7YKB

Neutron Structure of PcyA D105N Mutant Complexed with Biliverdin at Room Temperature

Summary for 7YKB

• Entry DOI: 10.2210/pdb7ykb/pdb
• Related: 3F0L 3F0M 4QCD 7YK9
• Descriptor: Phycocyanobilin:ferredoxin oxidoreductase, 3-[5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-2-[[5-[(Z)-(3-ethenyl-4-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1H-pyrrol-2-yl]methyl]-4-methyl-1H-pyrrol-3-yl]propanoic acid, SODIUM ION, ... (4 entities in total)
• Functional Keywords: phycocyanobilin, ferredoxin oxi-doreductase, neutron, hydrogen, proton, mutant, reaction, absorption spectrum, oxidoreductase
• Biological source: Synechocystis sp. PCC 6803 substr. Kazusa
• Total number of polymer chains: 1
• Total formula weight: 28762.82

Authors

Unno, M.,Nanasawa, R. (deposition date: 2022-07-22, release date: 2023-01-25, Last modification date: 2024-04-03)

• Primary citation: Joutsuka, T.,Nanasawa, R.,Igarashi, K.,Horie, K.,Sugishima, M.,Hagiwara, Y.,Wada, K.,Fukuyama, K.,Yano, N.,Mori, S.,Ostermann, A.,Kusaka, K.,Unno, M.; Neutron crystallography and quantum chemical analysis of bilin reductase PcyA mutants reveal substrate and catalytic residue protonation states.; J.Biol.Chem., 299:102763-102763, 2022; PubMed: 36463961; DOI: 10.1016/j.jbc.2022.102763

Experimental method

NEUTRON DIFFRACTION (2.1 Å)
X-RAY DIFFRACTION (1.38 Å)