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- EMDB-20952: K.lactis 80S ribosome with p/PE tRNA and eIF5B -

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Basic information

Entry
Database: EMDB / ID: EMD-20952
TitleK.lactis 80S ribosome with p/PE tRNA and eIF5B
Map data
Sample
  • Complex: Kluyveromyces lactis 80S ribosome in complex with eIF5B
    • RNA: x 5 types
    • Protein or peptide: x 78 types
  • Ligand: x 2 types
Keywordstranslation / ribosome / initiation / eIF5B
Function / homology
Function and homology information


response to cycloheximide / 90S preribosome / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / cytosolic ribosome / translation initiation factor activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ...response to cycloheximide / 90S preribosome / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / cytosolic ribosome / translation initiation factor activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / protein kinase C binding / positive regulation of apoptotic signaling pathway / modification-dependent protein catabolic process / protein tag activity / rRNA processing / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosome biogenesis / ribosome binding / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / positive regulation of protein phosphorylation / ribonucleoprotein complex / translation / response to antibiotic / GTPase activity / mRNA binding / ubiquitin protein ligase binding / GTP binding / nucleolus / mitochondrion / RNA binding / zinc ion binding / nucleus / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / 50S ribosomal protein L10, insertion domain superfamily / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. ...Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / 50S ribosomal protein L10, insertion domain superfamily / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / : / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Ribosomal protein L18e / Ribosomal protein 60S L18 and 50S L18e / : / Ribosomal protein S26e signature. / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein L1, conserved site / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L1 signature. / Ribosomal protein L1 / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Small (40S) ribosomal subunit Asc1/RACK1 / S27a-like superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L44e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein S27a / Ribosomal protein L13e / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein L19, eukaryotic / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S30 / : / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S7e signature. / : / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein S19e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal protein L30e signature 1. / Ribosomal protein S3Ae signature. / Ribosomal_S19e / Ribosomal protein S27e signature. / Ribosomal L40e family / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain
Similarity search - Domain/homology
60S ribosomal protein L8 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein eL42 / Small ribosomal subunit protein eS28 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Ubiquitin-ribosomal protein eS31 fusion protein / KLLA0F25542p / KLLA0F23265p ...60S ribosomal protein L8 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein eL42 / Small ribosomal subunit protein eS28 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Ubiquitin-ribosomal protein eS31 fusion protein / KLLA0F25542p / KLLA0F23265p / KLLA0F18040p / Ribosomal protein L15 / Large ribosomal subunit protein uL3 / KLLA0F09812p / KLLA0F08261p / KLLA0F07843p / KLLA0F05247p / KLLA0F04675p / KLLA0F04499p / 40S ribosomal protein S12 / Small ribosomal subunit protein eS6 / KLLA0E23673p / KLLA0E23651p / 40S ribosomal protein S8 / Small ribosomal subunit protein uS2 / KLLA0E12453p / KLLA0E12277p / 40S ribosomal protein S27 / KLLA0E06997p / KLLA0E06843p / KLLA0E05941p / Small ribosomal subunit protein uS14 / KLLA0E03455p / 60S ribosomal protein L29 / KLLA0D16027p / 60S ribosomal protein L36 / KLLA0D10659p / 40S ribosomal protein S3 / KLLA0D07405p / KLLA0D06941p / KLLA0D05643p / KLLA0D05181p / 40S ribosomal protein S26 / KLLA0D03410p / KLLA0C18216p / 40S ribosomal protein S7 / KLLA0C08371p / 40S ribosomal protein S24 / 40S ribosomal protein S30 / Ribosomal protein L37 / KLLA0B13409p / KLLA0B08173p / Small ribosomal subunit protein uS8 / KLLA0B07139p / 40S ribosomal protein S25 / 60S acidic ribosomal protein P0 / KLLA0B05742p / Small ribosomal subunit protein eS1 / KLLA0B04686p / 40S ribosomal protein S4 / KLLA0B02937p / Ribosomal protein / KLLA0B01562p / KLLA0B01474p / KLLA0A10483p / KLLA0A07227p / KLLA0A07194p / KLLA0A06336p / Small ribosomal subunit protein eS21 / 60S ribosomal protein L13 / RPS23 / Small ribosomal subunit protein uS9 / RPL28 / Ubiquitin fusion protein
Similarity search - Component
Biological speciesKluyveromyces lactis (yeast) / Saccharomyces cerevisiae (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsFernandez IS / Huang BY
CitationJournal: Proc Natl Acad Sci U S A / Year: 2020
Title: Long-range interdomain communications in eIF5B regulate GTP hydrolysis and translation initiation.
Authors: Bridget Y Huang / Israel S Fernández /
Abstract: Translation initiation controls protein synthesis by regulating the delivery of the first aminoacyl-tRNA to messenger RNAs (mRNAs). In eukaryotes, initiation is sophisticated, requiring dozens of ...Translation initiation controls protein synthesis by regulating the delivery of the first aminoacyl-tRNA to messenger RNAs (mRNAs). In eukaryotes, initiation is sophisticated, requiring dozens of protein factors and 2 GTP-regulated steps. The GTPase eIF5B gates progression to elongation during the second GTP-regulated step. Using electron cryomicroscopy (cryo-EM), we imaged an in vitro initiation reaction which is set up with purified yeast components and designed to stall with eIF5B and a nonhydrolyzable GTP analog. A high-resolution reconstruction of a "dead-end" intermediate at 3.6 Å allowed us to visualize eIF5B in its ribosome-bound conformation. We identified a stretch of residues in eIF5B, located close to the γ-phosphate of GTP and centered around the universally conserved tyrosine 837 ( numbering), that contacts the catalytic histidine of eIF5B (H480). Site-directed mutagenesis confirmed the essential role that these residues play in regulating ribosome binding, GTP hydrolysis, and translation initiation both in vitro and in vivo. Our results illustrate how eIF5B transmits the presence of a properly delivered initiator aminoacyl-tRNA at the P site to the distant GTPase center through interdomain communications and underscore the importance of the multidomain architecture in translation factors to sense and communicate ribosomal states.
History
DepositionNov 14, 2019-
Header (metadata) releaseJan 15, 2020-
Map releaseJan 15, 2020-
UpdateMar 6, 2024-
Current statusMar 6, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.11
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.11
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6uz7
  • Surface level: 0.11
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_20952.png

Downloads & links

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Map

FileDownload / File: emd_20952.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 400 pix.
= 428. Å		1.07 Å/pix.
x 400 pix.
= 428. Å		1.07 Å/pix.
x 400 pix.
= 428. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.11 / Movie #1: 0.11
Minimum - Maximum-0.3795459 - 0.5876485
Average (Standard dev.)-0.00020349145 (±0.032959074)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 428.00003 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.071.071.07
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z428.000428.000428.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.3800.588-0.000

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Supplemental data

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Sample components

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Entire : Kluyveromyces lactis 80S ribosome in complex with eIF5B

EntireName: Kluyveromyces lactis 80S ribosome in complex with eIF5B
Components
  • Complex: Kluyveromyces lactis 80S ribosome in complex with eIF5B
    • RNA: 25S ribosomal RNA
    • RNA: RNA (121-MER)
    • RNA: 5.8S ribosomal RNA
    • Protein or peptide: KLLA0D16027p
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: KLLA0B07139p
    • Protein or peptide: KLLA0D06941p
    • Protein or peptide: KLLA0B04686p
    • Protein or peptide: KLLA0D03410p
    • Protein or peptide: KLLA0E00573p
    • Protein or peptide: KLLA0F04499p
    • Protein or peptide: KLLA0D05643p
    • Protein or peptide: KLLA0F08261p
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: KLLA0B13409p
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: KLLA0F04675p
    • Protein or peptide: KLLA0A06336p
    • Protein or peptide: KLLA0A07227p
    • Protein or peptide: KLLA0E12453p
    • Protein or peptide: 60S ribosomal protein L20
    • Protein or peptide: KLLA0E23651p
    • Protein or peptide: KLLA0D05181p
    • Protein or peptide: KLLA0E06997p
    • Protein or peptide: 60S ribosomal protein L24
    • Protein or peptide: 60S ribosomal protein L25
    • Protein or peptide: KLLA0B05742p
    • Protein or peptide: KLLA0E03455p
    • Protein or peptide: RPL28
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: KLLA0B02937p
    • Protein or peptide: KLLA0E06843p
    • Protein or peptide: KLLA0D07405p
    • Protein or peptide: KLLA0C08371p
    • Protein or peptide: KLLA0F05247p
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: KLLA0C18216p
    • Protein or peptide: 60S ribosomal protein L39
    • Protein or peptide: Ubiquitin fusion protein
    • Protein or peptide: 60S ribosomal protein L41
    • Protein or peptide: 60S ribosomal protein L44
    • Protein or peptide: KLLA0E05941p
    • Protein or peptide: Ribosomal protein
    • Protein or peptide: 60S acidic ribosomal protein P0
    • Protein or peptide: GDPCP
    • Protein or peptide: 40S ribosomal protein S0
    • Protein or peptide: 40S ribosomal protein S1
    • Protein or peptide: KLLA0F09812p
    • Protein or peptide: KLLA0D08305p
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: KLLA0D10659p
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: KLLA0E23673p
    • Protein or peptide: KLLA0B08173p
    • Protein or peptide: KLLA0A10483p
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: KLLA0F18040p
    • Protein or peptide: 40S ribosomal protein S14
    • Protein or peptide: KLLA0F07843p
    • Protein or peptide: 40S ribosomal protein S16
    • Protein or peptide: KLLA0B01474p
    • Protein or peptide: KLLA0B01562p
    • Protein or peptide: KLLA0A07194p
    • Protein or peptide: KLLA0F25542p
    • Protein or peptide: 40S ribosomal protein S21
    • Protein or peptide: 40S ribosomal protein S22
    • Protein or peptide: RPS23
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: 40S ribosomal protein S26
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: 40S ribosomal protein S28
    • Protein or peptide: 40S ribosomal protein S29
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
    • Protein or peptide: KLLA0E12277p
    • RNA: 18S ribosomal RNA
    • Protein or peptide: KLLA0F23265p
    • RNA: RNA (76-MER)
  • Ligand: ZINC ION
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

