8V7L
Cryo-EM structure of singly-bound SNF2h-nucleosome complex with SNF2h at inactive SHL2 (conformation 2)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, E | Histone H3.2 | polymer | 135 | 15271.9 | 2 | UniProt (P84233) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | |
2 | B, F | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62799) Pfam (PF15511) In PDB | Xenopus laevis (African clawed frog) | |
3 | C, G | Histone H2A type 1 | polymer | 129 | 13978.2 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) In PDB | Xenopus laevis (African clawed frog) | |
4 | D, H | Histone H2B | polymer | 122 | 13524.8 | 2 | UniProt (P02281) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | H2B1.1 |
5 | I | Widom 601 DNA (147-mer) plus 60 base pairs flanking DNA (reverse strand) | polymer | 207 | 64200.9 | 1 | synthetic construct | ||
6 | J | Widom 601 DNA (147-mer) with 60 base pairs flanking DNA (forward strand) | polymer | 207 | 63626.5 | 1 | synthetic construct | ||
7 | W | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 | polymer | 1052 | 122089.3 | 1 | UniProt (O60264) Pfam (PF00176) Pfam (PF00271) Pfam (PF09110) Pfam (PF09111) In PDB | Homo sapiens (human) | |
8 | W | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
9 | W | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A, E: 1 - 135 (UniProt: P84233)
C, G: 1 - 129 (UniProt: P06897)
D, H: 1 - 122 (UniProt: P02281)
PDB | External Database | Details |
---|---|---|
Ala 102 | Gly 103 | engineered mutation |
Ala 110 | Cys 111 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Arg 99 | Gly 100 | engineered mutation |
Ser 123 | Ala 124 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Thr 29 | Ser 33 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 11 |
Total formula weight | 357992.9 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 451.5 | |
All* | Total formula weight | 358444.4 |