6O70

Crystal structure of Csm6 H132A mutant in complex with cA4 by cocrystallization of cA4 and Csm6 H132A mutant

Summary for 6O70

• Entry DOI: 10.2210/pdb6o70/pdb
• Descriptor: Csm6, 2',3'- cyclic AMP, 3'-O-[(R)-{[(2S,3aS,4S,6S,6aS)-6-(6-amino-9H-purin-9-yl)-2-hydroxy-2-oxotetrahydro-2H-2lambda~5~-furo[3,4-d][1,3,2]dioxaphosphol-4-yl]methoxy}(hydroxy)phosphoryl]adenosine, ... (4 entities in total)
• Functional Keywords: type iii-a crispr-cas system; csm6 h132a mutant in complex with ca4, immune system
• Biological source: Thermococcus onnurineus (strain NA1)
• Total number of polymer chains: 2
• Total formula weight: 101431.33

Authors

Jia, N.,Patel, D.J. (deposition date: 2019-03-07, release date: 2019-07-31, Last modification date: 2023-10-11)

• Primary citation: Jia, N.,Jones, R.,Yang, G.,Ouerfelli, O.,Patel, D.J.; CRISPR-Cas III-A Csm6 CARF Domain Is a Ring Nuclease Triggering Stepwise cA4Cleavage with ApA>p Formation Terminating RNase Activity.; Mol.Cell, 75:944-956.e6, 2019; PubMed: 31326273; DOI: 10.1016/j.molcel.2019.06.014

Experimental method

X-RAY DIFFRACTION (2.3 Å)