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Supramolecule #1: Kluyveromyces lactis 80S ribosome in complex with eIF5B

SupramoleculeName: Kluyveromyces lactis 80S ribosome in complex with eIF5B
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#83
Source (natural)Organism: Kluyveromyces lactis (yeast)

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Macromolecule #1: 25S ribosomal RNA

MacromoleculeName: 25S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 1.08698575 MDa
SequenceString: GUUUGACCUC AAAUCAGGUA GGAAUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGC GUCUUCGACG UCCGAGUUGU AAUUUGAAGA A GGCAACUU ...String:
GUUUGACCUC AAAUCAGGUA GGAAUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGC GUCUUCGACG UCCGAGUUGU AAUUUGAAGA A GGCAACUU UGUAGCUGGU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA UC CCAGUUA UUUGUAAAGU GCUUUCGACG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGCG UUUGCUUCGG CUUUCGCUGG GCCAGCAUCA GUUUU AGCG GUUGGAUAAA UCCUCGGGAA UGUGGCUCUG CUUCGGUAGA GUGUUAUAGC CCGUGGGAAU ACAGCCAGCU GGGACU GAG GAUUGCGACU UUUGUCAAGG AUGCUGGCGU AAUGGUUAAA UGCCGCCCGU CUUGAAACAC GGACCAAGGA GUCUAAC GU CUAUGCGAGU GUUUGGGUGU AAAACCCGUA CGCGUAAUGA AAGUGAACGU AGGUGAGGGC CCGCAAGGGU GCAUCAUC G ACCGAUCCUG AUGUCUUCGG AUGGAUUUGA GUAAGAGCAU AGCUGUUGGG ACCCGAAAGA UGGUGAACUA UGCCUGAAU AGGGUGAAGC CAGAGGAAAC UCUGGUGGAG GCUCGUAGCG GUUCUGACGU GCAAAUCGAU CGUCGAAUUU GGGUAUAGGG GCGAAAGAC UAAUCGAACC AUCUAGUAGC UGGUUCCUGC CGAAGUUUCC CUCAGGAUAG CAGAAGCUCG UAUCAGUUUU A UGAGGUAA AGCGAAUGAU UAGAGGUACC GGGGUUGAAA UGACCUUGAC CUAUUCUCAA ACUUUAAAUA UGUAAGAAGU CC UUGUUGC UUAAUUGAAC GUGGACAUUU GAAUGAAGAG CUUUUAGUGG GCCAUUUUUG GUAAGCAGAA CUGGCGAUGC GGG AUGAAC CGAACGUGGA GUUAAGGUGC CGGAAUACAC GCUCAUCAGA CACCACAAAA GGUGUUAGUU CAUCUAGACA GCCG GACGG UGGCCAUGGA AGUCGGAAUC CGCUAAGGAG UGUGUAACAA CUCACCGGCC GAAUGAACUA GCCCUGAAAA UGGAU GGCG CUCAAGCGUG UUACCUAUAC UCCACCGUCA GGGUUAAUAU GAUGCCCUGA CGAGUAGGCA GGCGUGGAGG UCAGUG ACG AAGCCUAGGC UGUAAAGCUG GGUAGAACGG CCUCUAGUGC AGAUCUUGGU GGUAGUAGCA AAUAUUCAAA UGAGAAC UU UGAAGACUGA AGUGGGGAAA GGUUCCACGU CAACAGCAGU UGGACGUGGG UUAGUCGAUC CUAAGAGAUG GGGAAGCU C CGUUUCAAAG GCCUAAUUUU CUAGGCCACC AUCGAAAGGG AAUCCGGUUA AUAUUCCGGA ACCUGGAUAU GGAUUCUUC ACGGUAACGU AACUGAAUGU GGAGACGUCG GCGCGAGCCC UGGGAGGAGU UAUCUUUUCU UCUUAACAGC UUAUCACCCC GGAAUUGGU UUAUCCGGAG AGGGGGUCUU AUGGCUGGAA GAGCCCAGCC CUUGUGCUGG GUCCGGUGCG CCCGCGACGG C CCUUGAAA AUCCACAGGA AGGAAUAGUU UUCAUGCCAG GUCGUACUGA UAACCGCAGC AGGUCUCCAA GGUGAACAGC CU CUAGUUG AUAGAAUAAU GUAGAUAAGG GAAGUCGGCA AAAUAGAUCC GUAACUUCGG GAUAAGGAUU GGCUCUAAGG AUC GGGAAG UGAGGGCCUU GGUCAGACGC GGCGGGCAUG CUUGUGGACU GUCUUACUGG GCUUGCUCGG UGGGACGGAC UGCU UGCGG GCCUUGUUGU AGACGGCCUU GGUAGGUCUC UUGUAGAUCG UCGCUUGCUG CAAUUAACGA UCAACUUAGA ACUGG UACG GACAAGGGGA AUCUGACUGU CUAAUUAAAA CAUAGCAUUG CGAUGGUCAG AAAGUGAUGU UGACGCAAUG UGAUUU CUG CCCAGUGCUC UGAAUGUCAA AGUGAAGAAA UUCAACCAAG CGCGGGUAAA CGGCGGGAGU AACUAUGACU CUCUUAA GG UAGCCAAAUG CCUCGUCAUC UAAUUAGUGA CGCGCAUGAA UGGAUUAACG AGAUUCCCAC UGUCCCUAUC UACUAUCU A GCGAAACCAC AGCCAAGGGA ACGGGCUUGG CAGAAUCAGC GGGGAAAGAA GACCCUGUUG AGCUUGACUC UAGUUUGAC AUUGUGAAGA GACAUAGAGG GUGUAGCAUA AGUGGGAGCU UCGGCGCCAG UGAAAUACCA CUACCUUUAU AGUUUCUUUA CUUAUUCAA UUAAGCGGAG CUGGAAUUCA UUUUCCACGU UCUAGCAUUC AAAGUCCUAU ACGGGCUGAU CCGGGUUGAA G ACAUUGUC AGGUGGGGAG UUUGGCUGGG GCGGCACAUC UGUUAAACGA UAACGCAGAU GUCCUAAGGG GGACUCAUGG AG AACAGAA AUCUCCAGUA GAACAAAAGG GUAAAAGUCC CCUUGAUUUU GAUUUUCAGU GUGAAUACAA ACCAUGAAAG UGU GGCCUA UCGAUCCUUU AGGUCCUCGG AAUUUGAGGC UAGAGGUGCC AGAAAAGUUA CCACAGGGAU AACUGGCUUG UGGC AGUCA AGCGUUCAUA GCGACAUUGC UUUUUGAUUC UUCGAUGUCG GCUCUUCCUA UCAUACCGAA GCAGAAUUCG GUAAG CGUU GGAUUGUUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGUCGUG AGACAGGUUA GUUUUACCCU ACUGAU GAA UGUUAUCGCA AUAGUAAUUG AACUUAGUAC GAGAGGAACA GUUCAUUCGG AUAAUUGGUU UUUGCGGCUG UCUGAUC AG GCAUUGCCGC GAAGCUACCA UCCGCUGGAU UAUGGCUGAA CGCCUCUAAG UCAGAAUCCA UGCUAGAACG CGAUGAUU U CUUUGCCUUG CACAAUAUAG AAGGAUAAGA AUAAGGCGUC UUUUUGGCGU CGCUGAACCA UAGCAGGCUG GCAACGGUG CUCUUAGCGG AAAGGCUUUG GGUGCUUGCC GGCGAAUUGC AAUGUCAUUU UGCGCAAGGA UAAAUCAUUU GUAUACGACU UAAAUGUAC AACAGGGUAU UGUAAGCAGU AGAGUAGCCU UGUUGUUACG AUCUGCUGAG AUUAAGCCUU CGUUGUCUGA U UUGU

GENBANK: GENBANK: XR_002633589.1

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Macromolecule #2: RNA (121-MER)

MacromoleculeName: RNA (121-MER) / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 38.951105 KDa
SequenceString:
GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

