2EZU

Pyruvate oxidase variant F479W in complex with reaction intermediate 2-acetyl-thiamin diphosphate

Summary for 2EZU

• Entry DOI: 10.2210/pdb2ezu/pdb
• Related: 1POW 1POX 1Y9D 2EZ4 2EZ8 2EZ9 2EZT
• Descriptor: Pyruvate oxidase, MAGNESIUM ION, SODIUM ION, ... (7 entities in total)
• Functional Keywords: tpp enzyme, reaction intermediate, oxidoreductase
• Biological source: Lactobacillus plantarum
• Total number of polymer chains: 2
• Total formula weight: 135268.19

Authors

Wille, G.,Meyer, D.,Steinmetz, A.,Hinze, E.,Golbik, R.,Tittmann, K. (deposition date: 2005-11-10, release date: 2006-04-25, Last modification date: 2023-11-15)

• Primary citation: Wille, G.,Meyer, D.,Steinmetz, A.,Hinze, E.,Golbik, R.,Tittmann, K.; The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography.; Nat.Chem.Biol., 2:324-328, 2006; PubMed: 16680160; DOI: 10.1038/nchembio788

Experimental method

X-RAY DIFFRACTION (2.16 Å)