7C7L

Cryo-EM structure of the Cas12f1-sgRNA-target DNA complex

Summary for 7C7L

• Entry DOI: 10.2210/pdb7c7l/pdb
• EMDB information: 30299
• Descriptor: CRISPR-associated protein Cas14a.1, sgRNA, DNA (40-mer), ... (5 entities in total)
• Functional Keywords: cas12f, cas14, sgrna, target dna, crispr, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• Biological source: uncultured archaeon; More
• Total number of polymer chains: 5
• Total formula weight: 208448.84

Authors

Takeda, N.S.,Nakagawa, R.,Okazaki, S.,Hirano, H.,Kobayashi, K.,Kusakizako, T.,Nishizawa, T.,Yamashita, K.,Nishimasu, H.,Nureki, O. (deposition date: 2020-05-26, release date: 2020-12-23, Last modification date: 2024-03-27)

• Primary citation: Takeda, S.N.,Nakagawa, R.,Okazaki, S.,Hirano, H.,Kobayashi, K.,Kusakizako, T.,Nishizawa, T.,Yamashita, K.,Nishimasu, H.,Nureki, O.; Structure of the miniature type V-F CRISPR-Cas effector enzyme.; Mol.Cell, 81:558-570.e3, 2021; PubMed: 33333018; DOI: 10.1016/j.molcel.2020.11.035

Experimental method

ELECTRON MICROSCOPY (3.3 Å)