GENBANK: GENBANK: CP021242.1

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Macromolecule #3: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 910.658562 KDa
SequenceString: UCAUUCAAAU UUCUGCCCUA UCAACUUUCG AUGGUAGGAU AGUGGCCUAC CAUGGUUUCA ACGGGUAACG GGGAAUAAGG GUUCGAUUC CGGAGAGGGA GCCUGAGAAA CGGCUACCAC AUCCAAGGAA GGCAGCAGGC GCGCAAAUUA CCCAAUCCUA A UUCAGGGA ...String:
UCAUUCAAAU UUCUGCCCUA UCAACUUUCG AUGGUAGGAU AGUGGCCUAC CAUGGUUUCA ACGGGUAACG GGGAAUAAGG GUUCGAUUC CGGAGAGGGA GCCUGAGAAA CGGCUACCAC AUCCAAGGAA GGCAGCAGGC GCGCAAAUUA CCCAAUCCUA A UUCAGGGA GGUAGUGACA AUAAAUAACG AUACAGGGCC CAUUCGGGUC UUGUAAUUGG AAUGAGUACA AUGUAAAUAC CU UAACGAG GAACAACUGG AGGGCAAGUC UGGUGCCAGC AGCCGCGGUA AUUCCAGCUC CAGUAGCGUA UAUUAAAGUU GUU GCAGUU AAAAAGCUCG UAGUUGAACU UUGGGUCUGG UUGUCCGGUC CGACUUUAUG UCGCGCACUG GUUUUCAACC GGAU CUUUC CUUCUGGCUA ACCUGUACUC CUUGUGGGUG CAGGCGAACC AGGACUUUUA CUUUGAAAAA AUUAGAGUGU UCAAA GCAG GCGAAAGCUC GAAUAUAUUA GCAUGGAAUA AUGGAAUAGG ACGUUUGGUU CUAUUUUGUU GGUUUCUAGG ACCAUC GUA AUGAUUAAUA GGGACGGUCG GGGGCAUCAG UAUUCAAUUG UCAGAGGUGA AAUUCUUGGA UUUAUUGAAG ACUAACU AC UGCGAAAGCA UUUGCCAAGG ACGUUUUCAU UAAUCAAGAA CGAAAGUUAG GGGAUCGAAG AUGAUCAGAU ACCGUCGU A GUCUUAACCA UAAACUAUGC CGACUAGGGA UCGGGUGGUG UUUUUCUUAU GACCCACUCG GCACCUUACG AGAAAUCAA AGUCUUUGGG UUCUGGGGGG AGUAUGGUCG CAAGGCUGAA ACUUAAAGGA AUUGACGGAA GGGCACCACC AGGAGUGGAG CCUGCGGCU UAAUUUGACU CAACACGGGG AAACUCACCA GGUCCAGACA CAAUAAGGAU UGACAGAUUG AGAGCUCUUU C UUGAUUUU GUGGGUGGUG GUGCAUGGCC GUUCUUAGUU GGUGGAGUGA UUUGUCUGCU UAAUUGCGAU AACGAACGAG AC CUUAACC UACUAAAUAG GGUUGCUGGC ACUUGCCGGU UGACUCUUCU UAGAGGGACU AUCGGUUUCA AGCCGAUGGA AGU UUGAGG CAAUAACAGG UCUGUGAUGC CCUUAGACGU UCUGGGCCGC ACGCGCGCUA CACUGACGGA GCCAGCGAGU ACAA CCUUG GCCGAGAGGU CUGGGUAAUC UUGUGAAACU CCGUCGUGCU GGGGAUAGAG CAUUGUAAUU AUUGCUCUUC AACGA GGAA UUCCUAGUAA GCGCAAGUCA UCAGCUUGCG UUGAUUACGU CCCUGCCCUU UGUACACACC GCCCGUCGCU AGUACC GAU UGAAUGGCUU AGUGAGGCCU CAGGAUUUGC UUAGAGAAGG GGGCAACUCC AUCUCAGAGC GAAGAAUCUG GUCAAAC UU GGUCAUUUAG AGGAACUAAA AGUCGUAACA AGGUUUCCGU AGGUGAACCU GCGGAAGGAU CAUUAAAGAU UAUGAAUG A GUAGAUUACU GGGGGAAUCG CUGAAUAUGG CCUGCGCUUA AUUGCGCGGC UAAUUCUUGA UUUUCUGCUA UCAGUUUUC UUUCUCUCAU CCUAAACACA AUGGAGUUUU UUCUCUAUGA ACUACUUCCC UGGAGAGCUC GUCUCUCCAG UGGACAUAAA CACAAACAA CAUUUUGCAU UAUGAAAAAC UAUUUAUCAA GAAAUUUAAU AUUCAAAACU UUCAACAACG GAUCUCUUGG U UCUCGCAU CGAUGAAGAA CGCAGCGAAU UGCGAUAUGU AUUGUGAAUU GCAGAUUUUC GUGAAUCAUC AAAUCUUUGA AC GCACAUU GCGCCCUCUG GUAUUCCAGG GGGCAUGCCU GUUUGAGCGU CAUUUCUCUC UCAAACCUUU GGGUUUGGUA GUG AGUGAU ACUCGUUUUU CGGGUUAACU UGAAAGUGGC UAGCCGUUGC CUUCUGCGUG AGCAGGGCUG CGUGUCAAGU CUAU GGACU CGACUCUUGC ACAUCUACGU CUUAGGUUUG CGCCAAUUCG UGGUAAGCUA GGGUCAAUGA GCUUAUAGGU GUUAU AAAG ACUCGCUGGU GUUUGUCUCC UUGAGGCAUA CGGCUUAAUC AAAACUCUCA AAGUUUGACC UCAAAUCAGG UAGGAA UAC CCGCUGAACU UAAGCAUAUC AAUAAGCGGA GGAAAAGAAA CCAACCGGGA UUGCCUUAGU AACGGCGAGU GAAGCGG CA AAAGCUCAAA UUUGAAAUCU GGCGUCUUCG ACGUCCGAGU UGUAAUUUGA AGAAGGCAAC UUUGUAGCUG GUCCUUGU C UAUGUUCCUU GGAACAGGAC GUCAUAGAGG GUGAGAAUCC CGUGUGGCGA GGAUCCCAGU UAUUUGUAAA GUGCUUUCG ACGAGUCGAG UUGUUUGGGA AUGCAGCUCU AAGUGGGUGG UAAAUUCCAU CUAAAGCUAA AUAUUGGCGA GAGACCGAUA GCGAACAAG UACAGUGAUG GAAAGAUGAA AAGAACUUUG AAAAGAGAGU GAAAAAGUAC GUGAAAUUGU UGAAAGGGAA G GGCAUUUG AUCAGACAUG GCGUUUGCUU CGGCUUUCGC UGGGCCAGCA UCAGUUUUAG CGGUUGGAUA AAUCCUCGGG AA UGUGGCU CUGCUUCGGU AGAGUGUUAU AGCCCGUGGG AAUACAGCCA GCUGGGACUG AGGAUUGCGA CUUUUGUCAA GGA UGCUGG CGUAAUGGUU AAAUGCCGCC CGUCUUGAAA CACGGACCAA GGAGUCUAAC GUCUAUGCGA GUGUUUGGGU GUAA AACCC GUACGCGUAA UGAAAGUGAA

GENBANK: GENBANK: CP021242.1

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Macromolecule #81: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 81 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 579.239625 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAAUUC U AGAGCUAA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAAUUC U AGAGCUAA UACAUGCUUA AAAUCUCGAC CCUUUGGAAG AGAUGUAUUU AUUAGAUAAA AAAUCAAUGU CUUCGGACUC CU UGAUGAU UCAUAAUAAC UUUUCGAAUC GCAUGGCCUU GUGCUGGCGA UGGUUCAUUC AAAUUUCUGC CCUAUCAACU UUC GAUGGU AGGAUAGUGG CCUACCAUGG UUUCAACGGG UAACGGGGAA UAAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAA CGGCU ACCACAUCCA AGGAAGGCAG CAGGCGCGCA AAUUACCCAA UCCUAAUUCA GGGAGGUAGU GACAAUAAAU AACGA UACA GGGCCCAUUC GGGUCUUGUA AUUGGAAUGA GUACAAUGUA AAUACCUUAA CGAGGAACAA CUGGAGGGCA AGUCUG GUG CCAGCAGCCG CGGUAAUUCC AGCUCCAGUA GCGUAUAUUA AAGUUGUUGC AGUUAAAAAG CUCGUAGUUG AACUUUG GG UCUGGUUGUC CGGUCCGACU UUAUGUCGCG CACGGUUUUU CAACCGGAUC UUUCCUUCUG GCUAACCUGU ACUCCUUG U GGGUGCAGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGAAA GCUCGAAUAU AUUAGCAUG GAAUAAUGGA AUAGGACGUU UGGUUCUAUU UUGUUGGUUU CUAGGACCAU CGUAAUGAUU AAUAGGGACG GUCGGGGGCA UCAGUAUUC AAUUGUCAGA GGUGAAAUUC UUGGAUUUAU UGAAGACUAA CUACUGCGAA AGCAUUUGCC AAGGACGUUU U CAUUAAUC AAGAACGAAA GUUAGGGGAU CGAAGAUGAU CAGAUACCGU CGUAGUCUUA ACCAUAAACU AUGCCGACUA GG GAUCGGG UGGUGUUUUU CUUAUGACCC ACUCGGCACC UUACGAGAAA UCAAAGUCUU UGGGUUCUGG GGGGAGUAUG GUC GCAAGG CUGAAACUUA AAGGAAUUGA CGGAAGGGCA CCACCAGGAG UGGAGCCUGC GGCUUAAUUU GACUCAACAC GGGG AAACU CACCAGGUCC AGACACAAUA AGGAUUGACA GAUUGAGAGC UCUUUCUUGA UUUUGUGGGU GGUGGUGCAU GGCCG UUCU UAGUUGGUGG AGUGAUUUGU CUGCUUAAUU GCGAUAACGA ACGAGACCUU AACCUACUAA AUAGGGUUGC UGGCAC UUG CCGGUUGACU CUUCUUAGAG GGACUAUCGG UUUCAAGCCG AUGGAAGUUU GAGGCAAUAA CAGGUCUGUG AUGCCCU UA GACGUUCUGG GCCGCACGCG CGCUACACUG ACGGAGCCAG CGAGUACAAC CUUGGCCGAG AGGUCUGGGU AAUCUUGU G AAACUCCGUC GUGCUGGGGA UAGAGCAUUG UAAUUAUUGC UCUUCAACGA GGAAUUCCUA GUAAGCGCAA GUCAUCAGC UUGCGUUGAU UACGUCCCUG CCCUUUGUAC ACACCGCCCG UCGCUAGUAC CGAUUGAAUG GCUUAGUGAG GCCUCAGGAU UUGCUUAGA GAAGGGGGCA ACUCCAUCUC AGAGCGAAGA AUCUGGUCAA ACUUGGUCAU UUAGAGGAAC UAAAAGUCGU A ACAAGGUU UCCGUAGGUG AACCUGCGGA AGGAUCAUUA

