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4CVB
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CRYSTAL STRUCTURE OF QUINONE-DEPENDENT ALCOHOL DEHYDROGENASE FROM PSEUDOGLUCONOBACTER SACCHAROKETOGENENES
Descriptor:ALCOHOL DEHYDROGENASE, CALCIUM ION, PYRROLOQUINOLINE QUINONE, ...
Authors:Rozeboom, H.J., Yu, S., Mikkelsen, R., Nikolaev, I., Mulder, H., Dijkstra, B.W.
Deposit date:2014-03-25
Release date:2015-03-25
Last modified:2017-07-05
Method:X-RAY DIFFRACTION (1.72 Å)
Cite:Crystal Structure of Quinone-Dependent Alcohol Dehydrogenase from Pseudogluconobacter Saccharoketogenes. A Versatile Dehydrogenase Oxidizing Alcohols and Carbohydrates.
Protein Sci., 24, 2015
4CVC
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CRYSTAL STRUCTURE OF QUINONE-DEPENDENT ALCOHOL DEHYDROGENASE FROM PSEUDOGLUCONOBACTER SACCHAROKETOGENENES WITH ZINC IN THE ACTIVE SITE
Descriptor:ALCOHOL DEHYDROGENASE, DI(HYDROXYETHYL)ETHER, PYRROLOQUINOLINE QUINONE, ...
Authors:Rozeboom, H.J., Yu, S., Mikkelsen, R., Nikolaev, I., Mulder, H., Dijkstra, B.W.
Deposit date:2014-03-25
Release date:2015-03-25
Last modified:2016-01-27
Method:X-RAY DIFFRACTION (1.83 Å)
Cite:Crystal Structure of Quinone-Dependent Alcohol Dehydrogenase from Pseudogluconobacter Saccharoketogenes. A Versatile Dehydrogenase Oxidizing Alcohols and Carbohydrates.
Protein Sci., 24, 2015
2PYG
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AZOTOBACTER VINELANDII MANNURONAN C-5 EPIMERASE ALGE4 A-MODULE
Descriptor:Poly(beta-D-mannuronate) C5 epimerase 4, CALCIUM ION, GLYCEROL
Authors:Rozeboom, H.J., Bjerkan, T.M., Kalk, K.H., Ertesvag, H., Holtan, S., Aachmann, F.L., Valla, S., Dijkstra, B.W.
Deposit date:2007-05-16
Release date:2008-05-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Structural and Mutational Characterization of the Catalytic A-module of the Mannuronan C-5-epimerase AlgE4 from Azotobacter vinelandii.
J.Biol.Chem., 283, 2008
2PYH
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AZOTOBACTER VINELANDII MANNURONAN C-5 EPIMERASE ALGE4 A-MODULE COMPLEXED WITH MANNURONAN TRISACCHARIDE
Descriptor:Poly(beta-D-mannuronate) C5 epimerase 4, CALCIUM ION, alpha-D-mannopyranuronic acid
Authors:Rozeboom, H.J., Bjerkan, T.M., Kalk, K.H., Ertesvag, H., Holtan, S., Aachman, F.L., Valla, S., Dijkstra, B.W.
Deposit date:2007-05-16
Release date:2008-05-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.7 Å)
Cite:Structural and Mutational Characterization of the Catalytic A-module of the Mannuronan C-5-epimerase AlgE4 from Azotobacter vinelandii.
J.Biol.Chem., 283, 2008
2X2H
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CRYSTAL STRUCTURE OF THE GRACILARIOPSIS LEMANEIFORMIS ALPHA-1,4- GLUCAN LYASE
Descriptor:ALPHA-1,4-GLUCAN LYASE ISOZYME 1, GLYCEROL, ACETATE ION, ...
Authors:Rozeboom, H.J., Yu, S., Madrid, S., Kalk, K.H., Dijkstra, B.W.
Deposit date:2010-01-13
Release date:2011-01-19
Last modified:2019-02-06
Method:X-RAY DIFFRACTION (2.06 Å)
Cite:Crystal Structure of Alpha-1,4-Glucan Lyase, a Unique Glycoside Hydrolase Family Member with a Novel Catalytic Mechanism.
