7YQS

Neutron structure of a L-rhamnose-alpha-1,4-D-glucuronate lyase from Fusarium oxysporum 12S, L-Rha complex

Summary for 7YQS

• Entry DOI: 10.2210/pdb7yqs/pdb
• Descriptor: L-RHAMNOSE-ALPHA-1,4-D-GLUCURONATE LYASE, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-L-rhamnopyranose, ... (8 entities in total)
• Functional Keywords: neutron diffraction crystallography, polysaccharide lyase, gum arabic, carbohydrate-active enzyme, lyase
• Biological source: Fusarium oxysporum
• Total number of polymer chains: 1
• Total formula weight: 51728.72

Authors

Yano, N.,Kondo, T.,Kusaka, K.,Yamada, T.,Arakawa, T.,Sakamoto, T.,Fushinobu, S. (deposition date: 2022-08-08, release date: 2023-08-09, Last modification date: 2024-03-27)

• Primary citation: Yano, N.,Kondo, T.,Kusaka, K.,Arakawa, T.,Sakamoto, T.,Fushinobu, S.; Charge neutralization and beta-elimination cleavage mechanism of family 42 L-rhamnose-alpha-1,4-D-glucuronate lyase revealed using neutron crystallography.; J.Biol.Chem., 300:105774-105774, 2024; PubMed: 38382672; DOI: 10.1016/j.jbc.2024.105774

Experimental method

NEUTRON DIFFRACTION (1.8 Å)
X-RAY DIFFRACTION (1.25 Å)