GENBANK: GENBANK: CR382124.1

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Macromolecule #83: RNA (76-MER)

MacromoleculeName: RNA (76-MER) / type: rna / ID: 83 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 24.532604 KDa
SequenceString:
GCCCGGAUAG CUCAGAAGGU AGAGCAGGGG AUUGAAAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA

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Macromolecule #4: KLLA0D16027p

MacromoleculeName: KLLA0D16027p / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 27.448518 KDa
SequenceString: MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR NLDYAERHGY IRGVVKQIVH DAGRGAPLAK VVFRDPYKYK LREETFIANE GLHTGQFIY AGKKASLNVG NVLPLGSVPE GTIISNVEER PGDRGALARA SGNYVIVIGH NPEDNKTRVR LPSGAKKIIS S DARGVIGV ...String:
MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR NLDYAERHGY IRGVVKQIVH DAGRGAPLAK VVFRDPYKYK LREETFIANE GLHTGQFIY AGKKASLNVG NVLPLGSVPE GTIISNVEER PGDRGALARA SGNYVIVIGH NPEDNKTRVR LPSGAKKIIS S DARGVIGV VAGGGRIDKP LLKAGRAFHK YRVKRNSWPK TRGVAMNPVD HPHGGGNHQH IGKASTISRG AVSGQKAGLI AA RRTGLLR GSQKTQD

UniProtKB: KLLA0D16027p

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Macromolecule #5: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 43.7815 KDa
SequenceString: MSHRKYEAPR HGHLGFLPRK RAASVRGRVK SFPKDDQTKP VALTSFLGYK AGMSTIVRDL DRPGSKFHKR EVVEAVTVVD TPPIVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAE NGAQVDRELA RIKKYASVVR V LVHTQVRK ...String:
MSHRKYEAPR HGHLGFLPRK RAASVRGRVK SFPKDDQTKP VALTSFLGYK AGMSTIVRDL DRPGSKFHKR EVVEAVTVVD TPPIVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAE NGAQVDRELA RIKKYASVVR V LVHTQVRK TPLSQKKAHL AEIQLNGGSV SDKVDWAKEH FEKTVAVDSV FEQNEMIDVV AVTKGHGFEG VTHRWGTKKL PR KTHRGLR KVACIGAWHP AHVMWSVARA GQRGYHHRTS INHKVYRVGK GDDEANAATE FDRTKKTITP MGGFVHYGAI NND FVILKG SIPGTRKRVV TLRKSLYTNT SRKALEEVTL KWIDTASKFG KGRFQTPAEK HAFLGTLKKD L

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #6: KLLA0B07139p

MacromoleculeName: KLLA0B07139p / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 39.283227 KDa
SequenceString: MSIPQVAVLS LNGETTSTDL PLPAVFRAPI RPDIVHSVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTHRSGQAA FGNMCRGGRM FAPTKTWRKW NVKVNQNEKR YATASAIAAS AVPSLVLARG HRVEKVPSIP LVVSDDLQSI Q KTKEAVAA ...String:
MSIPQVAVLS LNGETTSTDL PLPAVFRAPI RPDIVHSVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTHRSGQAA FGNMCRGGRM FAPTKTWRKW NVKVNQNEKR YATASAIAAS AVPSLVLARG HRVEKVPSIP LVVSDDLQSI Q KTKEAVAA LKAVGAHSDV IKVVKSKKLR AGKGKYRNRR FTQRRGPLVV YSEDNGVVKA FRNVPGVETA NVASLGLLQL AP GAHLGRF VIWTQSAFSK LDQIWGSETV ASVKAGYALP SNIISTSDVT RIINSTEIQS VLRAAGQATQ KRTHVQKKNP LKN KQVLLR LNPYAKVFAA EKLGSKKVDQ ANKIKPSATA LETLKHD

UniProtKB: KLLA0B07139p

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Macromolecule #7: KLLA0D06941p

MacromoleculeName: KLLA0D06941p / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 33.937078 KDa
SequenceString: MAFIKDIKTS AYHSRYQTPF RRRREGKTDY YARKRLVAQH KAKYNTPKYR LVVRFTNKDI ITQIVSSTIT GDIVLAAAYS HELPRYGIT HGLTNWSAAY ATGLLLARRT LQKLGLDETY PGVEEVEGEY ELTEAVEDGP RPFKVYLDVG LQRTTTGARI F GALKGASD ...String:
MAFIKDIKTS AYHSRYQTPF RRRREGKTDY YARKRLVAQH KAKYNTPKYR LVVRFTNKDI ITQIVSSTIT GDIVLAAAYS HELPRYGIT HGLTNWSAAY ATGLLLARRT LQKLGLDETY PGVEEVEGEY ELTEAVEDGP RPFKVYLDVG LQRTTTGARI F GALKGASD GGLYVPHSEN RFPGWDFEAE ELDADLLRTY IFGGHVSQYM EELADDDEER YRELFKGYLA DDIDADAVED IY ADAHAAI REDPAFKPTE KKFTKEQYAA ESKKYRQVKL TREERKARVA AKISALAPQE

UniProtKB: KLLA0D06941p

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Macromolecule #8: KLLA0B04686p

MacromoleculeName: KLLA0B04686p / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 20.022352 KDa
SequenceString:
MAQAQKWYPA EEVPAPKQTR KAARPQKLRA SLVPGTVLIL LAGRFRGKRV VYLKHLEDNT LLVSGPFKVN GVPLRRVNAR YVIATSTRV SLEGVNTEKF NVAYFSREKL SRKQRAEANF FNEDQPKKEI KAERIEDQKN IDKALLAEIK KTPLLKQYLA A TFSLKNGD RPHLLKF

UniProtKB: KLLA0B04686p

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Macromolecule #9: KLLA0D03410p

MacromoleculeName: KLLA0D03410p / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 27.127383 KDa
SequenceString: MSASKVLVPE SQVKKAKAQQ KTAEQVAAER VARKAEKRAA ILERNAAYQK EYETAERAVI DAKREAKANS SYYVSAEAKL VFVVRIKGI NKIPPKPRKV LQLLRLNQIN SGTFVKVTKA TSELLKLIEP YVAYGYPSYS TVRQLVYKRG YGKINKQRIP L SDNSIVEA ...String:
MSASKVLVPE SQVKKAKAQQ KTAEQVAAER VARKAEKRAA ILERNAAYQK EYETAERAVI DAKREAKANS SYYVSAEAKL VFVVRIKGI NKIPPKPRKV LQLLRLNQIN SGTFVKVTKA TSELLKLIEP YVAYGYPSYS TVRQLVYKRG YGKINKQRIP L SDNSIVEA NLGNYGILSV DDLIHEIVTV GPHFKQANNF LWPFKLSNPS GGWGVPRKFK HFIQGGSFGN REEFINKLVK SM N

UniProtKB: KLLA0D03410p

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Macromolecule #10: KLLA0E00573p

MacromoleculeName: KLLA0E00573p / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 28.08092 KDa
SequenceString: MAPSKKVAPA PLASKAASAK KSNPLTKSSP RNFGIGQAIQ PKRNLSRYVK WPEYVRLQRQ KKILSIRLKV PPSIAQFQNT LDRNTAAET FKLLKKYSPE TAAEKKERLT KEAAAIAEGK TKQEASPKPY VVKYGLNHVV SLIENKKAKL VLIANDVDPI E LVIFLPAL ...String:
MAPSKKVAPA PLASKAASAK KSNPLTKSSP RNFGIGQAIQ PKRNLSRYVK WPEYVRLQRQ KKILSIRLKV PPSIAQFQNT LDRNTAAET FKLLKKYSPE TAAEKKERLT KEAAAIAEGK TKQEASPKPY VVKYGLNHVV SLIENKKAKL VLIANDVDPI E LVIFLPAL CKKMGVPYAI VKGKARLGTL VNQKTSAVAA LTEVREEDEA ALAKLVSTIN ANYLEKYEDS KKHWGGGIMG QK AQKKIEK RAKAAESA

UniProtKB: 60S ribosomal protein L8

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Macromolecule #11: KLLA0F04499p

MacromoleculeName: KLLA0F04499p / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 21.730055 KDa
SequenceString:
MKYIQTDQVL DVPEGVTVQI RSRIIKVTGP RGVLTKNLKH IDVTFTKVSN RQIKITVHNG DRKHVAALRT VKSLISNLIT GVTKGYKYK LRYVYAHFPI NVNVIEKDGA KFIEIRNFLG DKKVREVPVR EGVSVDFSTN QKDEIVLSGN SVENVSQNAA D IQQICRVR NKDIRKFLDG IYVSEKGLIE EEE

UniProtKB: KLLA0F04499p

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Macromolecule #12: KLLA0D05643p

MacromoleculeName: KLLA0D05643p / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 25.400488 KDa
SequenceString: MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL SSEALEAARI TANKYITKMT GRDSFHLRV RVHPFHVLRI NKMLSCAGAD RLQQGMRGAW GKPHGLAARV AIGQVIFSVR TKDNNKDTVI EGLRRARYKF P GQQKIIIS ...String:
MARRPARCYR YQKNKPYPKS RYNRAVPDSK IRIYDLGKKK ATVDEFPLCV HLVSNELEQL SSEALEAARI TANKYITKMT GRDSFHLRV RVHPFHVLRI NKMLSCAGAD RLQQGMRGAW GKPHGLAARV AIGQVIFSVR TKDNNKDTVI EGLRRARYKF P GQQKIIIS KKWGFTSLNR EEYVKKRDAG EIKDDGAFVK FLSKKGSLEE NIREFPEYFA KA

UniProtKB: KLLA0D05643p

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Macromolecule #13: KLLA0F08261p

