3C0W

I-SceI in complex with a bottom nicked DNA substrate

Summary for 3C0W

• Entry DOI: 10.2210/pdb3c0w/pdb
• Related: 1R7M 3COX
• Descriptor: DNA (5'-D(*DAP*DCP*DGP*DCP*DTP*DAP*DGP*DGP*DGP*DAP*DTP*DAP*DAP*DCP*DAP*DGP*DGP*DGP*DTP*DAP*DAP*DTP*DAP*DC)-3'), DNA (5'-D(*DGP*DTP*DAP*DTP*DTP*DAP*DCP*DCP*DCP*DTP*DGP*DTP*DTP*DAP*DT)-3'), DNA (5'-D(P*DCP*DCP*DCP*DTP*DAP*DGP*DCP*DGP*DT)-3'), ... (7 entities in total)
• Functional Keywords: endonuclease, homing, ladlidadg, catalytic mechanism, metal binding, nicked intermediate, hydrolase, intron homing, mitochondrion, mrna processing, mrna splicing, hydrolase-dna complex, hydrolase/dna
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Cellular location: Mitochondrion: P03882
• Total number of polymer chains: 4
• Total formula weight: 42554.03

Authors

Moure, C.M.,Gimble, F.S.,Quiocho, F.A. (deposition date: 2008-01-21, release date: 2008-05-06, Last modification date: 2023-08-30)

• Primary citation: Moure, C.M.,Gimble, F.S.,Quiocho, F.A.; Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway.; Nucleic Acids Res., 36:3287-3296, 2008; PubMed: 18424798; DOI: 10.1093/nar/gkn178

Experimental method

X-RAY DIFFRACTION (2.2 Å)