7Z4D
 
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7Z4E
 | | SpCas9 bound to 8-nucleotide complementary DNA substrate | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target strand of 8 nucleotide complementary DNA substrate, Target strand of 8 nucleotide complementary DNA substrate, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-03-03 | | Release date: | 2022-08-31 | | Last modified: | 2022-09-14 | | Method: | ELECTRON MICROSCOPY (4.14 Å) | | Cite: | R-loop formation and conformational activation mechanisms of Cas9.
Nature, 609, 2022
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7Z4K
 | | SpCas9 bound to 10-nucleotide complementary DNA substrate | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target strand of 10-nucleotide complementary DNA substrate, Target strand of 10-nucleotide complementary DNA substrate, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-03-04 | | Release date: | 2022-08-31 | | Last modified: | 2022-09-14 | | Method: | ELECTRON MICROSCOPY (3.81 Å) | | Cite: | R-loop formation and conformational activation mechanisms of Cas9.
Nature, 609, 2022
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7Z4G
 | | SpCas9 bound to 12-nucleotide complementary DNA substrate | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target strand of 12-nucleotide complementary DNA substrate, Target strand of 12-nucleotide complementary DNA substrate, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-03-03 | | Release date: | 2022-08-31 | | Last modified: | 2022-09-14 | | Method: | ELECTRON MICROSCOPY (3.64 Å) | | Cite: | R-loop formation and conformational activation mechanisms of Cas9.
Nature, 609, 2022
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7Z4L
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7Z4C
 | | SpCas9 bound to 6 nucleotide complementary DNA substrate | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target strand of 6 nucleotide complementary DNA substrate, Target strand of 6 nucleotide complementary DNA substrate, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-03-03 | | Release date: | 2022-08-31 | | Last modified: | 2022-09-14 | | Method: | ELECTRON MICROSCOPY (3.87 Å) | | Cite: | R-loop formation and conformational activation mechanisms of Cas9.
Nature, 609, 2022
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7Z4I
 | | SpCas9 bound to 16-nucleotide complementary DNA substrate | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target strand of 16-nucleotide complementary DNA substrate, POTASSIUM ION, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-03-03 | | Release date: | 2022-08-31 | | Last modified: | 2022-09-14 | | Method: | ELECTRON MICROSCOPY (3.12 Å) | | Cite: | R-loop formation and conformational activation mechanisms of Cas9.
Nature, 609, 2022
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7Z4H
 | | SpCas9 bound to 14-nucleotide complementary DNA substrate | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, Non-target strand of 14-nucleotide complementary DNA substrate, Target strand of 14-nucleotide complementary DNA substrate, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-03-03 | | Release date: | 2022-08-31 | | Last modified: | 2022-09-14 | | Method: | ELECTRON MICROSCOPY (3.49 Å) | | Cite: | R-loop formation and conformational activation mechanisms of Cas9.
Nature, 609, 2022
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7Z4J
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7OX9
 | | Target-bound SpCas9 complex with AAVS1 all-RNA guide | | Descriptor: | AAVS1 non-target DNA strand, AAVS1 target DNA strand, CRISPR-associated endonuclease Cas9/Csn1, ... | | Authors: | Pacesa, M, Donohoue, P, May, A.P, Jinek, M, Cameron, P. | | Deposit date: | 2021-06-22 | | Release date: | 2021-09-15 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2.45 Å) | | Cite: | Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells.
Mol.Cell, 81, 2021
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8QHP
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7ZO1
 | | SpCas9 bound to CD34 off-target9 DNA substrate | | Descriptor: | CD34 off-target9 DNA non-target strand, CD34 off-target9 DNA target strand, CD34 sgRNA, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-04-23 | | Release date: | 2022-10-26 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2.4 Å) | | Cite: | Structural basis for Cas9 off-target activity.
Cell, 185, 2022
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8OYW
 | | De novo designed rhomboid protease-like fold RPF_9 | | Descriptor: | De novo designed soluble Rhomboid protease-like protein, SODIUM ION | | Authors: | Pacesa, M, Correia, B.E. | | Deposit date: | 2023-05-05 | | Release date: | 2023-10-18 | | Last modified: | 2024-03-27 | | Method: | X-RAY DIFFRACTION (1.5 Å) | | Cite: | Computational design of soluble functional analogues of integral membrane proteins.
Biorxiv, 2024
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8OYY
 | | De novo designed soluble GPCR-like fold GLF_32 | | Descriptor: | CHLORIDE ION, De novo designed soluble GPCR-like protein, POTASSIUM ION | | Authors: | Pacesa, M, Correia, B.E. | | Deposit date: | 2023-05-05 | | Release date: | 2023-10-18 | | Last modified: | 2024-03-27 | | Method: | X-RAY DIFFRACTION (1.85 Å) | | Cite: | Computational design of soluble functional analogues of integral membrane proteins.
