAMPT KTMEVLQLQE QGSKMFLDAV LTTHERVVQI S GLSATFAE ...String:
MNGALGKALC LRNDTVLKQA LSLIRVRASG ESPICSAGGI LLSTSRHYRS KPTHGIGRYK HLVKAQEPKK KKGKVEVRPI NLGTDYEYG VLNIHLIAYD MALAESYAQY VHNLCNHLAI KVEESYAMPT KTMEVLQLQE QGSKMFLDAV LTTHERVVQI S GLSATFAE IFLEIIHSNL PEGVKLSVRE HTEEDFKGRF KARPELEELL AKLN

+
Macromolecule #44: Mrpl34

MacromoleculeName: Mrpl34 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 18.957779 KDa
SequenceString:
MAATVLCGVL RAWRTGVPLG CGLRRLSQTQ GTPEYPSFVE SVDEYHFVER LLPPASIPRP PKHEHYPTPS GWQPPRDPAP SLPYFVRRS RMHNIPVYRD ITHGNRQMTV IRKVEGDIWA LQKDVEDFLS PLLGKTPVTQ VNEVTGTLRV KGYFDQQLKA W LLEKGF

+
Macromolecule #45: Mitochondrial ribosomal protein L50

MacromoleculeName: Mitochondrial ribosomal protein L50 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 18.351 KDa
SequenceString:
MAARWVSGLA RRSLTCAVSG APRREFWSPF RKEKQPVVAE TVEEVKKEPI LVCPPIQSRT YIPPEDLQSR LESHVKEVFG SSVPSNWQD ISLEDVHLKF SFLARLANDL RHAVPNSRLH QMCRVRDVLD FYNVPVQDRS KFDELIASNL PHNLKITWGY

+
Macromolecule #46: Mitochondrial ribosomal protein L51

MacromoleculeName: Mitochondrial ribosomal protein L51 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 15.249048 KDa
SequenceString:
MAGSLSWVTG RGLWGRLPLT CRSFSLGIPR LFHVRVTLPP RKVVDRWNEK RAMFGVYDNI GILGNFEKHP KELIKGPIWL RGWKGNELQ RCIRKKRMVG NRMFIDDLHN LNKRISYLYK HFNRHGKYR

+
Macromolecule #47: Mitochondrial ribosomal protein L52

MacromoleculeName: Mitochondrial ribosomal protein L52 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 13.813705 KDa
SequenceString:
MAAWGILLST GVRRLHCGTA AQAGSQWRLQ QGFAANPSGY GPLTELPDWS YADGRPAPPM KGQLRRKAQR EKFARRVVLL SQEMDAGLQ AWQLRQQEKL QEEEGKQKNA LKSKGALLQN PQPSQ

+
Macromolecule #48: mL53, MRPL53

MacromoleculeName: mL53, MRPL53 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 12.015747 KDa
SequenceString:
MAAALARLGL RAVKQVRVQF CPFEKNVEST RTFLQAVSSE KVRCTNLNCS VIADVRHDGS EPCVDVLFGD GHRLIMRGAH LTAQEMLTA FASHIQARGA AGSGDKPSAS TGR

+
Macromolecule #49: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 49
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 15.957357 KDa
SequenceString:
MAARRLFGAA RSWAAWRAWE LSDAAVSGRL HVRNYAKRPV IKGGKGGKGA VVGEALKDPE VCTDPFRLTT HAMGVNIYKE GQDVVLKPD SEYPEWLFEM NVGPPKKLEE LDPETREYWR LLRKHNIWRH NRLSKNRKF

+
Macromolecule #50: Mitochondrial ribosomal protein L57

MacromoleculeName: Mitochondrial ribosomal protein L57 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 12.001915 KDa
SequenceString:
MFLTALLRRN RIPGRQWIGK HRRPRPVSAQ AKQNMIRRLE TEAENQYWLS RPFLTAEQER GHAAVRRAAA FQALKAAQAA RFPAHRRLE EQLGHLLVTR KWS

