8IN8

Cryo-EM structure of the target ssDNA-bound SIR2-APAZ/Ago-gRNA quaternary complex

Summary for 8IN8

• Entry DOI: 10.2210/pdb8in8/pdb
• EMDB information: 35592
• Descriptor: Sir2 superfamily protein, Piwi domain protein, RNA (5'-R(P*AP*UP*AP*AP*UP*GP*GP*UP*UP*UP*CP*UP*UP*AP*GP*AP*CP*GP*UP*CP*GP*UP*UP*U)-3'), ... (5 entities in total)
• Functional Keywords: a protein, antiviral protein
• Biological source: Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA); More
• Total number of polymer chains: 4
• Total formula weight: 134656.88

Authors

Zhang, H.,Li, Z.,Yu, G.M.,Li, X.Z.,Wang, X.S. (deposition date: 2023-03-08, release date: 2023-07-05, Last modification date: 2023-09-13)

• Primary citation: Wang, X.,Li, X.,Yu, G.,Zhang, L.,Zhang, C.,Wang, Y.,Liao, F.,Wen, Y.,Yin, H.,Liu, X.,Wei, Y.,Li, Z.,Deng, Z.,Zhang, H.; Structural insights into mechanisms of Argonaute protein-associated NADase activation in bacterial immunity.; Cell Res., 33:699-711, 2023; PubMed: 37311833; DOI: 10.1038/s41422-023-00839-7

Experimental method

ELECTRON MICROSCOPY (3 Å)