8HJ4

CryoEM structure of an anti-CRISPR protein AcrIIC5 bound to Nme1Cas9-sgRNA complex

Summary for 8HJ4

• Entry DOI: 10.2210/pdb8hj4/pdb
• EMDB information: 34832
• Descriptor: CRISPR-associated endonuclease Cas9, sgRNA, Phage protein (3 entities in total)
• Functional Keywords: cas9, anti-crispr proteins, cleavage inhibition, antimicrobial protein, hydrolase-rna-antimicrobial protein complex, hydrolase/rna/antimicrobial protein
• Biological source: Neisseria meningitidis serogroup C (strain 8013); More
• Total number of polymer chains: 3
• Total formula weight: 183160.15

Authors

Wang, Y.,Sun, W. (deposition date: 2022-11-22, release date: 2023-01-25, Last modification date: 2023-03-15)

• Primary citation: Sun, W.,Zhao, X.,Wang, J.,Yang, X.,Cheng, Z.,Liu, S.,Wang, J.,Sheng, G.,Wang, Y.; Anti-CRISPR AcrIIC5 is a dsDNA mimic that inhibits type II-C Cas9 effectors by blocking PAM recognition.; Nucleic Acids Res., 51:1984-1995, 2023; PubMed: 36744495; DOI: 10.1093/nar/gkad052

Experimental method

ELECTRON MICROSCOPY (3.1 Å)