+
Macromolecule #51: Mitochondrial ribosomal protein L58

MacromoleculeName: Mitochondrial ribosomal protein L58 / type: protein_or_peptide / ID: 51
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 23.545873 KDa
SequenceString: MAAARCLRWG LNRAGAWLLP SPTRYPRRAL HKQVEGTEFQ SIYSLDKLYP ESRGSDTAWR LPDDAKQAND IPVDRLTISY CRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRLKLA VKHKNRINRS GELILTSECS RYQFRNLADC LQKLRDMIAE A SQPAKEPS ...String:
MAAARCLRWG LNRAGAWLLP SPTRYPRRAL HKQVEGTEFQ SIYSLDKLYP ESRGSDTAWR LPDDAKQAND IPVDRLTISY CRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRLKLA VKHKNRINRS GELILTSECS RYQFRNLADC LQKLRDMIAE A SQPAKEPS REDAELRRSR IENMNRERLR KKRISSAIKT SRRVDVD

+
Macromolecule #52: 'Mitochondrial ribosomal protein L59

MacromoleculeName: 'Mitochondrial ribosomal protein L59 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 25.815031 KDa
SequenceString: MAAPVQQART LLRLVTTLGQ GSRGYRARPP PRRSQEPRWP DPDDPLTPRW QLSPRYAAKQ FARHGAASGV AAGSLWPSQE QLRELEAEE REWYPSLAAM QESLRVQQLA EEQKRQAREQ LIEECMAKMP QMIENWRQQQ QERREKEQAD KDRRARLQAE A QERLGYHV ...String:
MAAPVQQART LLRLVTTLGQ GSRGYRARPP PRRSQEPRWP DPDDPLTPRW QLSPRYAAKQ FARHGAASGV AAGSLWPSQE QLRELEAEE REWYPSLAAM QESLRVQQLA EEQKRQAREQ LIEECMAKMP QMIENWRQQQ QERREKEQAD KDRRARLQAE A QERLGYHV DPRSARFQEL LQDMEKQHRK RLKEEKQRKK KEARAAAMAA AAAQDPADSE TPSS

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Macromolecule #53: mL65, MRPS30

MacromoleculeName: mL65, MRPS30 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 49.100504 KDa
SequenceString: MAAARCWRFP LRGAGLSLHT AAKAAVTAPE VTGPDVPATP VARYPPIVAS LTAKSKAARQ RRVEQWQATV HAAKSVDEKL RILTKMQFM KYVVYPQTFA LNADNWYQSF TKTVFLSGLP PTPAKLEPEP TLDITALREA VCDCLLQEHF FLRRKKRAPV I QDREAIAS ...String:
MAAARCWRFP LRGAGLSLHT AAKAAVTAPE VTGPDVPATP VARYPPIVAS LTAKSKAARQ RRVEQWQATV HAAKSVDEKL RILTKMQFM KYVVYPQTFA LNADNWYQSF TKTVFLSGLP PTPAKLEPEP TLDITALREA VCDCLLQEHF FLRRKKRAPV I QDREAIAS PFLDQLVASL TGLLSVHNPV LAAAALDCKR PVHFFWLRGE EIIPRGHRKG RVDALRYQIN DKPHNQIRIS RQ LPEFVPL DYSIPIEVPV MSCKPDKLPL FKRQYENTIF IGSKTADPLC YGHTQFHLLP DKLKREKLLK QNCADQIEVV FRA NAIASL FAWTGAQAMY QGFWSEADVT RPFVSQGVIT DGKYFSFFCY QLNTLALTAQ ADQNNPRKNI CWGTQSKPLY ETIE DNNVK GFNDDVLLQL VQFLLNRPKE DKSQLLEN

+
Macromolecule #54: Mitochondrial ribosomal protein S18A

MacromoleculeName: Mitochondrial ribosomal protein S18A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 22.273084 KDa
SequenceString: MVGLNVLVSG CGRLLRGLLA GPAATSWARP PSRGFREVVE ILDGKTTIIE GRITETPQES PNPPNPTGQC PICRWNLKHK YNYEDVLLL SQFIRPHGGM LPRRITGLCQ EEHLKIEECV KMAHRAGLLP NHRPKLPEGF VPKSKPRLNR YLTRWSPRSV K PIYNKGHR ...String:
MVGLNVLVSG CGRLLRGLLA GPAATSWARP PSRGFREVVE ILDGKTTIIE GRITETPQES PNPPNPTGQC PICRWNLKHK YNYEDVLLL SQFIRPHGGM LPRRITGLCQ EEHLKIEECV KMAHRAGLLP NHRPKLPEGF VPKSKPRLNR YLTRWSPRSV K PIYNKGHR WNKVRMPVGS PLLKDNVSYS GRPLLLYH

