2LV0

Solution Structure of Helix-35 Stem-loop from E. coli 23S rRNA

> Summary

Summary for 2LV0

Descriptor5'-R(*GP*GP*GP*CP*UP*AP*AP*UP*GP*UP*UP*GP*AP*AP*AP*AP*AP*UP*UP*AP*GP*CP*CP*C)-3'
Functional Keywordsribosome, 23s rrna, methylation, erythromycin resistance, rna
Total number of polymer chains1
Total molecular weight7717.7
Authors
Nikonowicz, E.P.,Wang, J.,Moran, S.,Donarski, J. (deposition date: 2012-06-26, release date: 2013-06-26)
Primary citation
Moran, S.,Donarski, J.,Wang, J.,Nikonowicz, E.P.
Solution Structure of Helix-35 Stem-loop from E. coli 23S rRNA
To be Published,
Experimental method
SOLUTION NMR
NMR Information
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Structure validation

ClashscoreRNA backbone80.42MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all NMR structures

More Asymmetric unit images

Molmil generated image of 2lv0
no rotation
Molmil generated image of 2lv0
rotated about x axis by 90°
Molmil generated image of 2lv0
rotated about y axis by 90°

> Structural details

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
ARNA (5'-R(*GP*GP*GP*CP*UP*AP*AP*UP*GP*UP*UP*GP*AP*AP*AP*AP*AP*UP*UP*AP*GP*CP*CP*C)-3')polymer247717.71

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains1
Total molecular weight7717.7
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight7717.7
*Water molecules are not included.

> Experimental details

Spectrometer

Experimental method:SOLUTION NMR
Spectrometer IDSpectrometer makerSpectrometer modelSpectrometer typeSpectrometer field strength
1VarianINOVAVarian INOVA600
2VarianINOVAVarian INOVA500

Experiment

experiment idconditions idsolution idExperiment type
1242D 1H-15N HSQC
2112D 1H-13C HSQC
3113D HCCH-TOCSY
4113D 1H-13C NOESY
5122D 1H-1H NOESY
6122D DQF-COSY
7232D 1H-1H NOESY
8113D HCCH-COSY
9122D 1H-31P HetCor
10112D 1H-15N HMQC
11112D 1H-13C ctHSQC-31P
12112D 1H-15N HCN
13243D 1H-15N NOESY

NMR Sample

conditions idNMR sample pHNMR sample pressureNMR sample temperature
16.8ambient298
26.8ambient283

Conformers

Conformers Calculated Total Number100
Conformers Submitted Total Number11

> Functional details

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Functional Information from GO Data

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Functional Information from PDB Data

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Functional Information from PDB atom coordinates for the "HETATM" binding sites

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Functional Information from PROSITE/UniProt

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Functional Information from SwissProt/UniProt

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Catalytic Information from CSA

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Catalytic Information from CATRES

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> Sequence Neighbor

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