J.Biol.Chem., 288, 2013
2X2I
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CRYSTAL STRUCTURE OF THE GRACILARIOPSIS LEMANEIFORMIS ALPHA-1,4- GLUCAN LYASE WITH ACARBOSE
Descriptor:ALPHA-1,4-GLUCAN LYASE ISOZYME 1, BETA-ACARBOSE, GLYCEROL
Authors:Rozeboom, H.J., Yu, S., Madrid, S., Kalk, K.H., Dijkstra, B.W.
Deposit date:2010-01-13
Release date:2011-01-19
Last modified:2019-02-06
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Crystal Structure of Alpha-1,4-Glucan Lyase, a Unique Glycoside Hydrolase Family Member with a Novel Catalytic Mechanism.
J.Biol.Chem., 288, 2013
2X2J
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CRYSTAL STRUCTURE OF THE GRACILARIOPSIS LEMANEIFORMIS ALPHA- 1,4-GLUCAN LYASE WITH DEOXYNOJIRIMYCIN
Descriptor:ALPHA-1,4-GLUCAN LYASE ISOZYME 1, 1-DEOXYNOJIRIMYCIN, GLYCEROL, ...
Authors:Rozeboom, H.J., Yu, S., Madrid, S., Kalk, K.H., Dijkstra, B.W.
Deposit date:2010-01-13
Release date:2011-01-19
Last modified:2019-02-06
Method:X-RAY DIFFRACTION (2.35 Å)
Cite:Crystal Structure of Alpha-1,4-Glucan Lyase, a Unique Glycoside Hydrolase Family Member with a Novel Catalytic Mechanism.
J.Biol.Chem., 288, 2013
4AMW
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CRYSTAL STRUCTURE OF THE GRACILARIOPSIS LEMANEIFORMIS ALPHA-1,4- GLUCAN LYASE COVALENT INTERMEDIATE COMPLEX WITH 5-FLUORO-IDOSYL- FLUORIDE
Descriptor:ALPHA-1,4-GLUCAN LYASE ISOZYME 1, (2R,3R,4R,5S,6R)-6-fluoranyl-6-(hydroxymethyl)oxane-2,3,4,5-tetrol, GLYCEROL, ...
Authors:Rozeboom, H.J., Yu, S., Madrid, S., Kalk, K.H., Dijkstra, B.W.
Deposit date:2012-03-14
Release date:2013-03-27
Last modified:2019-02-06
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Crystal Structure of Alpha-1,4-Glucan Lyase, a Unique Glycoside Hydrolase Family Member with a Novel Catalytic Mechanism.
J.Biol.Chem., 288, 2013
4AMX
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CRYSTAL STRUCTURE OF THE GRACILARIOPSIS LEMANEIFORMIS ALPHA-1,4- GLUCAN LYASE COVALENT INTERMEDIATE COMPLEX WITH 5-FLUORO-GLUCOSYL- FLUORIDE
Descriptor:ALPHA-1,4-GLUCAN LYASE ISOZYME 1, 5-FLUORO-BETA-D-GLUCOPYRANOSE, GLYCEROL, ...
Authors:Rozeboom, H.J., Yu, S., Madrid, S., Kalk, K.H., Dijkstra, B.W.
Deposit date:2012-03-14
Release date:2013-03-27
Last modified:2019-02-06
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Crystal Structure of Alpha-1,4-Glucan Lyase, a Unique Glycoside Hydrolase Family Member with a Novel Catalytic Mechanism.
J.Biol.Chem., 288, 2013
4C2L
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CRYSTAL STRUCTURE OF ENDO-XYLOGALACTURONAN HYDROLASE FROM ASPERGILLUS TUBINGENSIS
Descriptor:ENDO-XYLOGALACTURONAN HYDROLASE A, ALPHA-D-MANNOSE, N-ACETYL-D-GLUCOSAMINE, ...
Authors:Rozeboom, H.J., Beldman, G., Schols, H.A., Dijkstra, B.W.
Deposit date:2013-08-19
Release date:2013-09-25
Last modified:2019-02-27
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Crystal Structure of Endo-Xylogalacturonan Hydrolase from Aspergillus Tubingensis.
FEBS J., 280, 2013
4CCW
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CRYSTAL STRUCTURE OF NAPROXEN ESTERASE (CARBOXYLESTERASE NP) FROM BACILLUS SUBTILIS
Descriptor:CARBOXYL ESTERASE NP, (2-hydroxyethoxy)acetic acid
Authors:Rozeboom, H.J., Godinho, L.F., Nardini, M., Quax, W.J., Dijkstra, B.W.
