5F9R
Crystal structure of catalytically-active Streptococcus pyogenes CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA primed for target DNA cleavage
Summary for 5F9R
Entry DOI | 10.2210/pdb5f9r/pdb |
Descriptor | CRISPR-associated endonuclease Cas9/Csn1, RNA (116-MER), DNA (30-MER), ... (5 entities in total) |
Functional Keywords | crispr, cas9, r-loop, genome engineering, hydrolase-dna-rna complex, hydrolase/dna/rna |
Biological source | Streptococcus pyogenes serotype M1 More |
Total number of polymer chains | 4 |
Total formula weight | 215728.56 |
Authors | Jiang, F.,Doudna, J.A. (deposition date: 2015-12-10, release date: 2016-01-27, Last modification date: 2024-03-06) |
Primary citation | Jiang, F.,Taylor, D.W.,Chen, J.S.,Kornfeld, J.E.,Zhou, K.,Thompson, A.J.,Nogales, E.,Doudna, J.A. Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage. Science, 351:867-871, 2016 Cited by PubMed: 26841432DOI: 10.1126/science.aad8282 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.4 Å) |
Structure validation
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