4V9L

70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state.

This is a large structure.

Summary for 4V9L

Integrates4KD8 4KD9 4KDA 4KDB
Related PRD IDPRD_000226
DescriptorRibosome
Functional KeywordsFUSIDIC ACID GDP, CHIMERIC HYBRID STATE RIBOSOME, RIBOSOME, TRANSLOCATION INTERMEDIATE, EF-G, MRNA, TRNA, RIBOSOME-ANTIBIOTIC complex,RIBOSOME/ANTIBIOTIC
Biological sourceThermus thermophilus
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Cellular locationCytoplasm  Q72I01
Total number of polymer chains122
Total molecular weight4570580.17
Authors
Zhou, J.,Lancaster, L.,Donohue, J.P.,Noller, H.F. (deposition date: 2013-04-24, release date: 2014-07-09, modification date: 2014-12-10)
Primary citation
Zhou, J.,Lancaster, L.,Donohue, J.P.,Noller, H.F.
Crystal structures of EF-G-ribosome complexes trapped in intermediate states of translocation.
Science, 340:1236086-1236086, 2013
PubMed: 23812722
DOI: 10.1126/science.1236086
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.5 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.3422411.6%18.2%5.7%0.44MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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rotated about x axis by 90°
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