8UT9

CryoEM structure of A/Shanghai/1/2013 H7 in complex with polyclonal Fab from mice immunized with H7 stem nanoparticles-28 days post immunization

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Hemagglutinin HA1 chain	polymer	317	34582.9	3	UniProt (V5IRV0) Pfam (PF00509) In PDB	Influenza A virus
2	B, D, F	Hemagglutinin HA2 chain	polymer	231	26598.2	3	UniProt (A0A881CR78) Pfam (PF00509) In PDB	Influenza A virus
3	G	H7D28 pFab HC Fv_polyA	polymer	126	10741.2	1		Mus musculus
4	H	H7D28 pFab LC Fv_polyA	polymer	108	9209.3	1		Mus musculus
5	I	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G79924RM)
6	J, K	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
7	A, B, C, D, E...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	9	Chemie (NAG)

Sequence modifications

A, C, E: 1 - 316 (UniProt: V5IRV0)

PDB	External Database	Details
Phe 88	Tyr 88	conflict
Ile 317	-	expression tag

B, D, F: -3 - 174 (UniProt: A0A881CR78)

PDB	External Database	Details
Thr 71	Asn 410	conflict
Gly 175	-	expression tag
Ser 176	-	expression tag
Gly 177	-	expression tag
Tyr 178	-	expression tag
Ile 179	-	expression tag
Pro 180	-	expression tag
Glu 181	-	expression tag
Ala 182	-	expression tag
Pro 183	-	expression tag
Arg 184	-	expression tag
Asp 185	-	expression tag
Gly 186	-	expression tag
Gln 187	-	expression tag
Ala 188	-	expression tag
Tyr 189	-	expression tag
Val 190	-	expression tag
Arg 191	-	expression tag
Lys 192	-	expression tag
Asp 193	-	expression tag
Gly 194	-	expression tag
Glu 195	-	expression tag
Trp 196	-	expression tag
Val 197	-	expression tag
Leu 198	-	expression tag
Leu 199	-	expression tag
Ser 200	-	expression tag
Thr 201	-	expression tag
Phe 202	-	expression tag
Leu 203	-	expression tag
Gly 204	-	expression tag
Ser 205	-	expression tag
Gly 206	-	expression tag
Leu 207	-	expression tag
Asn 208	-	expression tag
Asp 209	-	expression tag
Ile 210	-	expression tag
Phe 211	-	expression tag
Glu 212	-	expression tag
Ala 213	-	expression tag
Gln 214	-	expression tag
Lys 215	-	expression tag
Ile 216	-	expression tag
Glu 217	-	expression tag
Trp 218	-	expression tag
His 219	-	expression tag
Glu 220	-	expression tag
Gly 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	203494.0
Branched	Number of molecules	3
	Total formula weight	1273.2
Non-Polymers*	Number of molecules	9
	Total formula weight	1990.9
All*	Total formula weight	206758.0

*Water molecules are not included.