1HUM

SOLUTION STRUCTURE OF THE CHEMOKINE HMIP-1BETA(SLASH)ACT-2 BY MULTI-DIMENSIONAL NMR: A NOVEL CHEMOKINE DIMER

> Summary

Summary for 1HUM

DescriptorHUMAN MACROPHAGE INFLAMMATORY PROTEIN 1 BETA (HMIP-1B) (NMR, MINIMIZED AVERAGE STRUCTURE)
Functional Keywordscytokine(chemotactic)
Biological sourceHomo sapiens (human)
Total number of polymer chains2
Total molecular weight15649.6
Authors
Clore, G.M.,Lodi, P.J.,Garrett, D.S.,Gronenborn, A.M. (deposition date: 1994-01-31, release date: 1994-04-30, modification date: 2009-02-24)
Primary citation
Lodi, P.J.,Garrett, D.S.,Kuszewski, J.,Tsang, M.L.,Weatherbee, J.A.,Leonard, W.J.,Gronenborn, A.M.,Clore, G.M.
High-resolution solution structure of the beta chemokine hMIP-1 beta by multidimensional NMR.
Science, 263:1762-1767, 1994
PubMed: 8134838
MImport into Mendeley
Experimental method
SOLUTION NMR
NMR Information
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Structure validation

ClashscoreRamachandran outliersSidechain outliers127.5%11.1%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all NMR structures

More Asymmetric unit images

Molmil generated image of 1hum
no rotation
Molmil generated image of 1hum
rotated about x axis by 90°
Molmil generated image of 1hum
rotated about y axis by 90°

> Structural details

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
A, BHUMAN MACROPHAGE INFLAMMATORY PROTEIN 1 BETApolymer697824.82
UniProt (P13236)
Pfam (PF00048)
Homo sapiens (human)@PDBj
waterwater18.04

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains2
Total molecular weight15649.6
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight15649.6
*Water molecules are not included.

> Experimental details

Spectrometer

Experimental method:SOLUTION NMR

Spectrometer IDSpectrometer makerSpectrometer modelSpectrometer typeSpectrometer field strength

Experiment

experiment idconditions idsolution idExperiment type

NMR Sample

conditions idNMR sample pHNMR sample pressureNMR sample temperature

Conformers

Conformers Calculated Total Number
Conformers Submitted Total Number1

> Functional details

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Functional Information from GO Data

ChainGOidnamespacecontents
A0005576cellular_componentextracellular region
A0005615cellular_componentextracellular space
A0031726molecular_functionCCR1 chemokine receptor binding
A0031730molecular_functionCCR5 chemokine receptor binding
A0008009molecular_functionchemokine activity
A0005125molecular_functioncytokine activity
A0042802molecular_functionidentical protein binding
A0007155biological_processcell adhesion
A0007267biological_processcell-cell signaling
A0071346biological_processcellular response to interferon-gamma
A0071347biological_processcellular response to interleukin-1
A0071356biological_processcellular response to tumor necrosis factor
A0070098biological_processchemokine-mediated signaling pathway
A0007163biological_processestablishment or maintenance of cell polarity
A0007186biological_processG-protein coupled receptor signaling pathway
A0006955biological_processimmune response
A0006954biological_processinflammatory response
A0002548biological_processmonocyte chemotaxis
A0006928biological_processmovement of cell or subcellular component
A0043922biological_processnegative regulation by host of viral transcription
A0030593biological_processneutrophil chemotaxis
A0051928biological_processpositive regulation of calcium ion transport
A0050850biological_processpositive regulation of calcium-mediated signaling
A0070374biological_processpositive regulation of ERK1 and ERK2 cascade
A0043547biological_processpositive regulation of GTPase activity
A0050729biological_processpositive regulation of inflammatory response
A2000503biological_processpositive regulation of natural killer cell chemotaxis
A0009636biological_processresponse to toxic substance
A0009615biological_processresponse to virus
A0007165biological_processsignal transduction
B0005576cellular_componentextracellular region
B0005615cellular_componentextracellular space
B0031726molecular_functionCCR1 chemokine receptor binding
B0031730molecular_functionCCR5 chemokine receptor binding
B0008009molecular_functionchemokine activity
B0005125molecular_functioncytokine activity
B0042802molecular_functionidentical protein binding
B0007155biological_processcell adhesion
B0007267biological_processcell-cell signaling
B0071346biological_processcellular response to interferon-gamma
B0071347biological_processcellular response to interleukin-1
B0071356biological_processcellular response to tumor necrosis factor
B0070098biological_processchemokine-mediated signaling pathway
B0007163biological_processestablishment or maintenance of cell polarity
B0007186biological_processG-protein coupled receptor signaling pathway
B0006955biological_processimmune response
B0006954biological_processinflammatory response
B0002548biological_processmonocyte chemotaxis
B0006928biological_processmovement of cell or subcellular component
B0043922biological_processnegative regulation by host of viral transcription
B0030593biological_processneutrophil chemotaxis
B0051928biological_processpositive regulation of calcium ion transport
B0050850biological_processpositive regulation of calcium-mediated signaling
B0070374biological_processpositive regulation of ERK1 and ERK2 cascade
B0043547biological_processpositive regulation of GTPase activity
B0050729biological_processpositive regulation of inflammatory response
B2000503biological_processpositive regulation of natural killer cell chemotaxis
B0009636biological_processresponse to toxic substance
B0009615biological_processresponse to virus
B0007165biological_processsignal transduction
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Functional Information from PDB Data

site_idNumber of ResiduesDetails
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Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
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Functional Information from PROSITE/UniProt

site_idNumber of ResiduesDetails
PS004722Small cytokines (intercrine/chemokine) C-C subfamily signature. C-C-[LIFYTRQ]-x(5,8)-[LIR]-x(4)-[LIVMFA]-x(2)-[FYWECI]-x(5,8)-C-x(3,4)
ChainResidueDetails
ANA*
BNA*

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Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails
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Catalytic Information from CSA

site_idNumber of ResiduesDetails
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Catalytic Information from CATRES

site_idNumber of ResiduesDetails

> Sequence Neighbor

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