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Open data
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Basic information
Entry | Database: PDB / ID: 6lba | ||||||
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Title | Cryo-EM structure of the AtMLKL2 tetramer | ||||||
![]() | Protein kinase family protein | ||||||
![]() | CYTOSOLIC PROTEIN / Plant MLKLs / Cryo-EM / Tetramer | ||||||
Function / homology | ![]() protein serine/threonine/tyrosine kinase activity / protein kinase activity / signal transduction / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.1 Å | ||||||
![]() | Lisa, M. / Huang, M. / Zhang, X. / Ryohei, T.N. / Leila, B.K. / Isabel, M.L.S. / Florence, J. / Viera, K. / Dmitry, L. / Jane, E.P. ...Lisa, M. / Huang, M. / Zhang, X. / Ryohei, T.N. / Leila, B.K. / Isabel, M.L.S. / Florence, J. / Viera, K. / Dmitry, L. / Jane, E.P. / James, M.M. / Kay, H. / Paul, S.L. / Chai, J. / Takaki, M. | ||||||
![]() | ![]() Title: Cryo-EM structure of the AtMLKL3 tetramer Authors: Lisa, M. / Huang, M. / Zhang, X. / Ryohei, T.N. / Leila, B.K. / Isabel, M.L.S. / Florence, J. / Viera, K. / Dmitry, L. / Jane, E.P. / James, M.M. / Kay, H. / Paul, S.L. / Chai, J. / Takaki, M. | ||||||
History |
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Structure visualization
Movie |
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Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 336.1 KB | Display | ![]() |
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PDB format | ![]() | 272.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 824.8 KB | Display | ![]() |
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Full document | ![]() | 979.4 KB | Display | |
Data in XML | ![]() | 78 KB | Display | |
Data in CIF | ![]() | 109.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 0868MC ![]() 9954C ![]() 6ka4C C: citing same article ( M: map data used to model this data |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 66682.531 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: 3D ARRAY / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Mixed lineage kinase domain-like (MLKL) protein 2 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 1.5625 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
Software | Name: PHENIX / Version: 1.15.1_3469: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 1527009 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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