Deposit date:2013-10-29
Release date:2014-01-22
Last modified:2014-08-13
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Crystal Structures of Two Bacillus Carboxylesterases with Different Enantioselectivities.
Biochim.Biophys.Acta, 1844, 2014
4CCY
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CRYSTAL STRUCTURE OF CARBOXYLESTERASE CESB (YBFK) FROM BACILLUS SUBTILIS
Descriptor:CARBOXYLESTERASE YBFK, SODIUM ION
Authors:Rozeboom, H.J., Godinho, L.F., Nardini, M., Quax, W.J., Dijkstra, B.W.
Deposit date:2013-10-29
Release date:2014-01-22
Last modified:2017-07-05
Method:X-RAY DIFFRACTION (2.04 Å)
Cite:Crystal Structures of Two Bacillus Carboxylesterases with Different Enantioselectivities.
Biochim.Biophys.Acta, 1844, 2014
1KB0
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CRYSTAL STRUCTURE OF QUINOHEMOPROTEIN ALCOHOL DEHYDROGENASE FROM COMAMONAS TESTOSTERONI
Descriptor:quinohemoprotein alcohol dehydrogenase, CALCIUM ION, HEME C, ...
Authors:Rozeboom, H.J., Oubrie, A.
Deposit date:2001-11-05
Release date:2001-12-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.44 Å)
Cite:Crystal structure of quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni: structural basis for substrate oxidation and electron transfer.
J.Biol.Chem., 277, 2002
1KQY
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HEVAMINE MUTANT D125A/E127A/Y183F IN COMPLEX WITH PENTA-NAG
Descriptor:Hevamine A, N-ACETYL-D-GLUCOSAMINE, SULFATE ION, ...
Authors:Rozeboom, H.J., Dijkstra, B.W.
Deposit date:2002-01-08
Release date:2002-01-23
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.92 Å)
Cite:Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis.
Eur.J.Biochem., 269, 2002
1KQZ
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HEVAMINE MUTANT D125A/E127A/Y183F IN COMPLEX WITH TETRA-NAG
Descriptor:Hevamine A, N-ACETYL-D-GLUCOSAMINE
Authors:Rozeboom, H.J., Dijkstra, B.W.
Deposit date:2002-01-08
Release date:2002-01-23
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.92 Å)
Cite:Expression and Characterization of Active Site Mutants of Hevamine, a Chitinase from the Rubber Tree Hevea brasiliensis.
Eur.J.Biochem., 269, 2002
1KR0
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HEVAMINE MUTANT D125A/Y183F IN COMPLEX WITH TETRA-NAG
Descriptor:Hevamine A, N-ACETYL-D-GLUCOSAMINE, SULFATE ION
Authors:Rozeboom, H.J., Dijkstra, B.W.
Deposit date:2002-01-08
Release date:2002-01-23
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.92 Å)
Cite:Expression and Characterization of Active Site Mutants of Hevamine, a Chitinase from the Rubber Tree Hevea brasiliensis.
Eur.J.Biochem., 269, 2002
1KR1
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HEVAMINE MUTANT D125A/E127A IN COMPLEX WITH TETRA-NAG
Descriptor:Hevamine A, N-ACETYL-D-GLUCOSAMINE, SULFATE ION
Authors:Rozeboom, H.J., Dijkstra, B.W.
Deposit date:2002-01-08
Release date:2002-01-23
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2 Å)
Cite:Expression and Characterization of Active Site Mutants of Hevamine, a Chitinase from the Rubber Tree Hevea brasiliensis.
Eur.J.Biochem., 269, 2002
3BMV
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CYCLODEXTRIN GLYCOSYL TRANSFERASE FROM THERMOANEROBACTERIUM THERMOSULFURIGENES EM1 MUTANT S77P
Descriptor:Cyclomaltodextrin glucanotransferase, CALCIUM ION, SULFATE ION, ...
Authors:Rozeboom, H.J., van Oosterwijk, N., Dijkstra, B.W.
Deposit date:2007-12-13
Release date:2008-05-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Elimination of competing hydrolysis and coupling side reactions of a cyclodextrin glucanotransferase by directed evolution.
