8T6S

SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 10 bp R-loop

Summary for 8T6S

Entry DOI	10.2210/pdb8t6s/pdb
EMDB information	41079
Descriptor	CRISPR-associated endonuclease Cas9/Csn1, gRNA, TS, ... (4 entities in total)
Functional Keywords	spry-cas9, crispr, cas9, immune system, r-loop
Biological source	Streptococcus pyogenes More
Total number of polymer chains	4
Total formula weight	197081.53
Authors	Hibshman, G.N.,Bravo, J.P.K.,Taylor, D.W. (deposition date: 2023-06-18, release date: 2024-05-01, Last modification date: 2024-05-15)
Primary citation	Hibshman, G.N.,Bravo, J.P.K.,Hooper, M.M.,Dangerfield, T.L.,Zhang, H.,Finkelstein, I.J.,Johnson, K.A.,Taylor, D.W. Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9. Nat Commun, 15:3663-3663, 2024 Cited by PubMed: 38688943 DOI: 10.1038/s41467-024-47830-3 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.28 Å)

Structure validation