+
Macromolecule #55: unassigned secondary structure elements

MacromoleculeName: unassigned secondary structure elements / type: protein_or_peptide / ID: 55
Details: Unassigned secondary structure elements have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 5.846387 KDa
SequenceString:
AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA

+
Macromolecule #12: 16S ribosomal RNA, mitochondrial

MacromoleculeName: 16S ribosomal RNA, mitochondrial / type: rna / ID: 12 / Number of copies: 1
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 504.852031 KDa
SequenceString: ACCAAAGCUA GCUCAACAUA CUAAACAAAU ACAAAAAUAC ACCAAAAUAA AAUAAAACAU UCACCUAACA UUAAAGUAUA GGAGAUAGA AAUUUUUAUC CUGACGCUAU AGAGAUAGUA CCGUAAGGGA AAGAUGAAAG AAUAAAAUAA AAGUAAAAAA A AGCAAAGA ...String:
ACCAAAGCUA GCUCAACAUA CUAAACAAAU ACAAAAAUAC ACCAAAAUAA AAUAAAACAU UCACCUAACA UUAAAGUAUA GGAGAUAGA AAUUUUUAUC CUGACGCUAU AGAGAUAGUA CCGUAAGGGA AAGAUGAAAG AAUAAAAUAA AAGUAAAAAA A AGCAAAGA UUACCCCUUC UACCUUUUGC AUAAUGGUUU AACCAGAAAA AAUCUAACAA AGAGAACUUU AGCUAGAUAC CC CGAAACC AGACGAGCUA CCCAUGAGCA GUUUAAAAGA ACCAACUCAU CUAUGUGGCA AAAUAGUGAG AAGACUUGUA GGU AGAGGU GAAAAGCCUA ACGAGCCUGG UGAUAGCUGG UUGUCCGAGA AAGAAUUUUA GUUCAACCUU AAAAAUACCC CAAA AACCC UAAAUUCCAA UGUAUUUUUA AGAGAUAGUC UAAAAAGGUA CAGCUUUUUA GAAACGGAUA CAACCUUGAC UAGAG AGUA AAAUCUUAAU ACUACCAUAG UAGGCCUAAA AGCAGCCAUC AAUUGAGAAA GCGUUAAAGC UCAACAAAUU CACCAA CAU AAUCCCAAAA ACUAAUAACA AACUCCUAGC CCAAUACCGG ACUAAUCUAU UGAAACAUAG AAGCAAUAAU GUUAAUA UG AGUAACAAGA AGCCUUUCUC CUCGCACACG CUUACAUCAG UAACUAAUAA UAUACUGAUA AUUAACAACC AAUAAACC A AAACAACACU AAAACGUUUA UUAAUUACAU UGUUAACCCA ACACAGGAGU GCACCAAGGA AAGAUUAAAA GAAGUAAAA GGAACUCGGC AAACACAAAC CCCGCCUGUU UACCAAAAAC AUCACCUCUA GCAUUACUAG UAUUAGAGGC AAUGCCUGCC CAGUGACAC CAGUUUAACG GCCGCGGUAU UCUGACCGUG CAAAGGUAGC AUAAUCACUU GUUCUCCAAA UAAGGACUUG U AUGAAUGG CCACACGAGG GUUUUACUGU CUCUUACUUC CAAUCAGUGA AAUUAACCUU CCCGUGAAGA GGCGGGAAUA AA AAAAUAA GACGAGAAGA CCCUAUGGAG CUUUAAUUAA CUAUUCCAAA AGUUAAACAA CUCAACCACA AAGGGAUAAA ACA UAACUU AACAUGGACU AGCAAUUUCG GUUGGGGUGA CCUCGGAGUA CAAAAAACCC UCCGAGUGAU UUUAAUCUAG ACAA ACCAG UCAAAAUAAC CAUAACAUCA CUUAUUGAUC CAAAAUUUUG AUCAACGGAA CAAGUUACCC UAGGGAUAAC AGCGC AAUC CUGUUCUAGA GUUCCUAUCG ACAAUAGGGU UUACGACCUC GAUGUUGGAU CAGGACACCC AAAUGGUGCA ACCGCU AUU AAAGGUUCGU UUGUUCAACG AUUAAAGUCC UACGUGAUCU GAGUUCAGAC CGGAGCAAUC CAGGUCGGUU UCUAUCU AU UAUAAAUUUC UCCCAGUACG AAAGGACAAG AGAAAUGGGA CCAACCUCAC AAACGCGUCU CAGAGAUAAU UAAUGAUU U AAUCUUAACC UAAUUAACUC AUAAUAAAUC CAGCCCUAGA ACAGGGCACA

