7R3I

PROSS optimitzed variant of RhlR (61 mutations) in complex with the synthetic antagonist mBTL

Summary for 7R3I

• Entry DOI: 10.2210/pdb7r3i/pdb
• Descriptor: PROSS optimized variant of RhlR with 61 mutations, 4-(3-bromophenoxy)-N-[(3S)-2-oxothiolan-3-yl]butanamide (2 entities in total)
• Functional Keywords: quorum sensing, luxr-type transcriptional regulator, pseudomonas, dna binding protein, gene regulation, mbtl, rhamnolipid, transcription
• Biological source: Pseudomonas aeruginosa PAO1
• Total number of polymer chains: 2
• Total formula weight: 56189.19

Authors

Henke, S.,Blankenfeldt, W. (deposition date: 2022-02-07, release date: 2022-12-14)

• Primary citation: Borgert, S.R.,Henke, S.,Witzgall, F.,Schmelz, S.,Zur Lage, S.,Hotop, S.K.,Stephen, S.,Lubken, D.,Kruger, J.,Gomez, N.O.,van Ham, M.,Jansch, L.,Kalesse, M.,Pich, A.,Bronstrup, M.,Haussler, S.,Blankenfeldt, W.; Moonlighting chaperone activity of the enzyme PqsE contributes to RhlR-controlled virulence of Pseudomonas aeruginosa.; Nat Commun, 13:7402-7402, 2022; PubMed: 36456567; DOI: 10.1038/s41467-022-35030-w

Experimental method

X-RAY DIFFRACTION (3.1 Å)