7Z4D
Crystal structure of SpCas9 bound to a 10 nucleotide complementary DNA substrate
Summary for 7Z4D
| Entry DOI | 10.2210/pdb7z4d/pdb |
| Descriptor | sgRNA, CRISPR-associated endonuclease Cas9/Csn1, Target strand of 10 nucleotide complementary DNA substrate, ... (6 entities in total) |
| Functional Keywords | crispr, cas9, r-loop, substrate binding, off-target, hydrolase |
| Biological source | Streptococcus pyogenes More |
| Total number of polymer chains | 8 |
| Total formula weight | 409031.44 |
| Authors | Pacesa, M.,Jinek, M. (deposition date: 2022-03-03, release date: 2022-08-31, Last modification date: 2024-01-31) |
| Primary citation | Pacesa, M.,Loeff, L.,Querques, I.,Muckenfuss, L.M.,Sawicka, M.,Jinek, M. R-loop formation and conformational activation mechanisms of Cas9. Nature, 609:191-196, 2022 Cited by PubMed: 36002571DOI: 10.1038/s41586-022-05114-0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.1 Å) |
Structure validation
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