3HT3

Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus V713P mutant bound to G:dCTP

Summary for 3HT3

• Entry DOI: 10.2210/pdb3ht3/pdb
• Related: 3HP6 3HPO
• Related PRD ID: PRD_900003
• Descriptor: DNA polymerase I, large fragment, 5'-D(*CP*GP*AP*TP*CP*AP*CP*GP*(DOC))-3', 5'-D(*AP*CP*GP*GP*CP*GP*TP*GP*AP*TP*CP*G)-3', ... (8 entities in total)
• Functional Keywords: protein-dna complex, dna polymerase i, dna replication, dna-binding, dna-directed dna polymerase, hydrolase, nuclease, nucleotidyltransferase, transferase, transferase-dna complex, transferase/dna
• Biological source: Geobacillus stearothermophilus; More
• Total number of polymer chains: 6
• Total formula weight: 147653.04

Authors

Wu, E.Y.,Beese, L.S. (deposition date: 2009-06-11, release date: 2010-07-14, Last modification date: 2023-09-06)

• Primary citation: Wu, E.Y.,Beese, L.S.; The structure of a high fidelity DNA polymerase bound to a mismatched nucleotide reveals an "ajar" intermediate conformation in the nucleotide selection mechanism.; J.Biol.Chem., 286:19758-19767, 2011; PubMed: 21454515; DOI: 10.1074/jbc.M110.191130

Experimental method

X-RAY DIFFRACTION (1.7 Å)