5KNL

Crystal structure of S. pombe ubiquitin E1 (Uba1) in complex with Ubc15 and ubiquitin

Summary for 5KNL

• Entry DOI: 10.2210/pdb5knl/pdb
• Descriptor: Ubiquitin-activating enzyme E1 1, Ubiquitin, Ubiquitin-conjugating enzyme E2 15, ... (5 entities in total)
• Functional Keywords: ubiquitin, e1, e2, uba1, ubc15, conformational change, thioester, adenylation, thioester transfer (transthioesterification), atp-binding, ubiquitin e2 binding, ubiquitination, ligase
• Biological source: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); More
• Total number of polymer chains: 6
• Total formula weight: 286600.94

Authors

Olsen, S.K.,Lv, Z.,Yuan, L.,Williams, K. (deposition date: 2016-06-28, release date: 2017-02-15, Last modification date: 2023-09-27)

• Primary citation: Lv, Z.,Rickman, K.A.,Yuan, L.,Williams, K.,Selvam, S.P.,Woosley, A.N.,Howe, P.H.,Ogretmen, B.,Smogorzewska, A.,Olsen, S.K.; S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity.; Mol. Cell, 65:699-714.e6, 2017; PubMed: 28162934; DOI: 10.1016/j.molcel.2017.01.008

Experimental method

X-RAY DIFFRACTION (2.5 Å)