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4KBW

70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. This entry contains 50S ribosomal subunit B. The full asymmetric unit also contains PDB entries 4KBV (30S subunit B), 4KBT (30S subunit A), and 4KBU (50S subunit A).

Summary for 4KBW

Split4KBT 4KBU 4KBV 
Combined entry4V9J
Descriptor50S ribosomal protein L1, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, unknown protein, 50S ribosomal protein L11, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L31, 50S ribosomal protein L13, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, 50S ribosomal protein L7/L12
Functional KeywordsGDPNP, chimeric hybrid state ribosome, translocation intermediate, EF-G, mRNA, tRNA, RIBOSOME-ANTIBIOTIC complex,RIBOSOME/ANTIBIOTIC
Biological sourceThermus thermophilus
Total number of polymer chains37
Total molecular weight1413896.73
Authors
Zhou, J.,Lancaster, L.,Donohue, J.P.,Noller, H.F. (deposition date: 2013-04-23, release date: 2013-07-03, modification date: 2013-07-17)
Primary citation
Zhou, J.,Lancaster, L.,Donohue, J.P.,Noller, H.F.
Crystal structures of EF-G-ribosome complexes trapped in intermediate states of translocation.
Science, 340:1236086-1236086, 2013
PubMed: 23812722
DOI: 10.1126/science.1236086
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.86 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.3332512.6%20.0%13.2%0.42MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolutionDownload full validation report

More Asymmetric unit images

no rotation
rotated about x axis by 90°
rotated about y axis by 90°
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entries available on 2014-10-29
00:00 UTC / 09:00 JST