1DL6

SOLUTION STRUCTURE OF HUMAN TFIIB N-TERMINAL DOMAIN

Summary for 1DL6

• Entry DOI: 10.2210/pdb1dl6/pdb
• Descriptor: TRANSCRIPTION FACTOR II B (TFIIB), ZINC ION (2 entities in total)
• Functional Keywords: zinc ribbon, gene regulation
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: Q00403
• Total number of polymer chains: 1
• Total formula weight: 6434.55

Authors

Chen, H.-T.,Legault, P.,Glushka, J.,Omichinski, J.G.,Scott, R.A. (deposition date: 1999-12-08, release date: 2000-10-18, Last modification date: 2024-05-22)

Primary citation

Chen, H.T.,Legault, P.,Glushka, J.,Omichinski, J.G.,Scott, R.A.
Structure of a (Cys3His) zinc ribbon, a ubiquitous motif in archaeal and eucaryal transcription.
Protein Sci., 9:1743-1752, 2000

PubMed Abstract: Transcription factor IIB (TFIIB) is an essential component in the formation of the transcription initiation complex in eucaryal and archaeal transcription. TFIIB interacts with a promoter complex containing the TATA-binding protein (TBP) to facilitate interaction with RNA polymerase II (RNA pol II) and the associated transcription factor IIF (TFIIF). TFIIB contains a zinc-binding motif near the N-terminus that is directly involved in the interaction with RNA pol II/TFIIF and plays a crucial role in selecting the transcription initiation site. The solution structure of the N-terminal residues 2-59 of human TFIIB was determined by multidimensional NMR spectroscopy. The structure consists of a nearly tetrahedral Zn(Cys)3(His)1 site confined by type I and "rubredoxin" turns, three antiparallel beta-strands, and disordered loops. The structure is similar to the reported zinc-ribbon motifs in several transcription-related proteins from archaea and eucarya, including Pyrococcus furiosus transcription factor B (PfTFB), human and yeast transcription factor IIS (TFIIS), and Thermococcus celer RNA polymerase II subunit M (TcRPOM). The zinc-ribbon structure of TFIIB, in conjunction with the biochemical analyses, suggests that residues on the beta-sheet are involved in the interaction with RNA pol II/TFIIF, while the zinc-binding site may increase the stability of the beta-sheet.

PubMed: 11045620

Experimental method

SOLUTION NMR