8D8M

Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence grown in a calcium chloride crystallization solution.

Summary for 8D8M

• Entry DOI: 10.2210/pdb8d8m/pdb
• Related: 7u6k
• Descriptor: DNA (5'-D(CP*CP*CP*GP*GP*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*AP*CP*GP*GP*A)-3'), DNA (5'-D(GP*CP*CP*GP*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*GP*GP*A)-3'), Replication initiation protein, ... (5 entities in total)
• Functional Keywords: replication initiator repe complex co-crystal, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Escherichia coli K-12; More
• Total number of polymer chains: 3
• Total formula weight: 50523.87

Authors

Orun, A.R.,Snow, C.D. (deposition date: 2022-06-08, release date: 2023-06-28, Last modification date: 2023-10-25)

• Primary citation: Orun, A.R.,Shields, E.T.,Dmytriw, S.,Vajapayajula, A.,Snow, C.D.; Designed crystalline assemblies of protein and DNA enable site-specific installation of a guest protein, DNA, and small molecule.; To Be Published,

Experimental method

X-RAY DIFFRACTION (3.1 Å)