4AQX

Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of magnesium

Summary for 4AQX

• Entry DOI: 10.2210/pdb4aqx/pdb
• Related: 1AF5 1BP7 1G9Y 1G9Z 1MOW 1N3E 1N3F 1T9I 1T9J 1U0C 1U0D 2VBJ 2VBL 2VBN 2VBO 4AAB 4AAD 4AAE 4AAF 4AAG 4AQU
• Descriptor: DNA ENDONUCLEASE I-CREI, 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP)-3', 5'-D(*GP*AP*CP*AP*GP*TP*TP*TP*GP*GP)-3', ... (8 entities in total)
• Functional Keywords: hydrolase, methylation, gene targeting, genetics, protein-dna interaction, homing endonucleases
• Biological source: CHLAMYDOMONAS REINHARDTII; More
• Cellular location: Plastid, chloroplast: P05725
• Total number of polymer chains: 6
• Total formula weight: 50228.28

Authors

Valton, J.,Daboussi, F.,Leduc, S.,Redondo, P.,Macmaster, R.,Molina, R.,Montoya, G.,Duchateau, P. (deposition date: 2012-04-19, release date: 2012-07-04, Last modification date: 2023-12-20)

• Primary citation: Valton, J.,Daboussi, F.,Leduc, S.,Molina, R.,Redondo, P.,Macmaster, R.,Montoya, G.,Duchateau, P.; 5'-Cytosine-Phosphoguanine (Cpg) Methylation Impacts the Activity of Natural and Engineered Meganucleases.; J.Biol.Chem., 287:30139-, 2012; PubMed: 22740697; DOI: 10.1074/JBC.M112.379966

Experimental method

X-RAY DIFFRACTION (2.2 Å)