6VO8

X-ray structure of the Cj1427 in the presence of NADH and GDP-D-glycero-D-mannoheptose, an essential NAD-dependent dehydrogenase from Campylobacter jejuni

Summary for 6VO8

• Entry DOI: 10.2210/pdb6vo8/pdb
• Related: 6vo6
• Descriptor: Putative sugar-nucleotide epimerase/dehydratease, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, [[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{S},5~{S},6~{S})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl] hydrogen phosphate, ... (4 entities in total)
• Functional Keywords: capsular polysaccharide, oxidoreductase, dehydrogenase, isomerase
• Biological source: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
• Total number of polymer chains: 2
• Total formula weight: 76040.18

Authors

Spencer, K.D.,Anderson, T.K.,Thoden, J.B.,Huddleston, J.P.,Raushel, F.M.,Holden, H.M. (deposition date: 2020-01-30, release date: 2020-03-25, Last modification date: 2023-10-11)

• Primary citation: Huddleston, J.P.,Anderson, T.K.,Spencer, K.D.,Thoden, J.B.,Raushel, F.M.,Holden, H.M.; Structural Analysis of Cj1427, an Essential NAD-Dependent Dehydrogenase for the Biosynthesis of the Heptose Residues in the Capsular Polysaccharides ofCampylobacter jejuni.; Biochemistry, 59:1314-1327, 2020; PubMed: 32168450; DOI: 10.1021/acs.biochem.0c00096

Experimental method

X-RAY DIFFRACTION (2.4 Å)