MacromoleculeName: KLLA0F08261p / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 19.821848 KDa
SequenceString:
MSAKAQNPMR ELKIEKLVLN ISVGESGDRL TRASKVLEQL SGQTPVQSKA RYTVRTFGIR RNEKIAVHVT VRGPKAEEIL ERGLKVKEY QLRNRNFSAT GNFGFGIQEH IDLGIKYDPG IGIYGMDFYI VMGRPGARVT RRKRCQSKIG NSHKTSKEDT I AWFKQRYD AEVLDK

UniProtKB: KLLA0F08261p

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Macromolecule #14: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 22.702346 KDa
SequenceString: MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRHARA SKAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEI KAAGLTPAY ARTIGIAVDH RRQNRNQEIL DLNIQRLKEY QSKIIVFPRK GKAPEAEQTL SAAASFPIVQ PVTDSETRAV E DNGESAYR ...String:
MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRHARA SKAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEI KAAGLTPAY ARTIGIAVDH RRQNRNQEIL DLNIQRLKEY QSKIIVFPRK GKAPEAEQTL SAAASFPIVQ PVTDSETRAV E DNGESAYR TLRLARSDKR YKGIREKRAK DKAEAEAEKK K

UniProtKB: 60S ribosomal protein L13

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Macromolecule #15: KLLA0B13409p

MacromoleculeName: KLLA0B13409p / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.037637 KDa
SequenceString:
MSTDSVVKAS NWRLVEVGRV VLVKQGQSAG KLATIVEIID QKRALIDGPT TGVPRQAINL GQVVLTPLTF AVPRGAKSAT VSKKWAAAG VSEKWATSSW AKKIAQRERR AALSDFERFQ VLVLKKQKRY AVKKAVAKA

UniProtKB: KLLA0B13409p

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Macromolecule #16: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 24.454307 KDa
SequenceString: MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAS RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVSKGAT YGKPTNQGV NQLKYQRSLR ATAEERVGRR AANLRVLNSY WINQDSTYKY FEVILVDPSH KAIRRDAKYN WICNPVHKHR E ARGLTATG ...String:
MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAS RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVSKGAT YGKPTNQGV NQLKYQRSLR ATAEERVGRR AANLRVLNSY WINQDSTYKY FEVILVDPSH KAIRRDAKYN WICNPVHKHR E ARGLTATG KKSRGINKGH KFHNTQAGRR KTWKRQNTLS LWRYRK

UniProtKB: Ribosomal protein L15

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Macromolecule #17: KLLA0F04675p

MacromoleculeName: KLLA0F04675p / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 22.385344 KDa
SequenceString: MSSFEPVVVI DGKGHLLGRL ASTVAKQLLN GQKIVVVRAE ALNISGEFFR NKLKYHDYLR KATAFNKTRG PFHFRAPSRI FYKAVRGMI SHKTARGKAA LERLKVFEGV PPPYDKKKRV VVPQALRVLR LKPGRKYTTL GKLSTSVGWK YEDVVAKLEE K RKARSAEY ...String:
MSSFEPVVVI DGKGHLLGRL ASTVAKQLLN GQKIVVVRAE ALNISGEFFR NKLKYHDYLR KATAFNKTRG PFHFRAPSRI FYKAVRGMI SHKTARGKAA LERLKVFEGV PPPYDKKKRV VVPQALRVLR LKPGRKYTTL GKLSTSVGWK YEDVVAKLEE K RKARSAEY YLKKKAYNAV VANKSATVGT EVSEKLAALG Y

UniProtKB: KLLA0F04675p

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Macromolecule #18: KLLA0A06336p

MacromoleculeName: KLLA0A06336p / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 20.696502 KDa
SequenceString:
MARYGATSTN PAKSASARGS YLRVSYKNTR ETAQAISGWN LQKAQKYLDQ VLDHQRAIPF RRFNSSIGRT AQGKEFGVTK ARWPAKSVK FVQGLLQNAA SNAEAKGLDA TRLYVSHIQV NQAPKQRRRT FRAHGRINKY ESSPSHIELV VTEKEEAVEK A SEKKVARL SSRQRGRLAT QKRITA

UniProtKB: KLLA0A06336p

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Macromolecule #19: KLLA0A07227p

MacromoleculeName: KLLA0A07227p / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 20.668191 KDa
SequenceString:
MGIDHTSKQH KRSSHRTAPK SDNVYLKLLV KLYAFLARRT DAPFNKVILK SLFLSKINRP PVSVSRISRA LKQEGAAQKT IVVVGTVTD DNRIFEFPKT TVAALRFTAG AKAKILKAGG EAITLDQLAV RAPKGQNTLI VRGPRASREA ARHFGFGPHK G KAPRIQST GRKFERARGR RRSRAFKV

UniProtKB: KLLA0A07227p

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Macromolecule #20: KLLA0E12453p

MacromoleculeName: KLLA0E12453p / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 21.81948 KDa
SequenceString:
MANLRTQKRL AASVAGVGKR KIWMDPSETA EIAQANSRNA IRKLVKNGTI VKKPTRIHSR SRTRAMAESK RNGRHTGYGK RKGTKEARL PSQVVWIRRL RVLRRLLAKY RDAGKIDRHL YHSLYKSAKG NTFKHKRALV EHIIQAKADA QREKALKEEA E ARRSKNRA ARERRAQRVA EKREALLKED A

UniProtKB: KLLA0E12453p

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Macromolecule #21: 60S ribosomal protein L20

MacromoleculeName: 60S ribosomal protein L20 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 20.494939 KDa
SequenceString:
MNLRKEYQVI GRRLPTESVP EPKLFRMRIF APNDVVAKSR YWYFLQKLHK VKKASGEIVS LNVIHEAHPT TVKNFGVWVR YDSRSGTHN MYKEIRDVTR VGAVESLYQD MAARHRARFR SIHILKVVEL EKTADVKRQY VKQFLTKDLK FPLPHRVQKT N KVYAYKRP STFY

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Macromolecule #22: KLLA0E23651p

MacromoleculeName: KLLA0E23651p / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 18.214252 KDa
SequenceString:
MGKSHGYRSR TRYMFQRDFR KHGAIALSTY MKVYKVGDIV DIKANGSIHK GMPHKFYQGK TGVVYNVTKS SVGIIINKMV GNRYLEKRL NLRVEHIKHS KCRQEFLERV KSNAAKKAEA KAQGVAVQLK RQPAQPKESR VVSTEGNVPQ TLAPVPYETF I

UniProtKB: KLLA0E23651p

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Macromolecule #23: KLLA0D05181p

MacromoleculeName: KLLA0D05181p / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.993671 KDa
SequenceString:
MAPNTARKQK ITKTFTVDVS SPTENGVFDP ASYAKYLIDH IKVEGHVGNL GQAITVEEDG SVVTIVSTTK FSGKYLKYLT KKYLKKNQL RDWIRFVSTK TNEYKLAFYQ ITPEDEEEEE DEE

UniProtKB: KLLA0D05181p

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Macromolecule #24: KLLA0E06997p

MacromoleculeName: KLLA0E06997p / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.485948 KDa
SequenceString:
MSGNGAQGTK FRISLGLPTG AIMNCADNSG ARNLYIMAVK GSGSRLNRLP AASLGDMVMA TVKKGKPELR KKVMPAIVVR QSKAWRRKD GVFLYFEDNA GVIANPKGEM KGSAVTGPVG KECADLWPRI ASNSGVVV

UniProtKB: KLLA0E06997p

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Macromolecule #25: 60S ribosomal protein L24

MacromoleculeName: 60S ribosomal protein L24 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 17.638668 KDa
SequenceString:
MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQSSKSASLF HQRKNPRRIA WTVLYRRHHK KGITEEVARK RTRKSVKAQR AVVGASLEL IKERRSLKPE VRKAQRDEKK KADKEKKKAD KAARKSEKAK LAAAQGSKVS KQQAKGAFQK VAATSR

UniProtKB: Large ribosomal subunit protein eL24

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Macromolecule #26: 60S ribosomal protein L25

MacromoleculeName: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.746497 KDa
SequenceString:
MAPSTKATAA KKAVVKGTNG KKALKVRTSA SFRLPKTLKL ARSPKYATKA VPHYNRLDSY KVIEQPITSE TAMKKVEDGN TLVFKVSLK ANKYQIKKAV KELYEVDVLS VNTLVRPNGT KKAYVRLTAD FDALDIANRI GYI

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #27: KLLA0B05742p

MacromoleculeName: KLLA0B05742p / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.329735 KDa
SequenceString:
MAKQSLDVSS DRRKARKAYF TAPSSERRVL LSAPLSKELR EQYNIKALPI RKEDEVLVVR GSKKGQEGKV SSVYRLKFAV QVDKLTKEK SNGASVPTNI HPSKVVITKL HLDKDRKALI QRKGGKLE

UniProtKB: KLLA0B05742p

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Macromolecule #28: KLLA0E03455p

MacromoleculeName: KLLA0E03455p / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.600354 KDa
SequenceString:
MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGTKSHPFGH ALVAGIERYP LKVTKRQGAK KVAKRTKIKP FIKVINYNHL LPTRYTLDV EAFKSVVSTE TFEEPSQREE AKKVIKKAFE ERHQAGKNQW FFTKLNF

UniProtKB: KLLA0E03455p

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Macromolecule #29: RPL28

MacromoleculeName: RPL28 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 16.584479 KDa
SequenceString:
MPTRLTKTRK HRGHVSAGKG RVGKHRKHPG GRGMAGGLHH HRTNLDKYHP GYFGKVGMRY FHKQQAHFWK PVLNLDKLWT LVPEEKREE YLKSASKSAA PVIDTLAAGY GKVLGKGRIP DVPVIVKARF VSKLAEEKIK AAGGVVELIA

