7QQP

SpCas9 bound to AAVS1 off-target3 DNA substrate

Summary for 7QQP

Entry DOI	10.2210/pdb7qqp/pdb
Related	7OX9
Descriptor	AAVS1 sgRNA, CRISPR-associated endonuclease Cas9/Csn1, AAVS1 off-target3 target strand, ... (8 entities in total)
Functional Keywords	crispr, cas9, off-target, ternary complex, hydrolase
Biological source	Streptococcus pyogenes More
Total number of polymer chains	4
Total formula weight	198657.77
Authors	Pacesa, M.,JInek, M. (deposition date: 2022-01-10, release date: 2022-10-26, Last modification date: 2024-01-31)
Primary citation	Pacesa, M.,Lin, C.H.,Clery, A.,Saha, A.,Arantes, P.R.,Bargsten, K.,Irby, M.J.,Allain, F.H.,Palermo, G.,Cameron, P.,Donohoue, P.D.,Jinek, M. Structural basis for Cas9 off-target activity. Cell, 185:4067-4081.e21, 2022 Cited by PubMed: 36306733 DOI: 10.1016/j.cell.2022.09.026 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.6 Å)

Structure validation