5J7K

Loop grafting onto a highly stable FN3 scaffold

Summary for 5J7K

• Entry DOI: 10.2210/pdb5j7k/pdb
• Related: 5J7C
• Descriptor: FN3con-a-lys, ZINC ION (3 entities in total)
• Functional Keywords: loop grafting, rational design, fibronectin type iii, fn3, protein binding, protein binding-hydrolase complex
• Biological source: synthetic construct
• Total number of polymer chains: 8
• Total formula weight: 89075.76

Authors

Porebski, B.T.,Drinkwater, N.,McGowan, S.,Buckle, A.M. (deposition date: 2016-04-06, release date: 2016-08-17, Last modification date: 2023-09-27)

• Primary citation: Porebski, B.T.,Conroy, P.J.,Drinkwater, N.,Schofield, P.,Vazquez-Lombardi, R.,Hunter, M.R.,Hoke, D.E.,Christ, D.,McGowan, S.,Buckle, A.M.; Circumventing the stability-function trade-off in an engineered FN3 domain.; Protein Eng.Des.Sel., 2016; PubMed: 27578887; DOI: 10.1093/protein/gzw046

Experimental method

X-RAY DIFFRACTION (2.46 Å)