8J12

Cryo-EM structure of the AsCas12f-sgRNA-target DNA ternary complex

Summary for 8J12

• Entry DOI: 10.2210/pdb8j12/pdb
• EMDB information: 35912
• Descriptor: Transposase IS605 OrfB C-terminal domain-containing protein, DNA (38-MER), RNA (247-MER), ... (6 entities in total)
• Functional Keywords: crispr-cas, rna binding protein-dna complex, rna binding protein, rna binding protein-dna-rna complex, rna binding protein/dna/rna
• Biological source: Acidibacillus sulfuroxidans; More
• Total number of polymer chains: 5
• Total formula weight: 195864.96

Authors

Hino, T.,Omura, N.S.,Nakagawa, R.,Togashi, T.,Takeda, N.S.,Hiramoto, T.,Tasaka, S.,Hirano, H.,Tokuyama, T.,Uosaki, H.,Ishiguro, H.,Yamano, H.,Ozaki, Y.,Motooka, D.,Mori, H.,Kirita, Y.,Kise, Y.,Itoh, Y.,Matoba, S.,Aburatani, H.,Yachie, N.,Siksnys, V.,Ohmori, T.,Hoshino, A.,Nureki, O. (deposition date: 2023-04-12, release date: 2023-09-27)

• Primary citation: Hino, T.,Omura, N.S.,Nakagawa, R.,Togashi, T.,Takeda, N.S.,Hiramoto, T.,Tasaka, S.,Hirano, H.,Tokuyama, T.,Uosaki, H.,Ishiguro, H.,Yamano, H.,Ozaki, Y.,Motooka, D.,Mori, H.,Kirita, Y.,Kise, Y.,Itoh, Y.,Matoba, S.,Aburatani, H.,Yachie, N.,Siksnys, V.,Ohmori, T.,Hoshino, A.,Nureki, O.; Minimal and most efficient genome editing Cas enzyme; To Be Published,

Experimental method

ELECTRON MICROSCOPY (3.08 Å)