Biochem.J., 413, 2008
3BMW
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CYCLODEXTRIN GLYCOSYL TRANSFERASE FROM THERMOANEROBACTERIUM THERMOSULFURIGENES EM1 MUTANT S77P COMPLEXED WITH A MALTOHEPTAOSE INHIBITOR
Descriptor:Cyclomaltodextrin glucanotransferase, ALPHA-D-GLUCOSE, 6-DEOXY-ALPHA-D-GLUCOSE, ...
Authors:Rozeboom, H.J., van Oosterwijk, N., Dijkstra, B.W.
Deposit date:2007-12-13
Release date:2008-05-27
Last modified:2017-10-25
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Elimination of competing hydrolysis and coupling side reactions of a cyclodextrin glucanotransferase by directed evolution.
Biochem.J., 413, 2008
5OX2
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CRYSTAL STRUCTURE OF THYMOLIGASE, A SUBSTRATE-TAILORED PEPTILIGASE VARIANT
Descriptor:Subtilisin BPN', Fragment of prodomain, SULFATE ION
Authors:Rozeboom, H.J., Janssen, D.B.
Deposit date:2017-09-05
Release date:2018-01-10
Last modified:2018-04-18
Method:X-RAY DIFFRACTION (2.24 Å)
Cite:Design of a substrate-tailored peptiligase variant for the efficient synthesis of thymosin-alpha1.
Org. Biomol. Chem., 16, 2018
1KCK
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BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYL TRANSFERASE MUTANT N193G
Descriptor:CYCLODEXTRIN GLYCOSYLTRANSFERASE, ALPHA-D-GLUCOSE, 6-DEOXY-ALPHA-D-GLUCOSE, ...
Authors:Rozeboom, H.J., Uitdehaag, J.C.M., Dijkstra, B.W.
Deposit date:2001-11-09
Release date:2002-01-16
Last modified:2017-10-25
Method:X-RAY DIFFRACTION (2.43 Å)
Cite:The remote substrate binding subsite -6 in cyclodextrin-glycosyltransferase controls the transferase activity of the enzyme via an induced-fit mechanism.
J.Biol.Chem., 277, 2002
1KCL
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BACILLUS CIRUCLANS STRAIN 251 CYCLODEXTRIN GLYCOSYL TRANSFERASE MUTANT G179L
Descriptor:Cyclodextrin glycosyltransferase, MALTOSE, ALPHA-D-GLUCOSE, ...
Authors:Rozeboom, H.J., Uitdehaag, J.C.M., Dijkstra, B.W.
Deposit date:2001-11-09
Release date:2002-01-16
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (1.94 Å)
Cite:The remote substrate binding subsite -6 in cyclodextrin-glycosyltransferase controls the transferase activity of the enzyme via an induced-fit mechanism.
J.Biol.Chem., 277, 2002
1OT1
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BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYL TRANSFERASE MUTANT D135A
Descriptor:Cyclomaltodextrin glucanotransferase, MALTOSE, BETA-D-GLUCOSE, ...
Authors:Rozeboom, H.J., Dijkstra, B.W.
Deposit date:2003-03-21
Release date:2003-06-03
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2 Å)
Cite:The fully conserved Asp residue in Conserved sequence region I of the alpha-amylase Family is crucial for the Catalytic Site Architecture and Activity
Febs Lett., 541, 2003
1OT2
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BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYL TRANSFERASE MUTANT D135N
Descriptor:Cyclomaltodextrin glucanotransferase, MALTOSE, BETA-D-GLUCOSE, ...
Authors:Rozeboom, H.J., Dijkstra, B.W.
Deposit date:2003-03-21
Release date:2003-06-03
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:The fully conserved Asp residue in Conserved sequence region I of the alpha-amylase Family is crucial for the Catalytic Site Architecture and Activity
Febs Lett., 541, 2003
1PEZ
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BACILLUS CIRCULANS STRAIN 251 MUTANT A230V
Descriptor:Cyclomaltodextrin glucanotransferase, MALTOSE, BETA-D-GLUCOSE, ...
Authors:Rozeboom, H.J., Dijkstra, B.W.
Deposit date:2003-05-23
Release date:2003-10-28
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.32 Å)
Cite:Conversion of Cyclodextrin Glycosyltransferase into a Starch Hydrolase by Directed Evolution: The Role of Alanine 230 in Acceptor Subsite +1
Biochemistry, 42, 2003