UniProtKB: RPL28

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Macromolecule #30: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 7.09036 KDa
SequenceString:
MAKSKNHTAH NQTKKAHRNG IKKPKTYKYP SLKGVDAKFR RNHRFALHGT AKALAAKRAE KK

UniProtKB: 60S ribosomal protein L29

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Macromolecule #31: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 11.382281 KDa
SequenceString:
MAPAKNQENI NQKLALVVKS GKYSLGYKST VKSLRQGKAK LIIIAANTPV LRKSELEYYA MLSKTKVYYF QGGNNELGTA VGKLFRVGV VTILDAGDSD ILTALA

UniProtKB: Large ribosomal subunit protein eL30

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Macromolecule #32: KLLA0B02937p

MacromoleculeName: KLLA0B02937p / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.177415 KDa
SequenceString:
MAGLKDVVTR EYTINMHKRL HGVSFKKRAP KAVKEIKKFA KLHMGTDDVR LDPKLNQEIW KRGIKGVPFR LRLRISRRRN EEEDAKNPL FSYVEPVVVA SAKGLHTVVV DEDEA

UniProtKB: KLLA0B02937p

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Macromolecule #33: KLLA0E06843p

MacromoleculeName: KLLA0E06843p / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.686289 KDa
SequenceString:
MAASLHPKIV KKHTKKFKRH HSDRYHRVSE NWRKQKGIDS VVRRRFRGNI SEPTIGYGSN KKTKFMGPDG RKVVLVSNVK GLTPLLMHT KTFAAEIAHN VSSKNRIAIL AEAKKNGIKV TNAKARLALE A

UniProtKB: KLLA0E06843p

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Macromolecule #34: KLLA0D07405p

MacromoleculeName: KLLA0D07405p / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 12.108021 KDa
SequenceString:
MAESHRLYVK GKHLSYQRSK SVNNPNVSLV KIEGVANPED AKFYLGKRVA YVYRCSKEVR GSKIRVIWGK INRTHGNSGV VRATFRSNL PAKTFGASVR IFLYPSNI

UniProtKB: KLLA0D07405p

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Macromolecule #35: KLLA0C08371p

MacromoleculeName: KLLA0C08371p / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.056568 KDa
SequenceString:
MAQRVTFRRR NPYNTKSNKI KVVKTPGGAL RSQHVKKLAT RPKCGDTGVP LQGVSTLRPR QYATVSRTKK TVSRAYGGSK SANAVKERI VRAFLIEEQK IVKRVIKEQT EAAKKAEKKD AKKSKK

UniProtKB: KLLA0C08371p

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Macromolecule #36: KLLA0F05247p

MacromoleculeName: KLLA0F05247p / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.079717 KDa
SequenceString:
MAGIKAYELR TKSKDQLEQQ LVELKKELAE LKVQKLSRPS LPKINTVRKN IARVLTVISQ NQRQAVRELY KGKKYQPKDL RAKKTRALR RALTKFEASQ VTEKQRKKQI AFPQRKYAIK A

UniProtKB: KLLA0F05247p

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Macromolecule #37: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 11.128179 KDa
SequenceString:
MAVKSGIAVG LNKGKKVNQL TPAPKISYRK GAASQRTTFV RSIVKEVASL APYERRLIEL IRNAGEKRAR KVAKKRLGTF GRAKAKVEE MNEIITASRR H

UniProtKB: 60S ribosomal protein L36

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Macromolecule #38: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 9.968549 KDa
SequenceString:
MGKGTPSFGK RHNKSHTLCK RCGRSSYHIQ KHTCSSCGYP AAKMRSHNWA AKAKRRRTTG TGRMRYLKHV SRRFKNGFQS GEAKAQSA

UniProtKB: Ribosomal protein L37

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Macromolecule #39: KLLA0C18216p

MacromoleculeName: KLLA0C18216p / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 8.996661 KDa
SequenceString:
MAKEIADIKE FLELIRRQDV NSATVKINKK LNKNGKAFRQ TKFKLRGSRY LYTLIVNDAG KAKKLLQSLP PTFNVNKL

UniProtKB: KLLA0C18216p

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Macromolecule #40: 60S ribosomal protein L39

MacromoleculeName: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 6.35864 KDa
SequenceString:
MAAQKSFRIK QKMAKAKKQN RPLPQWIRLR TNNTIRYNAK RRNWRRTKMN I

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Macromolecule #41: Ubiquitin fusion protein

MacromoleculeName: Ubiquitin fusion protein / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.597104 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLKALASKY NCEKSVCRKC YARLPPRATN CRKRKCGHTN QLRPKKKLK

UniProtKB: Ubiquitin fusion protein

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Macromolecule #42: 60S ribosomal protein L41

MacromoleculeName: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 3.137985 KDa
SequenceString:
MRAKWRKKRT RRLKRKRRKV RAR

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Macromolecule #43: 60S ribosomal protein L44

MacromoleculeName: 60S ribosomal protein L44 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 12.267632 KDa
SequenceString:
MVNVPKTRKT YCKGKECRKH AQHKVTQYKA GKASLYAQGK RRYDRKQSGF GGQTKQIFHK KAKTTKKVVL RLECMSCKTK TQLALKRCK HFELGGEKKQ KGQALQF

UniProtKB: Large ribosomal subunit protein eL42

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Macromolecule #44: KLLA0E05941p

MacromoleculeName: KLLA0E05941p / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 9.970605 KDa
SequenceString:
MAKRTKKVGI TGKYGVRYGS SLRRQVKKLE VQQHATYNCS FCGKTCVKRG AAGVWSCSSC NRTIAGGAYT LSTAAAATVR STIRRLRDM AEA

UniProtKB: KLLA0E05941p

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Macromolecule #45: Ribosomal protein

MacromoleculeName: Ribosomal protein / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 24.488783 KDa
SequenceString: MSKITSSHVR DNVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GTLKLPVCPR PNLSICIFGD AFDVDRAKSC GVDAMSVED LKKLNKNKKL IKKLAKKYSA FVASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYQKVN DVKSTIKFQL K KVLCLAVA ...String:
MSKITSSHVR DNVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GTLKLPVCPR PNLSICIFGD AFDVDRAKSC GVDAMSVED LKKLNKNKKL IKKLAKKYSA FVASEVLIKQ VPRLLGPQLS KAGKFPTPVS HNDDLYQKVN DVKSTIKFQL K KVLCLAVA VGNVEMDEDT LVNQILMSVN FLVSLLKKNW QNVGSLVIKS TMGPAYRLY

UniProtKB: Ribosomal protein

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Macromolecule #46: 60S acidic ribosomal protein P0

MacromoleculeName: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 33.606969 KDa
SequenceString: MGGVREKKAE YFAKLREYLE EYKSVFVVGV DNVSSQQMHE VRKNLRGRAV VLMGKNTMVR RAVRGFISDF PDYEKLLPFV KGNVGFIFT NDSLQDIKEV IIANKVAAPA RAGAVAPEDI WVLAVNTGME PGKTSFFQAL GVPTKIARGT IEIVSDVKVV D AGKRVGAS ...String:
MGGVREKKAE YFAKLREYLE EYKSVFVVGV DNVSSQQMHE VRKNLRGRAV VLMGKNTMVR RAVRGFISDF PDYEKLLPFV KGNVGFIFT NDSLQDIKEV IIANKVAAPA RAGAVAPEDI WVLAVNTGME PGKTSFFQAL GVPTKIARGT IEIVSDVKVV D AGKRVGAS EASLLNLLNI SPFTYGLTVV QVYDNGQVFP ASILDITDDE LISHFISAVN TIACVSLAAG YPTLPSVGHS LI NNYKDLL AVAIAADYIY PEIEELKDRI ENPDAYASAA PVAAASGSSE AAAEEAPAEE EEESDDDMGF GLFD

UniProtKB: 60S acidic ribosomal protein P0

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Macromolecule #47: GDPCP

MacromoleculeName: GDPCP / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 12.528436 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)

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Macromolecule #48: 40S ribosomal protein S0

MacromoleculeName: 40S ribosomal protein S0 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 28.264525 KDa
SequenceString: MSLPSTFDLT SEDAQLLLAA RVHLGAKNVQ VHQEPYVYKA RPDGVNVINV GKTWEKIVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKY AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKESSYV NIPVIALTDL DSPSEYVDVA I PCNNRGKH ...String:
MSLPSTFDLT SEDAQLLLAA RVHLGAKNVQ VHQEPYVYKA RPDGVNVINV GKTWEKIVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKY AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKESSYV NIPVIALTDL DSPSEYVDVA I PCNNRGKH SIGLIWYLLA REVLRLRGAL PDRTQPWAIM PDLYFYRNPE EIEQQTAEEE AVASGEQTEE AVDATEEQTE AA EWAEEGQ AQEEEWN

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #49: 40S ribosomal protein S1

MacromoleculeName: 40S ribosomal protein S1 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 28.971643 KDa
SequenceString: MAVGKNKRLS KGKKGLKKRV VDPFTRKEWY DIKAPSTFEN RNVGKTLVNK SVGLKNASDS LKGRVVEVCL ADLQGSEDHS FRKVKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRTSYAQSS H IRQIRKVI ...String:
MAVGKNKRLS KGKKGLKKRV VDPFTRKEWY DIKAPSTFEN RNVGKTLVNK SVGLKNASDS LKGRVVEVCL ADLQGSEDHS FRKVKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRTSYAQSS H IRQIRKVI SEILTREVQN STLAQLTSKL IPEVINKEIE NATKDIFPLQ NVHIRKVKLL KQPKFDLGSL LSLHGEASAE EK GKKVAGF KDEILETV

