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4KD8

70S Ribosome translocation intermediate FA-3.6A CONTAINING ELONGATION FACTOR EFG/FUSIDIC ACID/GDP, MRNA, AND TRNA BOUND IN THE pe*/E STATE. THIS ENTRY CONTAINS THE 30S RIBOSOMAL SUBUNIT A. THE 50S SUBUNIT A CAN BE FOUND IN 4KD9. MOLECULE B IN THE SAME ASYMMETRIC UNIT IS DEPOSITED AS 4KDA (30S) AND 4KDB (50S).

Summary for 4KD8

Split4KD9 4KDA 4KDB 
Combined entry4V9L
Related PRD IDPRD_000226 
Descriptor30S ribosomal protein S2, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14 type Z, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S17, 30S ribosomal protein S18, 30S ribosomal protein S19, 30S ribosomal protein S20, Elongation factor G
Functional KeywordsFUSIDIC ACID GDP, CHIMERIC HYBRID STATE RIBOSOME, RIBOSOME, TRANSLOCATION INTERMEDIATE, EF-G, MRNA, TRNA, RIBOSOME-ANTIBIOTIC complex,RIBOSOME/ANTIBIOTIC
Biological sourceThermus thermophilus
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Cellular locationCytoplasm (By similarity) 
Total number of polymer chains24
Total molecular weight871488.32
Authors
Zhou, J.,Lancaster, L.,Donohue, J.P.,Noller, H.F. (deposition date: 2013-04-24, release date: 2013-07-03, modification date: 2013-08-07)
Primary citation
Zhou, J.,Lancaster, L.,Donohue, J.P.,Noller, H.F.
Crystal structures of EF-G-ribosome complexes trapped in intermediate states of translocation.
Science, 340:1236086-1236086, 2013
PubMed: 23812722
DOI: 10.1126/science.1236086
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.5 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone2310.2%16.8%0.44MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolutionDownload full validation report

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entries available on 2014-10-22
00:00 UTC / 09:00 JST