+
Macromolecule #13: CP tRNAPhe, mitochondrial

MacromoleculeName: CP tRNAPhe, mitochondrial / type: rna / ID: 13 / Number of copies: 1
Source (natural)Organism: Sus scrofa (pig)
Molecular weightTheoretical: 23.402018 KDa
SequenceString:
GUUAAUGUAG CUUAAAUUAU CAAAGCAAGG CACUGAAAAU GCCUAGAUGA GCCUCACAGC UCCAUAAACA CCA

+
Macromolecule #56: P-site fMet-tRNAMet, mitochondrial

MacromoleculeName: P-site fMet-tRNAMet, mitochondrial / type: rna / ID: 56 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.664498 KDa
SequenceString:
AGUAAGGUCA GCUAAAUAAG CUAUCGGGCC CAUACCCCGA AAAUGUUGGU UAUACCCUUC CCGUACUACC A

+
Macromolecule #57: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 57 / Number of copies: 216 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #58: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 58 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #59: GUANOSINE-5'-MONOPHOSPHATE

MacromoleculeName: GUANOSINE-5'-MONOPHOSPHATE / type: ligand / ID: 59 / Number of copies: 2 / Formula: 5GP
Molecular weightTheoretical: 363.221 Da
Chemical component information

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE / Guanosine monophosphate

+
Macromolecule #60: SPERMINE

MacromoleculeName: SPERMINE / type: ligand / ID: 60 / Number of copies: 2 / Formula: SPM
Molecular weightTheoretical: 202.34 Da
Chemical component information

ChemComp-SPM:
SPERMINE / Spermine

+
Macromolecule #61: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

MacromoleculeName: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / type: ligand / ID: 61 / Number of copies: 1 / Formula: GSP
Molecular weightTheoretical: 539.246 Da
Chemical component information

ChemComp-GSP:
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

+
Macromolecule #62: SODIUM ION

MacromoleculeName: SODIUM ION / type: ligand / ID: 62 / Number of copies: 1
Molecular weightTheoretical: 22.99 Da

+
Macromolecule #63: N-FORMYLMETHIONINE

MacromoleculeName: N-FORMYLMETHIONINE / type: ligand / ID: 63 / Number of copies: 1 / Formula: FME
Molecular weightTheoretical: 177.221 Da
Chemical component information

ChemComp-FME:
N-FORMYLMETHIONINE / N-Formylmethionine

+
Macromolecule #64: water

MacromoleculeName: water / type: ligand / ID: 64 / Number of copies: 2 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration0.171 mg/mL
BufferpH: 7.6
GridModel: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
Detailscontains 55S mitochondrial ribosome, mitochondrial initiation factor 2, mitochondrial formyl-Met-tRNAMet and MT-CO3 mRNA

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number real images: 13936 / Average exposure time: 1.4 sec. / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 1366787
CTF correctionSoftware - Name: RELION (ver. 2.1)
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5aj4

Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final reconstructionNumber classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1) / Number images used: 75666

-
Atomic model buiding 1

RefinementSpace: RECIPROCAL / Protocol: OTHER / Overall B value: 56.9
Output model

PDB-6gb2:
Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 39S ribosomal subunit.

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