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #50: KLLA0F09812p

MacromoleculeName: KLLA0F09812p / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 27.649979 KDa
SequenceString: MSAPQAQGQQ APRRGGFGGA NRGGRGGRRG GRRDQEEKGW VPVTKLGRLV KAGKISSIEE IFLHSLPVKE FQIIDQLLPN LKDEVMNIK PVQKQTRAGQ RTRFKAVVVV GDSNGHVGLG IKTAKEVAGA IRAGIIIAKL SVIPIRRGYW GTNLGQPHSL A TKTSGKCG ...String:
MSAPQAQGQQ APRRGGFGGA NRGGRGGRRG GRRDQEEKGW VPVTKLGRLV KAGKISSIEE IFLHSLPVKE FQIIDQLLPN LKDEVMNIK PVQKQTRAGQ RTRFKAVVVV GDSNGHVGLG IKTAKEVAGA IRAGIIIAKL SVIPIRRGYW GTNLGQPHSL A TKTSGKCG SVSVRLIPAP RGSGIVASPA VKKLMQLAGV EDVYTSSTGS TRTLENTLKA AFVAIGNTYG FLTPNLWEVQ AL TPSPMDV YADYATASKK KL

UniProtKB: KLLA0F09812p

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Macromolecule #51: KLLA0D08305p

MacromoleculeName: KLLA0D08305p / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 26.300535 KDa
SequenceString: MVAIISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEIII RATKVQDVVG ENGRRINELT LLIEKRFKYK RGTIALYAE RVHDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVIS GKLRAARAKS MKFADGFLIH S GQPVNDFI ...String:
MVAIISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEIII RATKVQDVVG ENGRRINELT LLIEKRFKYK RGTIALYAE RVHDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVIS GKLRAARAKS MKFADGFLIH S GQPVNDFI ETATRHVLLR QGVLGIKVKI MKDPSRNTSG PKALPDAVTI IEPKEEEPVL EPSVKDYRPT EPVEAAESA

UniProtKB: 40S ribosomal protein S3

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Macromolecule #52: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 29.617514 KDa
SequenceString: MARGPKKHLK RLAAPHHWML DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TFPAGFMDV ITLEATNENF RLVYDVKGRF AVHRITDEEA SYKLAKVKKV QLGKKGIPYV VTHDGRTIRY PDPNIKVNDT V KVDLATGT ...String:
MARGPKKHLK RLAAPHHWML DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TFPAGFMDV ITLEATNENF RLVYDVKGRF AVHRITDEEA SYKLAKVKKV QLGKKGIPYV VTHDGRTIRY PDPNIKVNDT V KVDLATGT ITDFIKFDTG KLVYVTGGRN LGRVGTIVHR ERHEGGFDLV HIKDSLENTF VTRLNNVFVI GEPGRPWISL PK GKGIKLT ISEERDRRRA QHGL

UniProtKB: 40S ribosomal protein S4

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Macromolecule #53: KLLA0D10659p

MacromoleculeName: KLLA0D10659p / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 25.385975 KDa
SequenceString: MSEHEAQVEV EVQEDFEVVQ EFVPVELATT IPVEIQQAQQ EIKLFNKWSF EDVEVKDASL VDYIQISKPI YVAHTAGRYA NKRFRKAQC PIVERLTNSL MMNGRNNGKK LKAVRIVKHT LEIINVLTDQ NPLQVVVDAI INSGPREDTT RVGGGGAARR Q AVDVSPLR ...String:
MSEHEAQVEV EVQEDFEVVQ EFVPVELATT IPVEIQQAQQ EIKLFNKWSF EDVEVKDASL VDYIQISKPI YVAHTAGRYA NKRFRKAQC PIVERLTNSL MMNGRNNGKK LKAVRIVKHT LEIINVLTDQ NPLQVVVDAI INSGPREDTT RVGGGGAARR Q AVDVSPLR RVNQSIALLT IGAREAAFRN IKTIAETLAE ELINAAKGSS TSYAIKKKDE LERVAKSNR

UniProtKB: KLLA0D10659p

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Macromolecule #54: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 26.970391 KDa
SequenceString: MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS ...String:
MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS YTKAPKIQRL VTPQRLQRKR QQKSLKIKNA QAQREAAAEY AQLLAKRLSE RKAEKAEVRK RRASSLKA

UniProtKB: Small ribosomal subunit protein eS6

+
Macromolecule #55: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 21.735297 KDa
SequenceString:
MSDPQAKILS QAPTELELQV AQAFIDLENN SPELKADLRA LQFKSIREIE VAGGKKALAV FVPVPSLAAY HKVQIKLTRE LEKKFQDRH VIFLAERRIL PKPSRKSRQT QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKILLNSKDV Q HIDNKLES FQAVYNKLTG KQIVFEIPSE TH

UniProtKB: 40S ribosomal protein S7

+
Macromolecule #56: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 22.642727 KDa
SequenceString: MGISRDSRHK RAATGAKRAQ FRKKRKFELG RQAANTKIGT KRIHPVRTRG GNQKFRALRI ETGNFSWASE GVARKTRITG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWYESHYGQ SLGKKKNTKA EEETATTSKN TERKWAARAA EAKIEHAVDS Q FGAGRLYA ...String:
MGISRDSRHK RAATGAKRAQ FRKKRKFELG RQAANTKIGT KRIHPVRTRG GNQKFRALRI ETGNFSWASE GVARKTRITG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWYESHYGQ SLGKKKNTKA EEETATTSKN TERKWAARAA EAKIEHAVDS Q FGAGRLYA AISSRPGQSG RCDGYILEGE ELAFYLRRLT AKK

UniProtKB: 40S ribosomal protein S8

+
Macromolecule #57: KLLA0E23673p

MacromoleculeName: KLLA0E23673p / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 21.587049 KDa
SequenceString:
MPRAPRTYSK TYSTPKRPYE SARLDAELKL AGEYGLKNKR EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRIGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI SQRHIAVGKQ IVNIPSFMVR LESEKHIDFA R TSPFGGAR PGRVARKRAA AAGGEEADEE

UniProtKB: KLLA0E23673p

+
Macromolecule #58: KLLA0B08173p

MacromoleculeName: KLLA0B08173p / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 12.584377 KDa
SequenceString:
MLIPKEDRKK IYQHLFQEGV LVAKKDFNQP KHEEIDTKNL FVIKALQSLT SKGFVKTQFS WQYYYYTLTE EGVVYLREYL NLPEHIFPA TYLAGQSGDQ RPQGKKY

UniProtKB: KLLA0B08173p

+
Macromolecule #59: KLLA0A10483p

MacromoleculeName: KLLA0A10483p / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 17.84393 KDa
SequenceString:
MSTELTVQSE RAFQKQPHIF TNPKAKANRK TKRWYKNVGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTRM HRTIVIRRD YLHYVPKYNR YEKRHKNVPA HVSPAFRVQV GDIVTVGQCR PISKTVRFNV LKVASATGKA NKQFAKF

UniProtKB: KLLA0A10483p

+
Macromolecule #60: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.466398 KDa
SequenceString:
MSDVEEVQQV PVAELTIEDA LKVVLRTSLV HDGLARGLRE SAKALTRGEG QLAVLVESVT EEAISKLVQG LATENNVPLI KVADAKQLG EWAGLGKIDR DGNARKVVGA SVVVVKNWGA DTQEREILLE HFSQQ

UniProtKB: 40S ribosomal protein S12

+
Macromolecule #61: KLLA0F18040p

MacromoleculeName: KLLA0F18040p / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 16.989875 KDa
SequenceString:
MGRMHSKGKG MSSSAIPYSR NAPAWFKGSS DGVVEQIIKY ARKGLTPSQI GVLLRDAHGV TQAKVITGNK ILRILKSNGL APEIPEDLY FLIKKAVSVR KHLERNRKDK DAKFRLILIE SRIHRLARYY RTVSVLPPNW KYESATASAL VN

UniProtKB: KLLA0F18040p

+
Macromolecule #62: 40S ribosomal protein S14

MacromoleculeName: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.530655 KDa
SequenceString:
MANVVQAKDN SQVFGVARIF ASFNDTFVHV TDLSGRETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH IKIRATGGT RSKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11

+
Macromolecule #63: KLLA0F07843p

MacromoleculeName: KLLA0F07843p / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.986796 KDa
SequenceString:
MSEAAAPRKR SFKTYSYKGV DLEKLLEMPT EDFVKLAPAR VRRKFARGLS EKPAGLMKKL RAAKLSAPEN EKPAVVRTHL RNMIIVPEM IGSVVGVYNG KVFNQVEIRP EMVGHYLGEF SITYTPVRHG RAGATTSRFI PLR

UniProtKB: KLLA0F07843p

+
Macromolecule #64: 40S ribosomal protein S16

MacromoleculeName: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.874531 KDa
SequenceString:
MSTVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVQ PEILRFKVYE PLLLVGLDKF ANIDIRVKVT GGGHVSQVYA IRQAIAKGL VAYHQKFVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGRGARSRFQ KSYR

UniProtKB: Small ribosomal subunit protein uS9

+
Macromolecule #65: KLLA0B01474p

MacromoleculeName: KLLA0B01474p / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.722216 KDa
SequenceString:
MGRVRTKTVK RASKALIEKY YPKLTMDFQT NKRLCDEIAT IQSKRLRNKI AGYTTHLMKR IQKGPVRGIS FKLQEEERER KDQYVPDVS ALDLSHSNDV LNVDTQTAEL VNSLGLKLPL SVSSVSAVRD RRFRKRN

UniProtKB: KLLA0B01474p

+
Macromolecule #66: KLLA0B01562p

MacromoleculeName: KLLA0B01562p / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 17.084602 KDa
SequenceString:
MSLVVQEQGS FQHILRLLNT NVDGNINVVY ALTTIRGVGR RYANLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ KDVNDGKDYH SLANNLESKL RDDLERLKKI RSHRGIRHFW GLRVRGQHTK TTGRRRA