Biorxiv, 2024
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8OYV
 | | De novo designed Claudin fold CLF_4 | | Descriptor: | De novo designed soluble Claudin | | Authors: | Pacesa, M, Correia, B.E. | | Deposit date: | 2023-05-05 | | Release date: | 2023-10-18 | | Last modified: | 2024-03-27 | | Method: | X-RAY DIFFRACTION (2.79 Å) | | Cite: | Computational design of soluble functional analogues of integral membrane proteins.
Biorxiv, 2024
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8OYS
 | | De novo designed TIM barrel fold TBF_24 | | Descriptor: | CHLORIDE ION, De novo designed TIM-barrel | | Authors: | Pacesa, M, Correia, B.E. | | Deposit date: | 2023-05-05 | | Release date: | 2023-10-18 | | Last modified: | 2024-03-27 | | Method: | X-RAY DIFFRACTION (1.34 Å) | | Cite: | Computational design of soluble functional analogues of integral membrane proteins.
Biorxiv, 2024
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8OYX
 | | De novo designed soluble GPCR-like fold GLF_18 | | Descriptor: | De novo designed soluble GPCR-like protein, PHOSPHATE ION | | Authors: | Pacesa, M, Correia, B.E. | | Deposit date: | 2023-05-05 | | Release date: | 2023-10-18 | | Last modified: | 2024-03-27 | | Method: | X-RAY DIFFRACTION (2.11 Å) | | Cite: | Computational design of soluble functional analogues of integral membrane proteins.
Biorxiv, 2024
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8Q70
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7QQQ
 | | SpCas9 bound to AAVS1 off-target4 DNA substrate | | Descriptor: | 1,2-ETHANEDIOL, AAVS1 off-target4 non-target strand, AAVS1 off-target4 target strand, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-01-10 | | Release date: | 2022-10-26 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2.65 Å) | | Cite: | Structural basis for Cas9 off-target activity.
Cell, 185, 2022
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7QQV
 | | SpCas9 bound to FANCF off-target3 DNA substrate | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target3 non-target strand, FANCF off-target3 target strand, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-01-10 | | Release date: | 2022-10-26 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2.25 Å) | | Cite: | Structural basis for Cas9 off-target activity.
Cell, 185, 2022
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7QR8
 | | SpCas9 bound to PTPRC off-target1 DNA substrate | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, MAGNESIUM ION, POTASSIUM ION, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-01-10 | | Release date: | 2022-10-26 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2.75 Å) | | Cite: | Structural basis for Cas9 off-target activity.
Cell, 185, 2022
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7QQO
 | | SpCas9 bound to AAVS1 off-target1 DNA substrate | | Descriptor: | AAVS1 off-target1 non-target strand, AAVS1 off-target1 target strand, AAVS1 sgRNA, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-01-10 | | Release date: | 2022-10-26 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (3 Å) | | Cite: | Structural basis for Cas9 off-target activity.
Cell, 185, 2022
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7QQW
 | | SpCas9 bound to FANCF off-target4 DNA substrate | | Descriptor: | CRISPR-associated endonuclease Cas9/Csn1, FANCF off-target4 non-target strand, FANCF off-target4 target strand, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-01-10 | | Release date: | 2022-10-26 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (3.1 Å) | | Cite: | Structural basis for Cas9 off-target activity.
Cell, 185, 2022
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7QQR
 | | SpCas9 bound to AAVS1 off-target5 DNA substrate | | Descriptor: | 1,2-ETHANEDIOL, AAVS1 off-target5 non-target strand, AAVS1 off-target5 target strand, ... | | Authors: | Pacesa, M, Jinek, M. | | Deposit date: | 2022-01-10 | | Release date: | 2022-10-26 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (2.75 Å) | | Cite: | Structural basis for Cas9 off-target activity.
Cell, 185, 2022
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7QR7
 | | SpCas9 bound to AAVS1 off-target2 DNA substrate | | Descriptor: | AAVS1 off-target2 non-target strand, AAVS1 off-target2 target strand, AAVS1 sgRNA, ... | | Authors: | Pacesa, M, JInek, M. | | Deposit date: | 2022-01-10 | | Release date: | 2022-10-26 | | Last modified: | 2024-01-31 | | Method: | X-RAY DIFFRACTION (3 Å) | | Cite: | Structural basis for Cas9 off-target activity.
Cell, 185, 2022
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