8V06

Crystal structure of mouse PLD3 co-crystallized with 5'Pi-ssDNA for 9 days

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	5'-3' exonuclease PLD3	polymer	467	51935.2	2	UniProt (O35405) Pfam (PF13918) In PDB	Mus musculus (house mouse)
2	B, D	5'-3' exonuclease PLD3	polymer	467	51856.2	2	UniProt (O35405) Pfam (PF13918) In PDB	Mus musculus (house mouse)
3	E, I	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	F, G	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
5	H	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	1	In PDB GlyTouCan (G55220VL)
6	A, B, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
7	B, D	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
8		water	water		18.0	187	Chemie (HOH)

Sequence modifications

A, C: 63 - 488 (UniProt: O35405)

PDB	External Database	Details
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
Gly 28	-	expression tag
Pro 29	-	expression tag
Leu 30	-	expression tag
Val 31	-	expression tag
Asp 32	-	expression tag
Val 33	-	expression tag
Ala 34	-	expression tag
Ser 35	-	expression tag
Asn 36	-	expression tag
Glu 37	-	expression tag
Gln 38	-	expression tag
Lys 39	-	expression tag
Leu 40	-	expression tag
Ile 41	-	expression tag
Ser 42	-	expression tag
Glu 43	-	expression tag
Glu 44	-	expression tag
Asp 45	-	expression tag
Leu 46	-	expression tag
Ala 47	-	expression tag
Ser 48	-	expression tag
Met 49	-	expression tag
Thr 50	-	expression tag
Gly 51	-	expression tag
Gly 52	-	expression tag
Gln 53	-	expression tag
Gln 54	-	expression tag
Met 55	-	expression tag
Gly 56	-	expression tag
Arg 57	-	expression tag
Asp 58	-	expression tag
Ile 59	-	expression tag
Glu 60	-	expression tag
Gly 61	-	expression tag
Arg 62	-	expression tag

B, D: 63 - 488 (UniProt: O35405)

PDB	External Database	Details
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
Gly 28	-	expression tag
Pro 29	-	expression tag
Leu 30	-	expression tag
Val 31	-	expression tag
Asp 32	-	expression tag
Val 33	-	expression tag
Ala 34	-	expression tag
Ser 35	-	expression tag
Asn 36	-	expression tag
Glu 37	-	expression tag
Gln 38	-	expression tag
Lys 39	-	expression tag
Leu 40	-	expression tag
Ile 41	-	expression tag
Ser 42	-	expression tag
Glu 43	-	expression tag
Glu 44	-	expression tag
Asp 45	-	expression tag
Leu 46	-	expression tag
Ala 47	-	expression tag
Ser 48	-	expression tag
Met 49	-	expression tag
Thr 50	-	expression tag
Gly 51	-	expression tag
Gly 52	-	expression tag
Gln 53	-	expression tag
Gln 54	-	expression tag
Met 55	-	expression tag
Gly 56	-	expression tag
Arg 57	-	expression tag
Asp 58	-	expression tag
Ile 59	-	expression tag
Glu 60	-	expression tag
Gly 61	-	expression tag
Arg 62	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	207582.7
Branched	Number of molecules	5
	Total formula weight	3257.0
Non-Polymers*	Number of molecules	5
	Total formula weight	853.6
All*	Total formula weight	211693.3

*Water molecules are not included.