UniProtKB: KLLA0B01562p

+
Macromolecule #67: KLLA0A07194p

MacromoleculeName: KLLA0A07194p / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.87901 KDa
SequenceString:
MPGVSVRDVP AQDFINNYAS FLQRQGKLEV PGYVDIVKTS AGNELPPQDS EGWFYKRAAS VARHIYLRKQ VGVGKLNKLY GGAKNRGVR PHKHVDASGS INRKVLQSLE KLGVVEISPK GGRRISDNGL RDLDRIAAAT LEDEE

UniProtKB: KLLA0A07194p

+
Macromolecule #68: KLLA0F25542p

MacromoleculeName: KLLA0F25542p / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.337604 KDa
SequenceString:
MSQVEKKSEQ QQEVVIHKIR INLTSTKVKQ LENVSANIIK NAETFKLVKK GPVRLPTKVL KISTRKTPNG EGSKTWDTYE MRIHKRYID LEAPAHIVKR ITQITIEPGV DVEVIIAA

UniProtKB: KLLA0F25542p

+
Macromolecule #69: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 9.797949 KDa
SequenceString:
MENDKGQLVE LYVPRKCSAT NRIIKAKDHS SVQINIAQVD EEGRAIPGEY VTYALSGYIR ARGEADDSLN RLAQQDGLLK NVWSYSR

UniProtKB: Small ribosomal subunit protein eS21

+
Macromolecule #70: 40S ribosomal protein S22

MacromoleculeName: 40S ribosomal protein S22 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 14.645041 KDa
SequenceString:
MTRTSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK IVVQLNGRLN KCGVISPRFN VKIADVEKW TANLLPARQF GYVILTTSAG IMDHEEAHRK HVSGKILGFV Y

UniProtKB: Small ribosomal subunit protein uS8

+
Macromolecule #71: RPS23

MacromoleculeName: RPS23 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 16.047897 KDa
SequenceString:
MGKGKPRGLN SARKLRVHRR NNRWAETTYK KRLLGTAFKS SPFGGSSHAK GIVLEKIGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

UniProtKB: RPS23

+
Macromolecule #72: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 15.194549 KDa
SequenceString:
MSDAITIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEAYKA EKDAVSVFGF RTQYGGGKST GFGLVYNSVA DAKKFEPAY RLVRYGLAEK VEKASRQQRK QRKNRGKKIF GTGKSIAKKA ARRNAD

UniProtKB: 40S ribosomal protein S24

+
Macromolecule #73: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 12.002116 KDa
SequenceString:
MPPKQQLSKA AKAAAAMAGG KKSKKKWSKK SHKDKAKHAV VLDQDKFDRI MKEAPTYRYV SVSVLVDRFK LGGSLARVAL RHLENEGII KPVSKHSKQA IYTRATASE

UniProtKB: 40S ribosomal protein S25

+
Macromolecule #74: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 13.539957 KDa
SequenceString:
MPKKRASNGR NKKGRGHVKP VRCVNCSRSV PKDKAIKRMA IRNIVEAAAI RDLSEASVYA EYALPKTYNK LHYCISCAIH ARIVRVRSR TDRRIRAPPQ RPRFNRDNKV SPADAAKKAL

UniProtKB: 40S ribosomal protein S26

+
Macromolecule #75: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 8.884362 KDa
SequenceString:
MVLVQDLLHP TAASEARKHK LKTLVQSPRS HFLDVKCPGC LNITTVFSHA QTAVTCESCS TVLCTPTGGK AKLSEGTSFR RK

UniProtKB: 40S ribosomal protein S27

+
Macromolecule #76: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 7.549824 KDa
SequenceString:
MDTKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTTRTI VRNVKGPVRE GDILVLMESE REARRLR

UniProtKB: Small ribosomal subunit protein eS28

+
Macromolecule #77: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 6.66257 KDa
SequenceString:
MAHENVWYSH PRKFGKGSRQ CRISGSHSGL IRKYGLNIDR QSFREKANDI GFYKYR

UniProtKB: Small ribosomal subunit protein uS14

+
Macromolecule #78: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 7.141421 KDa
SequenceString:
MGKVHGSLAR AGKVKSQTPK VEKQEKPKQP KGRAYKRLLY TRRFVNVTLT NGKRKMNPSP SSQ

UniProtKB: 40S ribosomal protein S30

+
Macromolecule #79: Ubiquitin-40S ribosomal protein S27a

MacromoleculeName: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 17.110977 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGGKKR KKKVYTTPK KIRHKHKKVK LAVLNYYKVD DEGKVAKLRK ECPNCGPGIF LANHGDRFYC GKCHSTFATQ K

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

+
Macromolecule #80: KLLA0E12277p

MacromoleculeName: KLLA0E12277p / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 35.830945 KDa
SequenceString: MSSSNIMLVL RGTLEGHNGW VTSLSTSAAQ PNLLVSGSRD KTLISWRLTE NEQQFGVPVR SYKGHSHIVQ DVVVSADGNY AVSASWDKT LRLWNLATGN SEARFVGHTG DVLSVAIDAN SSKIISASRD KTIRVWNTVG DCAYVLLGHT DWVTKVRVAP K NLEDGEVD ...String:
MSSSNIMLVL RGTLEGHNGW VTSLSTSAAQ PNLLVSGSRD KTLISWRLTE NEQQFGVPVR SYKGHSHIVQ DVVVSADGNY AVSASWDKT LRLWNLATGN SEARFVGHTG DVLSVAIDAN SSKIISASRD KTIRVWNTVG DCAYVLLGHT DWVTKVRVAP K NLEDGEVD DGRITFVSAG MDKIVRSWSL NEDSYRIEAD FIGHNNYINV VQPSPDGSLA ASAGKDGQIY VWNLKHKSAF MN FDAKDEV FALAFSPSRF WLTAATASGI KIYDLENEVL IDELKPEFAG YTKAQDPHAV SLAWSADGQT LFAGYTDNVI RVW QVMTAN

UniProtKB: KLLA0E12277p

+
Macromolecule #82: KLLA0F23265p

MacromoleculeName: KLLA0F23265p / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis (yeast)
Molecular weightTheoretical: 108.918234 KDa
SequenceString: MAKKSKKNQQ NYWDEEYEED VPQESSVEPS IDGSVEPSGE TEVADASAIE EDFMSTLKKS KKKLDKKEDE DTKKPVLKSK KEKEKEKKE KEKEKKREQA AKKKAQQQAQ KENNKKLHAE QTSKLKAEKE AGKSTEGAAP KAAPKKKVPA GLAALKRQLE L QKQLEEEE ...String:
MAKKSKKNQQ NYWDEEYEED VPQESSVEPS IDGSVEPSGE TEVADASAIE EDFMSTLKKS KKKLDKKEDE DTKKPVLKSK KEKEKEKKE KEKEKKREQA AKKKAQQQAQ KENNKKLHAE QTSKLKAEKE AGKSTEGAAP KAAPKKKVPA GLAALKRQLE L QKQLEEEE RLAREEEERL EKEEEEKLAK AEQEKEEARK AKREREQAKR EKLKAEGKLL TKKQKEEKKL LENRRAALLA AG NIKVAGL EKSGDEHPKP KKVVYGKRVR KTKEQIESEK AIEEEKKKAT ETQEVEEEEE ALIDDWENLA LDDEPEEQEG KEE TSEKEQ YSGSETSNAT TPAPELSTEV TKNYASQPIP SKRSAAAAKK DLRSPICCIL GHVDTGKTKL LDKIRQTNVQ GGEA GGITQ QIGATYFPID AIEQKTAVMA KYEEQTFDVP GLLVIDTPGH ESFTNLRSRG SSLCNIAILV IDIMHGLEQQ TIESI RLLR DRKAPFIVAL NKIDRLYDWV ITPNNSFRDS FDKQERAVKQ EFQTRLDNIK VALAEQGLNS ELYFQNNNLA KYVSIV PTS AVTGEGVPDL LWLLLELTQK RMSKQLKYLS HVEATILEVK VVEGFGTTID VILSNGYLRE GDRIVLCGMN GPIVTNI RA LLTPQPLREL RLKSEYQHHK EVKAALGVKI AANDLEKAVS GSRLLVVGPD DDEEEMMDDV MEDLTGLLDS VDTSGRGV V VQASTLGSLE ALLDFLKDMK IPVMSVGLGP VYKRDIMKAC TMLEKAPELA VMLCFDVKVD KEAEQYAAEQ HIKIFNADI IYHLFDAFTA YQKELLEKRR AEFIGDATFP CVLNTLQIIN KRGPMIIGVD VIEGSLKIGT PICAVKYDPA TKERITLLLG RVTSLEINH QSVTEVKKGQ TNAGVAMRLE DPSGQQPIWG RHVDEKDTLY SHITRRSIDT LKDPAFRDQV PKSDWMLIRK L KPVFGIE

UniProtKB: KLLA0F23265p

+
Macromolecule #84: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 84 / Number of copies: 6 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #85: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

MacromoleculeName: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 85 / Number of copies: 1 / Formula: GCP
Molecular weightTheoretical: 521.208 Da
Chemical component information

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 6
GridModel: Quantifoil R2/2 / Material: GOLD / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 3 / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K

-
Electron microscopy

MicroscopeFEI POLARA 300
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 64815
Startup modelType of model: EMDB MAP
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 29712
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

-
Atomic model buiding 1

Initial modelPDB ID:

3u5b
PDB Unreleased entry


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementOverall B value: 110.6
Output model

PDB-6uz7:
K.lactis 80S ribosome with p/PE tRNA and eIF5B

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