1Z7C

Crystal Structure of Human Placental Lactogen

> Summary

Summary for 1Z7C

DescriptorChorionic somatomammotropin hormone
Functional Keywordsfour-helix bundle protein, hormone-growth factor complex, hormone/growth factor
Biological sourceHomo sapiens (human)
Cellular locationSecreted P01243
Total number of polymer chains1
Total molecular weight22335.3
Authors
Walsh, S.T.R.,Kossiakoff, A.A. (deposition date: 2005-03-24, release date: 2006-03-07, modification date: 2011-07-13)
Primary citation
Walsh, S.T.R.,Kossiakoff, A.A.
Crystal Structure and Site 1 Binding Energetics of Human Placental Lactogen.
J.Mol.Biol., 358:773-784, 2006
PubMed: 16546209
DOI: 10.1016/j.jmb.2006.02.038
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.268253.3%14.8%8.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 1z7c
no rotation
Molmil generated image of 1z7c
rotated about x axis by 90°
Molmil generated image of 1z7c
rotated about y axis by 90°

> Structural details

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
AChorionic somatomammotropin hormonepolymer19122335.31
UniProt (P01243)
Pfam (PF00103)
UniProt (by SIFTS) (P0DML2)
Homo sapiens (human)@PDBjChoriomammotropin, Lactogen
waterwater18.065

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains1
Total molecular weight22335.3
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight22335.3
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (2 Å)

Cell axes115.50430.04753.490
Cell angles90.00113.3890.00
SpacegroupC 1 2 1
Resolution limits15.00 - 2.00
the highest resolution shell value2.051 - 2.000
R-factor0.23308
R-work0.22939
the highest resolution shell value0.286
R-free0.26704
the highest resolution shell value0.362
RMSD bond length0.023
RMSD bond angle2.141

Data Collection Statistics

Resolution limits30.00 - 1.90
the highest resolution shell value -
Number of reflections13322
Rmerge_l_obs0.050
the highest resolution shell value0.505
Completeness95.9
Redundancy3.6
the highest resolution shell value2
I/sigma(I)2

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, SITTING DROP6.5292
1VAPOR DIFFUSION, SITTING DROP6.5292
1VAPOR DIFFUSION, SITTING DROP6.5292

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*

> Functional details

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Functional Information from GO Data

ChainGOidnamespacecontents
A0005783cellular_componentendoplasmic reticulum
A0005576cellular_componentextracellular region
A0031988cellular_componentmembrane-bounded vesicle
A0046872molecular_functionmetal ion binding
A0060397biological_processJAK-STAT cascade involved in growth hormone signaling pathway
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Functional Information from PDB Data

site_idNumber of ResiduesDetails
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Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
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Functional Information from PROSITE/UniProt

site_idNumber of ResiduesDetails
PS003381Somatotropin, prolactin and related hormones signature 2. C-[LIVMFY]-{PT}-x-D-[LIVMFYSTA]-x-{S}-{RK}-{A}-x-[LIVMFY]-x(2)-[LIVMFYT]
ChainResidueDetails
ANA*

PS002661Somatotropin, prolactin and related hormones signature 1. C-x-[STN]-x(2)-[LIVMFYS]-x-[LIVMSTA]-P-x(5)-[TALIV]-x(7)-[LIVMFY]-x(6)
ChainResidueDetails
ANA*

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Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails
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Catalytic Information from CSA

site_idNumber of ResiduesDetails
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Catalytic Information from CATRES

site_idNumber of ResiduesDetails

> Sequence Neighbor

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