2GIH

Q138F HincII bound to cognate DNA GTCGAC and Ca2+

Summary for 2GIH

• Entry DOI: 10.2210/pdb2gih/pdb
• Related: 1TX3 2GIE 2GIG 2GII 2GIJ
• Descriptor: 5'-D(*GP*CP*CP*GP*GP*TP*CP*GP*AP*CP*CP*GP*GP*C)-3', Type II restriction enzyme HincII, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: protein dna complex, indirect readout, dna intercalation, endonuclease, hydrolase-dna complex, hydrolase/dna
• Biological source: Haemophilus influenzae
• Total number of polymer chains: 4
• Total formula weight: 68323.91

Authors

Horton, N.C.,Joshi, H.K.,Etzkorn, C.,Chatwell, L.,Bitinaite, J. (deposition date: 2006-03-28, release date: 2006-07-18, Last modification date: 2024-02-14)

• Primary citation: Joshi, H.K.,Etzkorn, C.,Chatwell, L.,Bitinaite, J.,Horton, N.C.; Alteration of Sequence Specificity of the Type II Restriction Endonuclease HincII through an Indirect Readout Mechanism.; J.Biol.Chem., 281:23852-23869, 2006; PubMed: 16675462; DOI: 10.1074/jbc.M512339200

Experimental method

X-RAY DIFFRACTION (2.5 Å)