7OX7

Target-bound SpCas9 complex with TRAC chimeric RNA-DNA guide

Summary for 7OX7

Entry DOI	10.2210/pdb7ox7/pdb
Descriptor	chimeric RNA-DNA guide, CRISPR-associated endonuclease Cas9/Csn1, TRAC target DNA strand, ... (7 entities in total)
Functional Keywords	cas9, crispr, chrdna, trac, nuclease, hydrolase
Biological source	Streptococcus pyogenes serotype M1 More
Total number of polymer chains	4
Total formula weight	197718.59
Authors	Donohoue, P.,Pacesa, M.,Lau, E.,Vidal, B.,Irby, M.J.,Nyer, D.B.,Rotstein, T.,Banh, L.,Toh, M.T.,Gibson, J.,Kohrs, B.,Baek, K.,Owen, A.L.G.,Slorach, E.M.,van Overbeek, M.,Fuller, C.K.,May, A.P.,Jinek, M.,Cameron, P. (deposition date: 2021-06-22, release date: 2021-09-15, Last modification date: 2024-01-31)
Primary citation	Donohoue, P.D.,Pacesa, M.,Lau, E.,Vidal, B.,Irby, M.J.,Nyer, D.B.,Rotstein, T.,Banh, L.,Toh, M.S.,Gibson, J.,Kohrs, B.,Baek, K.,Owen, A.L.G.,Slorach, E.M.,van Overbeek, M.,Fuller, C.K.,May, A.P.,Jinek, M.,Cameron, P. Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells. Mol.Cell, 81:3637-3649.e5, 2021 Cited by PubMed: 34478654 DOI: 10.1016/j.molcel.2021.07.035 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.6 Å)

Structure validation