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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-0868 | |||||||||
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Title | Cryo-EM structure of the AtMLKL2 tetramer | |||||||||
![]() | None | |||||||||
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![]() | Plant MLKLs / Cryo-EM / Tetramer / CYTOSOLIC PROTEIN | |||||||||
Function / homology | ![]() protein serine/threonine/tyrosine kinase activity / protein kinase activity / signal transduction / ATP binding / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.1 Å | |||||||||
![]() | Lisa M / Huang M | |||||||||
![]() | ![]() Title: Cryo-EM structure of the AtMLKL3 tetramer Authors: Lisa M / Huang M / Zhang X / Ryohei TN / Leila BK / Isabel MLS / Florence J / Viera K / Dmitry L / Jane EP / James MM / Kay H / Paul SL / Chai J / Takaki M | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 3.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.1 KB 9.1 KB | Display Display | ![]() |
Images | ![]() | 79 KB | ||
Filedesc metadata | ![]() | 5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 389.9 KB | Display | ![]() |
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Full document | ![]() | 389.4 KB | Display | |
Data in XML | ![]() | 5.7 KB | Display | |
Data in CIF | ![]() | 6.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6lbaMC ![]() 9954C ![]() 6ka4C C: citing same article ( M: atomic model generated by this map |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | None | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.30654 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Mixed lineage kinase domain-like (MLKL) protein 2
Entire | Name: Mixed lineage kinase domain-like (MLKL) protein 2 |
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Components |
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-Supramolecule #1: Mixed lineage kinase domain-like (MLKL) protein 2
Supramolecule | Name: Mixed lineage kinase domain-like (MLKL) protein 2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Protein kinase family protein
Macromolecule | Name: Protein kinase family protein / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 66.682531 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: AAMEQFRQIG EVLGSLNALM VLQDDILINQ RQCCLLLELF SLAFNTVAEE IRQNLKLEEK HTKWRALEQP LRELTRVFKE GELYVKHCM DNSDWWGKVI NLHQNKDCVE FHIHNLFCYF SAVVEAIEAA GEISGLDPSE MERRRVVFSR KYDREWNDPK M FQWRFGKQ ...String: AAMEQFRQIG EVLGSLNALM VLQDDILINQ RQCCLLLELF SLAFNTVAEE IRQNLKLEEK HTKWRALEQP LRELTRVFKE GELYVKHCM DNSDWWGKVI NLHQNKDCVE FHIHNLFCYF SAVVEAIEAA GEISGLDPSE MERRRVVFSR KYDREWNDPK M FQWRFGKQ YLLSRDICSR FEHSWREDRW NLVEALQEKR KSDSDDIGKT EKRLADLLLK KLTGLEQFNG KLFPSSILLG SK DYQVKKR LDADGQYKEI QWLGDSFALR HFFSDLEPLS SEISSLLALC HSNILQYLCG FYDEERKECF LVMELMHKDL QSY MKENCG PRRRYLFSIP VVIDIMLQIA RGMEYLHGND IFHGDLNPMN IHLKERSHTE GYFHAKICGF GLSSVVKAQS SSKP GTPDP VIWYAPEVLA EMEQDLNGKT PKSKLTHKAD VYSFAMVCFE LITGKVPFED SHLQGEPMTI NIRMGERPLF PFPSP KTLV SLIKRCWHSE PSQRPNFSSI CRILRYIKKF LVVNPDHGHP QMQTPLVDCW DLEARFLRKF PGDAGSHTAS VNQIPF QLT SYRVLEKEK UniProtKB: Protein kinase family protein |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | 3D array |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 1.5625 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 1527009 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |