Author results

3VM4
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CYTOCHROME P450SP ALPHA (CYP152B1) IN COMPLEX WITH (R)-IBUPROPHEN
Descriptor:Fatty acid alpha-hydroxylase, PROTOPORPHYRIN IX CONTAINING FE, (2R)-2-[4-(2-methylpropyl)phenyl]propanoic acid, ...
Authors:Fujishiro, T., Shoji, O., Nagano, S., Sugimoto, H., Shiro, Y., Watanabe, Y.
Deposit date:2011-12-08
Release date:2012-05-09
Last modified:2013-08-14
Method:X-RAY DIFFRACTION (1.94 Å)
Cite:Chiral-substrate-assisted stereoselective epoxidation catalyzed by H2O2-dependent cytochrome P450SP alpha
Chem Asian J, 7, 2012
5D4T
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SEMET-LABELLED HCGC FROM METHANOCALDOCOCCUS JANNASCHII IN SPACE GROUP P212121
Descriptor:Uncharacterized protein MJ0489
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2015-08-09
Release date:2016-07-20
Last modified:2016-08-10
Method:X-RAY DIFFRACTION (2.9 Å)
Cite:Identification of HcgC as a SAM-Dependent Pyridinol Methyltransferase in [Fe]-Hydrogenase Cofactor Biosynthesis.
Angew.Chem.Int.Ed.Engl., 55, 2016
5D4U
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SAM-BOUND HCGC FROM METHANOCALDOCOCCUS JANNASCHII
Descriptor:Uncharacterized protein MJ0489, S-ADENOSYLMETHIONINE, SULFATE ION
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2015-08-09
Release date:2016-07-20
Last modified:2016-08-10
Method:X-RAY DIFFRACTION (2 Å)
Cite:Identification of HcgC as a SAM-Dependent Pyridinol Methyltransferase in [Fe]-Hydrogenase Cofactor Biosynthesis.
Angew.Chem.Int.Ed.Engl., 55, 2016
5D4V
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HCGC WITH SAH AND A GUANYLYLPYRIDINOL (GP) DERIVATIVE
Descriptor:Uncharacterized protein MJ0489, S-ADENOSYL-L-HOMOCYSTEINE, SULFATE ION, ...
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2015-08-09
Release date:2016-07-20
Last modified:2016-08-10
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Identification of HcgC as a SAM-Dependent Pyridinol Methyltransferase in [Fe]-Hydrogenase Cofactor Biosynthesis.
Angew.Chem.Int.Ed.Engl., 55, 2016
5D5O
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HCGC FROM METHANOCALDOCOCCUS JANNASCHII
Descriptor:Uncharacterized protein MJ0489, SULFATE ION
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2015-08-11
Release date:2016-07-20
Last modified:2016-08-10
Method:X-RAY DIFFRACTION (2.7 Å)
Cite:Identification of HcgC as a SAM-Dependent Pyridinol Methyltransferase in [Fe]-Hydrogenase Cofactor Biosynthesis.
Angew.Chem.Int.Ed.Engl., 55, 2016
5D5T
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SEMET-LABELLED HCGC FROM METHANOCALDOCOCCUS JANNASCHII IN P1 SPACE GROUP
Descriptor:Uncharacterized protein MJ0489
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2015-08-11
Release date:2016-07-20
Last modified:2016-08-10
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:Identification of HcgC as a SAM-Dependent Pyridinol Methyltransferase in [Fe]-Hydrogenase Cofactor Biosynthesis.
Angew.Chem.Int.Ed.Engl., 55, 2016
5WT4
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L-CYSTEINE-PLP INTERMEDIATE OF NIFS FROM HELICOBACTER PYLORI
Descriptor:Cysteine desulfurase IscS, ISOPROPYL ALCOHOL, N-({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)-L-CYSTEINE
Authors:Fujishiro, T., Nakamura, R., Takahashi, T.
Deposit date:2016-12-09
Release date:2017-12-13
Method:X-RAY DIFFRACTION (2.92 Å)
Cite:Structural snapshot of cysteine desulfurase NifS with L-cysteine in initiation of catalysis
To Be Published
5XT5
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SUFS-SUFU COMPLEX FROM BACILLUS SUBTILIS
Descriptor:Zinc-dependent sulfurtransferase SufU, Cysteine desulfurase SufS, ZINC ION, ...
Authors:Fujishiro, T., Takahashi, Y.
Deposit date:2017-06-17
Release date:2017-12-20
Last modified:2018-01-17
Method:X-RAY DIFFRACTION (2.34 Å)
Cite:Zinc-Ligand Swapping Mediated Complex Formation and Sulfur Transfer between SufS and SufU for Iron-Sulfur Cluster Biogenesis in Bacillus subtilis
J. Am. Chem. Soc., 139, 2017
5XT6
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A SULFUR-TRANSFERRING CATALYTIC INTERMEDIATE OF SUFS-SUFU COMPLEX FROM BACILLUS SUBTILIS
Descriptor:Zinc-dependent sulfurtransferase SufU, Cysteine desulfurase SufS, ZINC ION, ...
Authors:Fujishiro, T., Kunichika, K., Takahashi, Y.
Deposit date:2017-06-17
Release date:2017-12-20
Last modified:2018-01-17
Method:X-RAY DIFFRACTION (3.5 Å)
Cite:Zinc-Ligand Swapping Mediated Complex Formation and Sulfur Transfer between SufS and SufU for Iron-Sulfur Cluster Biogenesis in Bacillus subtilis
J. Am. Chem. Soc., 139, 2017
3AWM
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CYTOCHROME P450SP ALPHA (CYP152B1) WILD-TYPE WITH PALMITIC ACID
Descriptor:Fatty acid alpha-hydroxylase, PROTOPORPHYRIN IX CONTAINING FE, PALMITIC ACID, ...
Authors:Fujishiro, T., Shoji, O., Nagano, S., Sugimoto, H., Shiro, Y., Watanabe, Y.
Deposit date:2011-03-25
Release date:2011-06-29
Last modified:2013-06-05
Method:X-RAY DIFFRACTION (1.65 Å)
Cite:Crystal structure of H2O2-dependent cytochrome P450SPalpha with its bound fatty acid substrate: insight into the regioselective hydroxylation of fatty acids at the alpha position
J.Biol.Chem., 286, 2011
3AWP
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CYTOCHROME P450SP ALPHA (CYP152B1) MUTANT F288G
Descriptor:Fatty acid alpha-hydroxylase, PROTOPORPHYRIN IX CONTAINING FE, PALMITIC ACID, ...
Authors:Fujishiro, T., Shoji, O., Nagano, S., Sugimoto, H., Shiro, Y., Watanabe, Y.
Deposit date:2011-03-25
Release date:2011-06-29
Last modified:2013-06-05
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Crystal structure of H2O2-dependent cytochrome P450SPalpha with its bound fatty acid substrate: insight into the regioselective hydroxylation of fatty acids at the alpha position.
J.Biol.Chem., 286, 2011
3AWQ
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CYTOCHROME P450SP ALPHA (CYP152B1) MUTANT L78F
Descriptor:Fatty acid alpha-hydroxylase, PROTOPORPHYRIN IX CONTAINING FE, PALMITIC ACID
Authors:Fujishiro, T., Shoji, O., Nagano, S., Sugimoto, H., Shiro, Y., Watanabe, Y.
Deposit date:2011-03-25
Release date:2011-06-29
Last modified:2013-06-05
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Crystal structure of H2O2-dependent cytochrome P450SPalpha with its bound fatty acid substrate: insight into the regioselective hydroxylation of fatty acids at the alpha position.
J.Biol.Chem., 286, 2011
3WB0
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HCGB FROM METHANOCALDOCOCCUS JANNASCHII IN COMPLEX WITH LIGHT-DECOMPOSED FEGP COFACTOR OF [FE]-HYDROGENASE
Descriptor:Uncharacterized protein MJ0488, 5'-O-[(S)-{[2-(carboxymethyl)-6-hydroxy-3,5-dimethylpyridin-4-yl]oxy}(hydroxy)phosphoryl]guanosine
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2013-05-10
Release date:2014-02-05
Method:X-RAY DIFFRACTION (1.91 Å)
Cite:Identification of the HcgB enzyme in [Fe]-hydrogenase-cofactor biosynthesis.
Angew.Chem.Int.Ed.Engl., 52, 2013
3WB2
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HCGB FROM METHANOCALDOCOCCUS JANNASCHII IN COMPLEX WITH THE GUANYLYL-PYRIDINOL PRODUCT IN A MODEL REACTION OF [FE]-HYDROGENASE COFACTOR BIOSYNTHESIS
Descriptor:Uncharacterized protein MJ0488, 5'-O-[(R)-[(3,6-dimethyl-2-oxo-1,2-dihydropyridin-4-yl)oxy](hydroxy)phosphoryl]guanosine, MAGNESIUM ION, ...
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2013-05-11
Release date:2014-02-05
Method:X-RAY DIFFRACTION (2.44 Å)
Cite:Identification of the HcgB enzyme in [Fe]-hydrogenase-cofactor biosynthesis.
Angew.Chem.Int.Ed.Engl., 52, 2013
3WSD
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OXIDIZED HCGD FROM METHANOCALDOCOCCUS JANNASCHII
Descriptor:Putative GTP cyclohydrolase 1 type 2, FE (III) ION, CHLORIDE ION
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-03-13
Release date:2014-08-27
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:A possible iron delivery function of the dinuclear iron center of HcgD in [Fe]-hydrogenase cofactor biosynthesis
Febs Lett., 588, 2014
3WSE
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REDUCED HCGD FROM METHANOCALDOCOCCUS JANNASCHII
Descriptor:Putative GTP cyclohydrolase 1 type 2, FE (II) ION, PHOSPHATE ION
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-03-13
Release date:2014-08-27
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:A possible iron delivery function of the dinuclear iron center of HcgD in [Fe]-hydrogenase cofactor biosynthesis
Febs Lett., 588, 2014
3WSF
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OXIDIZED HCGD FROM METHANOCALDOCOCCUS JANNASCHII WITH CITRATE
Descriptor:Putative GTP cyclohydrolase 1 type 2, FE (III) ION, CITRIC ACID
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-03-13
Release date:2014-08-27
Method:X-RAY DIFFRACTION (2 Å)
Cite:A possible iron delivery function of the dinuclear iron center of HcgD in [Fe]-hydrogenase cofactor biosynthesis
Febs Lett., 588, 2014
3WSG
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REDUCED HCGD FROM METHANOCALDOCOCCUS JANNASCHII WITH CITRATE
Descriptor:Putative GTP cyclohydrolase 1 type 2, FE (II) ION, CITRIC ACID
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-03-13
Release date:2014-08-27
Method:X-RAY DIFFRACTION (1.996 Å)
Cite:A possible iron delivery function of the dinuclear iron center of HcgD in [Fe]-hydrogenase cofactor biosynthesis
Febs Lett., 588, 2014
3WSH
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EDTA-TREATED, OXIDIZED HCGD FROM METHANOCALDOCOCCUS JANNASCHII
Descriptor:Putative GTP cyclohydrolase 1 type 2, FE (III) ION, SULFATE ION
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-03-13
Release date:2014-08-27
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:A possible iron delivery function of the dinuclear iron center of HcgD in [Fe]-hydrogenase cofactor biosynthesis
Febs Lett., 588, 2014
3WSI
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EDTA-TREATED, REDUCED HCGD FROM METHANOCALDOCOCCUS JANNASCHII
Descriptor:Putative GTP cyclohydrolase 1 type 2, FE (II) ION, PHOSPHATE ION
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-03-13
Release date:2014-08-27
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:A possible iron delivery function of the dinuclear iron center of HcgD in [Fe]-hydrogenase cofactor biosynthesis
Febs Lett., 588, 2014
3WV7
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HCGE FROM METHANOTHERMOBACTER MARBURGENSIS
Descriptor:Hmd co-occurring protein HcgE, ADENOSINE-5'-DIPHOSPHATE
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-05-16
Release date:2015-04-29
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Protein-pyridinol thioester precursor for biosynthesis of the organometallic acyl-iron ligand in [Fe]-hydrogenase cofactor
Nat Commun, 6, 2015
3WV8
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ATP-BOUND HCGE FROM METHANOTHERMOBACTER MARBURGENSIS
Descriptor:Hmd co-occurring protein HcgE, ADENOSINE-5'-TRIPHOSPHATE, SULFATE ION
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-05-16
Release date:2015-04-29
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Protein-pyridinol thioester precursor for biosynthesis of the organometallic acyl-iron ligand in [Fe]-hydrogenase cofactor
Nat Commun, 6, 2015
3WV9
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GUANYLYLPYRIDINOL (GP)- AND ATP-BOUND HCGE FROM METHANOTHERMOBACTER MARBURGENSIS
Descriptor:Hmd co-occurring protein HcgE, ADENOSINE-5'-TRIPHOSPHATE, 5'-O-[(S)-{[2-(carboxymethyl)-6-hydroxy-3,5-dimethylpyridin-4-yl]oxy}(hydroxy)phosphoryl]guanosine, ...
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-05-16
Release date:2015-04-29
Method:X-RAY DIFFRACTION (2.75 Å)
Cite:Protein-pyridinol thioester precursor for biosynthesis of the organometallic acyl-iron ligand in [Fe]-hydrogenase cofactor
Nat Commun, 6, 2015
3WVA
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SEMET-LABELLED HCGF FROM METHANOCALDOCOCCUS JANNASCHII
Descriptor:UPF0254 protein MJ1251
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-05-16
Release date:2015-04-29
Method:X-RAY DIFFRACTION (1.4 Å)
Cite:Protein-pyridinol thioester precursor for biosynthesis of the organometallic acyl-iron ligand in [Fe]-hydrogenase cofactor
Nat Commun, 6, 2015
3WVB
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HCGF FROM METHANOCALDOCOCCUS JANNASCHII
Descriptor:UPF0254 protein MJ1251
Authors:Fujishiro, T., Ermler, U., Shima, S.
Deposit date:2014-05-16
Release date:2015-04-29
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Protein-pyridinol thioester precursor for biosynthesis of the organometallic acyl-iron ligand in [Fe]-hydrogenase cofactor
Nat Commun, 6, 2015
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