6DOX
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 360 s at 21 C
Summary for 6DOX
Entry DOI | 10.2210/pdb6dox/pdb |
Group deposition | Cleavage of RNA by B. Halodurans Ribonuclease H1 (G_1001001) |
Descriptor | Ribonuclease H, DI(HYDROXYETHYL)ETHER, 5'-R(*AP*CP*AP*U)-3' portion of cleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3', ... (11 entities in total) |
Functional Keywords | protein-rna-dna complex, double helix, rna hydrolysis, in crystallo catalysis, metal dependent catalysis, monovalent cations, divalent cations, hydrolase, hydrolase-rna-dna complex, hydrolase/rna/dna |
Biological source | Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) More |
Total number of polymer chains | 4 |
Total formula weight | 20892.50 |
Authors | Samara, N.L.,Yang, W. (deposition date: 2018-06-09, release date: 2018-08-15, Last modification date: 2023-10-11) |
Primary citation | Samara, N.L.,Yang, W. Cation trafficking propels RNA hydrolysis. Nat. Struct. Mol. Biol., 25:715-721, 2018 Cited by PubMed: 30076410DOI: 10.1038/s41594-018-0099-4 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.45 Å) |
Structure validation
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